Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperPublication DOI: 10.1016/j.carres.2016.03.011Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: yknirel
gmail.com
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China
O-polysaccharides of E. coli O28ab, O37, and O100 were found to contain glycerol 1-phosphate and the following structures of their oligosaccharide repeats were established by sugar analysis, Smith degradation (for O28ab), 1D and 2D (1)H, (13)C, and (13)P NMR spectroscopy: [Formula: see text]. Functions of putative glycosyltransferases genes in the O-antigen gene clusters of the strains studied were tentatively assigned based on similarities to genes of other E. coli O-serogroups available from GenBank and taking into account the O-polysaccharide structures established.
Lipopolysaccharide, Escherichia coli, O-polysaccharide, bacterial polysaccharide structure, O-antigen gene cluster, O-Polysaccharide structure, Glycerol 1-phosphate
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.31, chart 1, E. coli O100
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130695,IEDB_135813,IEDB_136105,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_141794,IEDB_141807,IEDB_143253,IEDB_151528,IEDB_151531,IEDB_190606,IEDB_225177,IEDB_885823,SB_7
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, 31P NMR, acid hydrolysis, GLC, Smith degradation, GPC, mild acid degradation, function analysis of gene clusters
NCBI Taxonomy refs (TaxIDs): 2067420Reference(s) to other database(s): GTC:G36774WT
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,3,3,0 xDGro 67.7 72.0 63.4
3,3,3,3 P
3,3,3 aLRhap 102.3 78.7 76.2 72.6 70.3 17.9
3,3 aLRhap 103.2 71.1 79.4 72.6 70.3 17.9
3 aDGalp 100.3 69.0 78.1 70.0 72.0 61.5
2 Ac 175.8 23.7
bDGlcpN 103.8 55.3 80.5 71.8 76.4 61.5
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,3,3,0 xDGro 3.91-4.00 3.91 3.61-3.67
3,3,3,3 P
3,3,3 aLRhap 5.20 4.33 4.35 3.40 3.85 1.28
3,3 aLRhap 5.00 4.16 3.92 3.58 3.85 1.28
3 aDGalp 5.43 3.95 3.84 4.07 3.73 3.73-3.73
2 Ac - 2.10
bDGlcpN 4.76 3.88 3.77 3.73 3.46 3.75-3.86
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,3,3,0 xDGro 67.7/3.91-4.00 72.0/3.91 63.4/3.61-3.67
3,3,3,3 P
3,3,3 aLRhap 102.3/5.20 78.7/4.33 76.2/4.35 72.6/3.40 70.3/3.85 17.9/1.28
3,3 aLRhap 103.2/5.00 71.1/4.16 79.4/3.92 72.6/3.58 70.3/3.85 17.9/1.28
3 aDGalp 100.3/5.43 69.0/3.95 78.1/3.84 70.0/4.07 72.0/3.73 61.5/3.73-3.73
2 Ac 23.7/2.10
bDGlcpN 103.8/4.76 55.3/3.88 80.5/3.77 71.8/3.73 76.4/3.46 61.5/3.75-3.86
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,3,3,0 | xDGro | 3.91
4.00 | 3.91 | 3.61
3.67 | |
| 3,3,3,3 | P | |
| 3,3,3 | aLRhap | 5.20 | 4.33 | 4.35 | 3.40 | 3.85 | 1.28 |
| 3,3 | aLRhap | 5.00 | 4.16 | 3.92 | 3.58 | 3.85 | 1.28 |
| 3 | aDGalp | 5.43 | 3.95 | 3.84 | 4.07 | 3.73 | 3.73
3.73 |
| 2 | Ac |
| 2.10 | |
| | bDGlcpN | 4.76 | 3.88 | 3.77 | 3.73 | 3.46 | 3.75
3.86 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,3,3,0 | xDGro | 67.7 | 72.0 | 63.4 | |
| 3,3,3,3 | P | |
| 3,3,3 | aLRhap | 102.3 | 78.7 | 76.2 | 72.6 | 70.3 | 17.9 |
| 3,3 | aLRhap | 103.2 | 71.1 | 79.4 | 72.6 | 70.3 | 17.9 |
| 3 | aDGalp | 100.3 | 69.0 | 78.1 | 70.0 | 72.0 | 61.5 |
| 2 | Ac | 175.8 | 23.7 | |
| | bDGlcpN | 103.8 | 55.3 | 80.5 | 71.8 | 76.4 | 61.5 |
|
There is only one chemically distinct structure:
Found 
report error