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Structure type: oligomer
Aglycon: remaining core-lipid A
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130693,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_983931,SB_192

• Article ID: 340
  Nnalue NA "a-GlcNAc-1→2-a-Glc, the Salmonella homologue of a conserved lipopolysaccharide motif in the Enterobacteriaceae, elicits broadly cross-reactive antibodies" - Infection and Immunity 66(9) (1998) 4389-4396
  

  To define cross-reactive epitopes in Salmonella lipopolysaccharide (LPS), antisera designated anti-S, anti-Ra, and anti-Re were generated against smooth (S), complete-core (Ra), and deep-core mutant (Re) strains, respectively, and characterized immunochemically. The reactivities of anti-Ra and anti-S with rough LPS (rLPS) chemotypes in enzyme-linked immunosorbent assays (ELISA) decreased progressively with increasing truncation of the complete-core oligosaccharide (e.g., Ra > Rb1 >.Re), while that of anti-Re increased (Ra < Rb1 <.Re). Anti-Ra was relatively more reactive with nonhomologous smooth LPS (sLPS) than anti-S, which in turn was more reactive than anti-Re. This order reflected the relative reactivities of these sera with outer-core rLPS but not those with inner-core rLPS, which suggests that the cross-reactivities of all three sera with sLPS were mediated by antibodies which bind outer-core determinants. Anti-Ra, but not anti-S or anti-Re, reacted with molecules substituted by O chains in immunoblots and revealed ladder-like patterns in sLPSs of various serospecificities. Anti-Ra, however, did not react with O-antigen-specific neoglycoconjugates in ELISA, thus demonstrating specificity for core epitopes. Ra and Rb1 but not other Salmonella core chemotypes inhibited the reactivity of anti-Ra with sLPS in ELISA, which showed that the terminal outer-core disaccharide, αGlcNAc 1→2 αGlc (GlcNAc → Glc), was the major epitope of cross-reactive antibodies in the serum. GlcNAc → Glc represents the conserved motif α-hexose 1→2 α-hexose in cores of the Enterobacteriaceae, other homologues of which should likewise be cross-reactive. These results demonstrate that S or Re strains do not elicit cross-reactive antibodies and indicate that immunization with Ra strains may represent a general strategy for eliciting cross-reactive antibodies against LPSs from enteric bacteria

  Lipopolysaccharide, antibodies, antibody, conserved, Salmonella, motif, Enterobacteriaceae, cross-reactive

  NCBI PubMed ID: 9712792
  Journal NLM ID: 0246127
  Publisher: American Society for Microbiology
  Correspondence: nnnalue@kumc.edu
  Institutions: Department of Medical Microbiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates
  Methods: SDS-PAGE, ELISA, immunoblotting, stereoplotting
  
   
  
  Salmonella
  CSDB ID 7763 (all data & tools)