Acetolysis of Candida parapsilosis cell-wall D-mannan, obtained by alkali extraction and purified as a copper complex, gave six oligosaccharides by Bio-Gel P-2 filtration. Their chemical structure was examined by 1H-n.m.r. spectroscopy, methylation analysis, and partial acid hydrolysis. The structures of the penta- and hexa-saccharide were α-D-manp-(1 leads to 3)-α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 2)-D-Man (M5) and α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 3)-α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 2)-D-Man (M6), respectively. Inhibition of the oligosaccharides with anti-C. parapsilosis serum and homologous D-mannan indicated that although M5 and M6 exhibited strong inhibitory-activity, M6 was the more effective inhibitor, and suggested that M6 may be the immunodominant or may be responsible for the specificity of C. parapsilosis mannan in the precipitin-reaction system, or both.
Acetolysis of Candida parapsilosis cell-wall D-mannan, obtained by alkali extraction and purified as a copper complex, gave six oligosaccharides by Bio-Gel P-2 filtration. Their chemical structure was examined by 1H-n.m.r. spectroscopy, methylation analysis, and partial acid hydrolysis. The structures of the penta- and hexa-saccharide were α-D-manp-(1 leads to 3)-α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 2)-D-Man (M5) and α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 3)-α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 2)-D-Man (M6), respectively. Inhibition of the oligosaccharides with anti-C. parapsilosis serum and homologous D-mannan indicated that although M5 and M6 exhibited strong inhibitory-activity, M6 was the more effective inhibitor, and suggested that M6 may be the immunodominant or may be responsible for the specificity of C. parapsilosis mannan in the precipitin-reaction system, or both.
Related record ID(s): 113860, 113862, 113863 NCBI Taxonomy refs (TaxIDs):5480 Reference(s) to other database(s): GTC:G96676KK, CCSD:9946, CBank-STR:14912 Show glycosyltransferases
There are 3 chemically distinct structures. Please, select:
Acetolysis of Candida parapsilosis cell-wall D-mannan, obtained by alkali extraction and purified as a copper complex, gave six oligosaccharides by Bio-Gel P-2 filtration. Their chemical structure was examined by 1H-n.m.r. spectroscopy, methylation analysis, and partial acid hydrolysis. The structures of the penta- and hexa-saccharide were α-D-manp-(1 leads to 3)-α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 2)-D-Man (M5) and α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 3)-α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 2)-D-Man (M6), respectively. Inhibition of the oligosaccharides with anti-C. parapsilosis serum and homologous D-mannan indicated that although M5 and M6 exhibited strong inhibitory-activity, M6 was the more effective inhibitor, and suggested that M6 may be the immunodominant or may be responsible for the specificity of C. parapsilosis mannan in the precipitin-reaction system, or both.
Related record ID(s): 104622, 113860, 113861, 113863, 115626, 116245, 130428, 130517, 143579 NCBI Taxonomy refs (TaxIDs):5480 Reference(s) to other database(s): GTC:G13237OG, CCSD:9943, CBank-STR:12456 Show glycosyltransferases
There are 3 chemically distinct structures. Please, select:
Acetolysis of Candida parapsilosis cell-wall D-mannan, obtained by alkali extraction and purified as a copper complex, gave six oligosaccharides by Bio-Gel P-2 filtration. Their chemical structure was examined by 1H-n.m.r. spectroscopy, methylation analysis, and partial acid hydrolysis. The structures of the penta- and hexa-saccharide were α-D-manp-(1 leads to 3)-α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 2)-D-Man (M5) and α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 3)-α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 2)-α-D-manp-(1 leads to 2)-D-Man (M6), respectively. Inhibition of the oligosaccharides with anti-C. parapsilosis serum and homologous D-mannan indicated that although M5 and M6 exhibited strong inhibitory-activity, M6 was the more effective inhibitor, and suggested that M6 may be the immunodominant or may be responsible for the specificity of C. parapsilosis mannan in the precipitin-reaction system, or both.
Related record ID(s): 113860, 113861, 113862 NCBI Taxonomy refs (TaxIDs):5480 Reference(s) to other database(s): GTC:G89131OO, CCSD:9944, CBank-STR:12455 Show glycosyltransferases
There are 3 chemically distinct structures. Please, select:
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