Six steroidal saponins were isolated from Anemarrhena asphodeloides Bunge (Liliaceae), a traditional chinese medicine, and named anemarrhenasaponin I (An-I), anemarrhenasaponin Ia (An-Ia), timosaponin B-I (TB-I), timosaponin B-II (TB-II), timosaponin B-III (TB-III), and timosaponin A-III (TA-III). The effects of these six compounds on platelet aggregation and hemolysis in human blood were studied. All these compounds provoked remarkable inhibiting effect on platelet aggregation, and activated partial thromboplastin times (APTT) are sensitive to the presence of these six compounds. Using an in vitro system, APTT was delayed with the increment of the concentrations of these six compounds. In these six compounds, only timosaponin A-III appeared a strong effect on hemolysis, and anemarrhenasaponin Ia had a slight effect on hemolysis, other had no effect on hemolysis. These results suggested that these steroidal saponins isolated from Anemarrhena asphodeloides Bunge (Liliaceae) might be used as a novel antithrombotic therapeutic agents in post-myocardial infarction.

steroidal saponins, hemolysis, Anemarrhenae rhizoma, platelet aggregation

• Compound ID: 26584
  

  b-D-Glcp-(1-2)-b-D-Galp-(1-3)-Subst Subst = sarsasapogenin = SMILES [H][C@]1(O[C@@]2(OC[C@@H](C)CC2)[C@H]3C)C[C@@]4([H])[C@]5([H])CC[C@]6([H])C{3}[C@@H](O)CC[C@]6(C)[C@@]5([H])CC[C@]4(C)[C@]13[H]

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  Structure type: oligomer
  C39H64O13
  Trivial name: timosaponin A-III
  Compound class: saponin glycoside
  Reference(s) to other database(s): CCSD:40014, CBank-STR:5811
  
   
  
  Anemarrhena asphodeloides
  CSDB ID 62953 (all data & tools)
• Compound ID: 27018
  

  b-D-Glcp-(1-26)-+ | b-D-Glcp-(1-2)-b-D-Galp-(1-3)-Subst Subst = 25S-5β-furostan-3β,22,26-triol = SMILES C[C@H]1[C@H]2[C@@H](O{22}C1(O)CC[C@@H]({26}[CH2]O)C)C[C@@H]3[C@]2(C)CC[C@H]4[C@H]3CC[C@H]5[C@]4(C)CC{3}[C@H](O)C5

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  Structure type: oligomer
  C45H76O19
  Trivial name: timosaponin B-II
  Compound class: saponin glycoside, glycoside
  Reference(s) to other database(s): CCSD:46115, CBank-STR:9412
  
   
  
  Anemarrhena asphodeloides
  CSDB ID 62951 (all data & tools)
• Compound ID: 30444
  

  b-D-Glcp-(1-2)-b-D-Galp-(1-3)-Subst22Me Subst = anemarrhenasaponin I aglycon = SMILES C[C@]12[C@]([H])([C@]3([C@]([H])(CC2)[C@]4(C)[C@]([H])(CC3)C{3}[C@H](CC4)O)[H]){15}[C@H]([C@]5([C@@]1([C@H](C){22}C(O)(O5)CCC(C)C)[H])[H])O

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  Structure type: oligomer
  C39H66O14
  Trivial name: anemarrhenasaponin I
  Compound class: saponin glycoside
  
   
  
  Anemarrhena asphodeloides
  CSDB ID 62948 (all data & tools)
• Compound ID: 30445
  

  b-D-Glcp-(1-2)-b-D-Galp-(1-3)-Subst Subst = anemarrhenasaponin I aglycon = SMILES C[C@]12[C@]([H])([C@]3([C@]([H])(CC2)[C@]4(C)[C@]([H])(CC3)C{3}[C@H](CC4)O)[H]){15}[C@H]([C@]5([C@@]1([C@H](C){22}C(O)(O5)CCC(C)C)[H])[H])O

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  Structure type: oligomer
  C40H68O14
  Trivial name: anemarrhenasaponin Ia
  Compound class: saponin glycoside
  
   
  
  Anemarrhena asphodeloides
  CSDB ID 62949 (all data & tools)
• Compound ID: 30446
  

  b-D-Glcp-(1-26)-+ | b-D-Glcp-(1-2)-b-D-Galp-(1-3)-Subst22Me Subst = 25S-5β-furostan-3β,22,26-triol = SMILES C[C@H]1[C@H]2[C@@H](O{22}C1(O)CC[C@@H]({26}[CH2]O)C)C[C@@H]3[C@]2(C)CC[C@H]4[C@H]3CC[C@H]5[C@]4(C)CC{3}[C@H](O)C5

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  Structure type: oligomer
  C46H78O19
  Trivial name: timosaponin B-I
  Compound class: saponin glycoside
  
   
  
  Anemarrhena asphodeloides
  CSDB ID 62950 (all data & tools)
• Compound ID: 30447
  

  b-D-Glcp-(1-26)-+ | b-D-Glcp-(1-2)-b-D-Galp-(1-3)-Subst Subst = (3β,5β,25S)-furost-20(22)-en-3,26-diol = SMILES [H][C@]1(OC(CC[C@H](C){26}CO)=C2C)C[C@@]3([H])[C@]4([H])CC[C@]5([H])C{3}[C@@H](O)CC[C@]5(C)[C@@]4([H])CC[C@]3(C)[C@]12[H]

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  Structure type: oligomer
  C45H74O18
  Trivial name: timosaponin B-III
  Compound class: saponin glycoside
  
   
  
  Anemarrhena asphodeloides
  CSDB ID 62952 (all data & tools)