Three new cycloartane-type triterpene glycosides, brachyosides A (1), B (3), and C (2), from the roots of Astragalus brachypterus and one new glycoside, cyclocephaloside II (4), from the roots of Astragalusmicrocephalus have been isolated together with five known saponins, astragalosides I, II, and IV, cyclocanthoside E, and cycloastragenol. The structures of the new compounds were established as 3-O-[beta-D-xylopyranosyl(1-->3)-beta-D-xylopyranosyl-6-O-beta-D-gluc opyranosyl-3beta,6alpha,16beta,24(S),25-pentahydrox ycycloartane (1), 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-24-O-beta-D-glucop yranosyl-3beta,6alpha,16beta,24(S),25-pentahydroxyc ycloartane (2), 20(R),24(S)-epoxy-6-O-beta-D-glucopyranosyl-3beta,6alpha,16beta , 25-tetrahydroxycycloartane (3), and 20(R), 24(S)-epoxy-3-O-(4'-O-acetyl)-beta-D-xylopyranosyl-6-O-beta-D-glucopy ranosyl-3beta,6alpha,16beta,25-tetrahydroxycycloart ane (4). For the structure elucidations, 1D- and 2D-NMR experiments and FABMS were used.

NMR, spectroscopy

• Compound ID: 27852
  

  b-D-Glcp-(1-6)-+ | b-D-Xylp2Ac3Ac-(1-3)-Subst Subst = astramembrangenin = SMILES CC1(C){3}[C@@H](O)CC[C@]2(C3)C43CC[C@]5(C)[C@@H]([C@](C)(O6)CC[C@H]6{25}C(C)(O)C){16}[C@@H](O)C[C@](C)5[C@]4([H])C{6}[C@H](O)[C@@]12[H]

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  Structure type: oligomer
  C41H68O14
  Trivial name: astragaloside I, astragaloside I (astrasieversianin IV)
  Compound class: saponin glycoside, glycoside, triterpenoid glycoside
  
   
  
  Astragalus microcephalus
  CSDB ID 61482 (all data & tools)
• Compound ID: 28878
  

  b-D-Glcp-(1-6)-+ | b-D-Xylp-(1-3)-Subst Subst = 3β,6α,16β,24(S),25-pentahydroxycycloartane = SMILES C[C@H](CC{24}[C@@H](O){25}C(C)(C)O)C4{16}[C@@H](O)C[C@@]5(C)C2C{6}[C@H](O)C1[C@@](C)(C){3}[C@@H](O)CC[C@@]13C[C@@]23CC[C@]45C

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  Structure type: oligomer
  C30H52O5
  Trivial name: cyclocanthoside E
  Compound class: saponin glycoside, triterpenoid glycoside
  
   
  
  Astragalus microcephalus
  CSDB ID 61485 (all data & tools)
• Compound ID: 28879
  

  b-D-Glcp-(1-6)-+ | b-D-Xylp-(1-3)-Subst Subst = astramembrangenin = SMILES CC1(C){3}[C@@H](O)CC[C@]2(C3)C43CC[C@]5(C)[C@@H]([C@](C)(O6)CC[C@H]6{25}C(C)(O)C){16}[C@@H](O)C[C@](C)5[C@]4([H])C{6}[C@H](O)[C@@]12[H]

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  Structure type: oligomer
  Trivial name: astragaloside IV, astragalus saponin AS I, cycloastragenol
  Compound class: saponin glycoside, glycoside, triterpenoid glycoside
  
   
  
  Astragalus microcephalus
  CSDB ID 61484 (all data & tools)
• Compound ID: 28882
  

  b-D-Glcp-(1-6)-+ | b-D-Xylp-(1-3)-b-D-Xylp-(1-3)-Subst Subst = 3β,6α,16β,24(S),25-pentahydroxycycloartane = SMILES C[C@H](CC{24}[C@@H](O){25}C(C)(C)O)C4{16}[C@@H](O)C[C@@]5(C)C2C{6}[C@H](O)C1[C@@](C)(C){3}[C@@H](O)CC[C@@]13C[C@@]23CC[C@]45C

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  Structure type: oligomer ; 917 [M-H]-
  C46H78O18
  Trivial name: brachyoside A
  Compound class: triterpenoid glycoside
  
   
  
  Astragalus microcephalus
  CSDB ID 61478 (all data & tools)
• Compound ID: 28883
  

  b-D-Xylp-(1-3)-+ | b-D-Glcp-(1-24)-Subst | b-D-Glcp-(1-6)-+ Subst = 3β,6α,16β,24(S),25-pentahydroxycycloartane = SMILES C[C@H](CC{24}[C@@H](O){25}C(C)(C)O)C4{16}[C@@H](O)C[C@@]5(C)C2C{6}[C@H](O)C1[C@@](C)(C){3}[C@@H](O)CC[C@@]13C[C@@]23CC[C@]45C

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  Structure type: oligomer ; 947 [M-H]-
  C47H80O19
  Trivial name: brachyoside C
  Compound class: triterpenoid glycoside
  
   
  
  Astragalus microcephalus
  CSDB ID 61479 (all data & tools)
• Compound ID: 28884
  

  b-D-Glcp-(1-6)-Subst Subst = 3β,6α,16β,24(S),25-pentahydroxycycloartane = SMILES C[C@H](CC{24}[C@@H](O){25}C(C)(C)O)C4{16}[C@@H](O)C[C@@]5(C)C2C{6}[C@H](O)C1[C@@](C)(C){3}[C@@H](O)CC[C@@]13C[C@@]23CC[C@]45C

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  Structure type: monomer ; 651
  C36H60O10
  Trivial name: brachyoside B
  Compound class: triterpenoid glycoside
  
   
  
  Astragalus microcephalus
  CSDB ID 61480 (all data & tools)
• Compound ID: 28885
  

  b-D-Glcp-(1-6)-+ | b-D-Xylp4Ac-(1-3)-Subst Subst = 3β,6α,16β,24(S),25-pentahydroxycycloartane = SMILES C[C@H](CC{24}[C@@H](O){25}C(C)(C)O)C4{16}[C@@H](O)C[C@@]5(C)C2C{6}[C@H](O)C1[C@@](C)(C){3}[C@@H](O)CC[C@@]13C[C@@]23CC[C@]45C

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  Structure type: oligomer ; 825 [M-H]-
  C43H70O15
  Trivial name: cyclocephaloside II
  Compound class: triterpenoid glycoside
  
   
  
  Astragalus microcephalus
  CSDB ID 61481 (all data & tools)
• Compound ID: 28886
  

  b-D-Glcp-(1-6)-+ | b-D-Xylp2Ac-(1-3)-Subst Subst = astramembrangenin = SMILES CC1(C){3}[C@@H](O)CC[C@]2(C3)C43CC[C@]5(C)[C@@H]([C@](C)(O6)CC[C@H]6{25}C(C)(O)C){16}[C@@H](O)C[C@](C)5[C@]4([H])C{6}[C@H](O)[C@@]12[H]

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  Structure type: oligomer
  Trivial name: astragaloside II
  Compound class: saponin glycoside, glycoside, triterpenoid glycoside
  
   
  
  Astragalus microcephalus
  CSDB ID 61483 (all data & tools)