Glycopolymers with oligosaccharyl phosphate repeats of two types and ribitol and glycerol teichoic acids were isolated from cell wall of Bifidobacterium longum BIM B-476-D by stepwise extraction with 10% CCl3CO2H. The following structures of the glycopolymers were established by sugar analysis, selective cleavage with aq 2% HOAc, dephosphorylation with 48% HF, 2D NMR spectroscopy, and high-resolution ESI MS: [structure: see text]. The ribitol teichoic acid also contains minor D-alanine, whose position was not determined.
structure, teichoic acid, Bifidobacterium longum, glycopolymers
NCBI PubMed ID: 23571164Publication DOI: 10.1016/j.carres.2013.03.006Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: E.L. Zdorovenko
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, 220141 Minsk, Belarus, Institute of Microbiology, National Academy of Sciences of Belarus, 220141 Minsk, Belarus
Methods: 13C NMR, 1H NMR, NMR-2D, HF solvolysis, sugar analysis, ESI-MS, GLC, mild acid hydrolysis, NMR-1D
Found