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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)

The structure was elucidated in this paper
NCBI PubMed ID: 1703456
Publication DOI: 10.1016/0008-6215(90)80019-y
Journal NLM ID: 0043535
Publisher: Elsevier
Institutions: Department of Immunochemistry, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.

On mild acid-catalysed degradation of the lipopolysaccharide from Hafnia alvei O39 followed by gel filtration of Sephadex G-50, the O-specific polysaccharide and three oligosaccharides were obtained, which represent the core substituted with 0-2 O-antigen repeating-units. On the basis of sugar and methylation analyses, 13C-n.m.r. data, solvolysis of the polysaccharide with anhydrous hydrogen fluoride, and computer-assisted 13C-n.m.r. analysis of the Smith-degraded polysaccharide, it was concluded that the biological repeating unit of the O39 antigen was Formula; see text

Structure type: polymer biological repeating unit
Location inside paper: abstract, table II, PS-I
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_135813,IEDB_136044,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_141807,IEDB_142487,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_190606,IEDB_221845,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_21,SB_6,SB_7,SB_88

Methods: 13C NMR, methylation, GLC-MS, gel filtration, HF solvolysis, sugar analysis, acid hydrolysis, GLC, Smith degradation, HPLC

Related record ID(s): 2632, 2633, 4937, 10389, 11682, 20649, 23242, 26890, 27435, 28337, 30360, 108742
NCBI Taxonomy refs (TaxIDs): 569
Reference(s) to other database(s): GTC:G92807QP, GlycomeDB:3591
Show glycosyltransferases

NMR conditions: in D2O at 333(C) K [as TSV]

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
3,4,4	bDGalp	105.1	72.7	74.2	70.0	76.1	62.1
3,4	bDGalp	104.0	71.7	83.2	76.6	75.6	62.3
3,2,2	Ac
3,2	bDGlcpN	102.9	57.0	75.3	71.2	77.1	62.1
3	bDGlcp	103.7	80.1	77.1	79.2	75.6	61.0
2	Ac
	bDGalpN	104.3	52.5	81.6	69.0	76.0	62.3


1H NMR data:
missing...

¹³C NMR data:

Linkage	Residue	C1	C2	C3	C4	C5	C6
3,4,4	bDGalp	105.1	72.7	74.2	70.0	76.1	62.1
3,4	bDGalp	104.0	71.7	83.2	76.6	75.6	62.3
3,2,2	Ac
3,2	bDGlcpN	102.9	57.0	75.3	71.2	77.1	62.1
3	bDGlcp	103.7	80.1	77.1	79.2	75.6	61.0
2	Ac
	bDGalpN	104.3	52.5	81.6	69.0	76.0	62.3

There is only one chemically distinct structure:

*O[C@@H]1[C@@H](O)[C@H](O[C@H]2[C@H](O)[C@@H](O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3NC(C)=O)[C@H](O[C@H]3[C@@H](O)[C@@H](CO)O[C@@H](*)[C@@H]3NC(C)=O)O[C@@H]2CO)O[C@H](CO)[C@@H]1O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize