Xu Z, Chen G, Xue L, Zhang H, Wang J, Xiang H, Li J, Zheng K Isolation, structural characterizations and bioactivities of exopolysaccharides produced by Bacillus licheniformis International Journal of Biological Macromolecules141 (2019)
298-306
The structure was elucidated in this paper NCBI PubMed ID:31465808 Publication DOI:10.1016/j.ijbiomac.2019.08.217 Journal NLM ID:7909578 Publisher: Butterworth-Heinemann Correspondence: K. Zheng <jnzhengkai163.com> Institutions: School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250300, China, CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China, Yunnan Academy of Tobacco Sciences, Kunming 650106, China, Yunnan Academy of Tobacco Sciences, Kunming 650106, China
Extraction polysaccharide from microorganism is a research hotspot. In this work, a new type of water-soluble exopolysaccharides (EPS) was isolated from Bacillus licheniformis. Firstly, response surface methodology (RSM), based on a three-level, three-factor, was used to determine optimum conditions for EPS extraction. And RSM analysis indicated optimum condition was at the temperature of 8 °C for 10.44 h with ethanol at a concentration of 79.22% (v/v), the maximum yield of EPS was 3.07 g/mL. Secondly, EPS were seperated using DEAE-Sepharose Fast Flow column chromatography and acquired two polysaccharide fractions, BL-P1 and BL-P2. BL-P1 had larger molecular weight than BL-P2 from structural analyses, because of higher content of mannose, ribose, glucuronic acid, galactose, arabinose and fructose in BL-P2. Moreover, the characterization of BL-P1 and BL-P2 was investigated with Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance (NMR) spectroscopy, the results indicated that EPS was mainly composed of →3)-α-d-Galp-(1→, →3,5)-α-l-Araf-(1→, →3)-β-d-Glcp-(1→, β-d-Glcp-(1→ and →4)-β-l-Fucp-(1→4)-β-d-Xylp-(1→4)-α-l-Rhap(1→3)-β-d-Manp-(4→ residues. In vitro antioxidant activity assay, EPS exhibited potent quenching capacities on hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals in a dose-dependent manner. Furthermore, BL-P2 had higher activity than BL-P1 in inhibiting α-amylase and α-glucosidase, which would have potential to be applied in nutraceutical and pharmaceutical industries.
Methods: 13C NMR, 1H NMR, FTIR, composition analysis, HPLC, extraction, statistical analysis, HPGPC, antioxidant activity assay, hypoglycemic assay Comments, role: aLFucp are erroneously depicted as bLFucp in the abstract, on pages 303 and 305. Published erroneous NMR chemical shifts of #3,4,4_aLFucp C4 (71.71) and #3_aLRhap C4 (73.48) were removed by CSDB staff
Related record ID(s): 2355, 2356 NCBI Taxonomy refs (TaxIDs):1402 Reference(s) to other database(s): GTC:G38903OT Show glycosyltransferases
Xu Z, Chen G, Xue L, Zhang H, Wang J, Xiang H, Li J, Zheng K Isolation, structural characterizations and bioactivities of exopolysaccharides produced by Bacillus licheniformis International Journal of Biological Macromolecules141 (2019)
298-306
The structure was elucidated in this paper NCBI PubMed ID:31465808 Publication DOI:10.1016/j.ijbiomac.2019.08.217 Journal NLM ID:7909578 Publisher: Butterworth-Heinemann Correspondence: K. Zheng <jnzhengkai163.com> Institutions: School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250300, China, CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China, Yunnan Academy of Tobacco Sciences, Kunming 650106, China, Yunnan Academy of Tobacco Sciences, Kunming 650106, China
Extraction polysaccharide from microorganism is a research hotspot. In this work, a new type of water-soluble exopolysaccharides (EPS) was isolated from Bacillus licheniformis. Firstly, response surface methodology (RSM), based on a three-level, three-factor, was used to determine optimum conditions for EPS extraction. And RSM analysis indicated optimum condition was at the temperature of 8 °C for 10.44 h with ethanol at a concentration of 79.22% (v/v), the maximum yield of EPS was 3.07 g/mL. Secondly, EPS were seperated using DEAE-Sepharose Fast Flow column chromatography and acquired two polysaccharide fractions, BL-P1 and BL-P2. BL-P1 had larger molecular weight than BL-P2 from structural analyses, because of higher content of mannose, ribose, glucuronic acid, galactose, arabinose and fructose in BL-P2. Moreover, the characterization of BL-P1 and BL-P2 was investigated with Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance (NMR) spectroscopy, the results indicated that EPS was mainly composed of →3)-α-d-Galp-(1→, →3,5)-α-l-Araf-(1→, →3)-β-d-Glcp-(1→, β-d-Glcp-(1→ and →4)-β-l-Fucp-(1→4)-β-d-Xylp-(1→4)-α-l-Rhap(1→3)-β-d-Manp-(4→ residues. In vitro antioxidant activity assay, EPS exhibited potent quenching capacities on hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals in a dose-dependent manner. Furthermore, BL-P2 had higher activity than BL-P1 in inhibiting α-amylase and α-glucosidase, which would have potential to be applied in nutraceutical and pharmaceutical industries.
Xu Z, Chen G, Xue L, Zhang H, Wang J, Xiang H, Li J, Zheng K Isolation, structural characterizations and bioactivities of exopolysaccharides produced by Bacillus licheniformis International Journal of Biological Macromolecules141 (2019)
298-306
The structure was elucidated in this paper NCBI PubMed ID:31465808 Publication DOI:10.1016/j.ijbiomac.2019.08.217 Journal NLM ID:7909578 Publisher: Butterworth-Heinemann Correspondence: K. Zheng <jnzhengkai163.com> Institutions: School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250300, China, CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China, Yunnan Academy of Tobacco Sciences, Kunming 650106, China, Yunnan Academy of Tobacco Sciences, Kunming 650106, China
Extraction polysaccharide from microorganism is a research hotspot. In this work, a new type of water-soluble exopolysaccharides (EPS) was isolated from Bacillus licheniformis. Firstly, response surface methodology (RSM), based on a three-level, three-factor, was used to determine optimum conditions for EPS extraction. And RSM analysis indicated optimum condition was at the temperature of 8 °C for 10.44 h with ethanol at a concentration of 79.22% (v/v), the maximum yield of EPS was 3.07 g/mL. Secondly, EPS were seperated using DEAE-Sepharose Fast Flow column chromatography and acquired two polysaccharide fractions, BL-P1 and BL-P2. BL-P1 had larger molecular weight than BL-P2 from structural analyses, because of higher content of mannose, ribose, glucuronic acid, galactose, arabinose and fructose in BL-P2. Moreover, the characterization of BL-P1 and BL-P2 was investigated with Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance (NMR) spectroscopy, the results indicated that EPS was mainly composed of →3)-α-d-Galp-(1→, →3,5)-α-l-Araf-(1→, →3)-β-d-Glcp-(1→, β-d-Glcp-(1→ and →4)-β-l-Fucp-(1→4)-β-d-Xylp-(1→4)-α-l-Rhap(1→3)-β-d-Manp-(4→ residues. In vitro antioxidant activity assay, EPS exhibited potent quenching capacities on hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals in a dose-dependent manner. Furthermore, BL-P2 had higher activity than BL-P1 in inhibiting α-amylase and α-glucosidase, which would have potential to be applied in nutraceutical and pharmaceutical industries.
report error
Found 3 records.
Displayed records from 1 to 3
163.com>