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1. (Article ID: 1155)
Rougeaux H, Kervarec N, Pichon R, Guezennec J
Structure of the exopolysaccharide of Vibrio diabolicus isolated from a deep-sea hydrothermal vent
Carbohydrate Research 322(1-2) (1999)
40-45
The structure of the exopolysaccharide produced under laboratory conditions by Vibrio diabolicus, a bacterium recovered from a deep-sea hydrothermal vent, has been investigated using sugar and methylation analysis and NMR spectroscopy. The polysaccharide consists of a linear tetrasaccharide repeating unit with the following structure [→3)-β-D-GlcpNac-(1→4)-β-D-GlcpA-(1→4)-β-D-GlcpA-(1→4)-α-D-GalpNAc-(1→]
structure, uronic acid, exopolysaccharide, Bacterial polysaccharide, Vibrio, glucuronic acid, hydrothermal, hydrothermal vent, amino sugars
NCBI PubMed ID: 10629947Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: jguezenn
ifremer.fr
Institutions: Laboratoire de RMN, Uni6ersite de Bretagne Occidentale, F-29200 Brest, France, Ifremer, Centre de Brest, DRV:VP:BMM, BP 70, F-29280 Plouzane, France
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, composition analysis
The publication contains the following compound(s):
- Compound ID: 3163
|
-3)-b-D-GlcpNAc-(1-4)-b-D-GlcpA-(1-4)-b-D-GlcpA-(1-4)-a-D-GalpNAc-(1- |
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Structure type: polymer chemical repeating unit
Trivial name: GAG-like HE800 EPS
Compound class: EPS, polysaccharide
Reference(s) to other database(s): GTC:G52037ZB, GlycomeDB:
26281
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2. (Article ID: 12014)
Bailey NJC, Cooper P, Hadfield ST, Lenz EM, Lindon JC, Nicholson JK, Stanley PD, Wilson ID, Wright B, Taylor SD
Application of directly coupled HPLC-NMR-MS/MS to the identification of metabolites of 5-trifluoromethylpyridone (2-hydroxy-5-trifluoromethylpyridine) in hydroponically grown plants
Journal of Agricultural and Food Chemistry 48(1) (2000)
42-46
Directly coupled HPLC-NMR-MS was used to characterize two major metabolites of 5-trifluoromethylpyridone (2-hydroxy-5-trifluoromethylpyridine), a model compound for herbicides, after it had been dosed into hydroponically grown maize plants. The combination of NMR and MS data allowed the identification of both of these metabolites, namely, the N-glucoside and O-malonylglucoside conjugates of the parent pyridone. This work demonstrates the efficiency and the potential application of HPLC-NMR-MS to the investigation of the metabolism of agrochemicals. The work also indicates that combination of the use of hydroponically grown plants and directly coupled HPLC-NMR-MS allows rapid identification of metabolites with little sample preparation.
NMR, metabolism, MS, HPLC, 2-pyridone, hydroponics, xenobiotic
NCBI PubMed ID: 10637049Publication DOI: 10.1021/jf990387tJournal NLM ID: 0374755Publisher: American Chemical Society
Correspondence: j.nicholson
ic.ac.uk
Institutions: Biological Chemistry, Biomedical Sciences Division, Imperial College of Science, Technology and Medicine, Sir Alexander Fleming Building, London, UK, Astra Zeneca Agrochemicals, Jealotts Hill Research Station, Bracknell, UK, Safety of Medicines, Zeneca Pharmaceuticals, Nether Alderley, UK
Methods: 1H NMR, ESI-MS, biological assays, HPLC, UV, extraction, centrifugation, 19F NMR, HPLC-NMR-MS
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