Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: nosocomial infections [ICD11:
XB25 
];
infection due to Acinetobacter baumannii [ICD11:
XN8LS ]
The structure was elucidated in this paperPublication DOI: 10.1016/j.carres.2016.10.007Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: yknirel
gmail.com
Institutions: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia, School of Molecular Bioscience, The University of Sydney, Sydney, Australia, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia, M.M. Shemyakin & Y.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russi, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia, School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia
Capsular polysaccharides were recovered from four Acinetobacter baumannii isolates, and the following related structures of oligosaccharide repeating units were established by sugar analyses along with 1D and 2D 1H and 13C NMR spectroscopy: NIPH 60 and LUH5544 (K43) NIPH 601 (K47) The K locus for capsule biosynthesis in the genome sequences available for NIPH 60 and LUH5544, designated KL43, was found to be related to gene clusters KL47 in NIPH 601 and KL88 in LUH5548. The three clusters share most gene content differing in only a small portion that includes an additional glycosyltransferase genes in KL47 and KL88, as well as genes encoding distinct Wzy polymerases that were found to form the same α-d-GlcpNAc-(1→6)-α-d-GlcpNAc linkage in K43 and K47.
capsular polysaccharide, Acinetobacter baumannii, glycosyltransferase, Wzy polymerase, K locus
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.178, fig. 4A, K88
Compound class: CPS
Contained glycoepitopes: IEDB_130648,IEDB_135813,IEDB_137340,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_141807,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_885822,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, chemical analysis, acid hydrolysis, GLC, NMR-1D, bioinformatic analysis
Biosynthesis and genetic data: genetic data
Related record ID(s): 11361, 11791, 11793, 11794, 11795
NCBI Taxonomy refs (TaxIDs): 470Reference(s) to other database(s): GTC:G78379FN
Show glycosyltransferases
NMR conditions: in D2O at 293 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,4,6,6 bDGlcp 102.9 80.8 77.3 71.0 76.9 61.9
3,4,6,2 Ac 175.5-175.8 23.1-23.7
3,4,6 aDGlcpN 98.4 54.9 72.1 70.7 72.1 69.6
3,4,2 Ac 175.5-175.8 23.1-23.7
3,4 aDGlcpN 99.6 55.3 71.9 71.1 72.3 66.5
3,2 Ac 175.5-175.8 23.1-23.7
3 aDGalpN 98.6 51.1 68.2 78.0 72.8 60.9
2 Ac 175.5-175.8 23.1-23.7
bDGlcpN 102.3 55.9 79.9 72.3 77.1 62.1
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,6,6 bDGlcp 4.53 3.56 3.53 3.39 3.40 3.71-3.88
3,4,6,2 Ac - 2.03-2.08
3,4,6 aDGlcpN 4.90 3.95 3.79 3.79 3.79 3.94-4.12
3,4,2 Ac - 2.03-2.08
3,4 aDGlcpN 4.94 3.94 3.83 3.63 4.28 3.58-4.00
3,2 Ac - 2.03-2.08
3 aDGalpN 5.42 4.25 3.91 4.02 4.02 3.67-3.72
2 Ac - 2.03-2.08
bDGlcpN 4.89 3.84 3.74 3.67 3.43 3.77-3.92
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,4,6,6 bDGlcp 102.9/4.53 80.8/3.56 77.3/3.53 71.0/3.39 76.9/3.40 61.9/3.71-3.88
3,4,6,2 Ac 23.1-23.7/2.03-2.08
3,4,6 aDGlcpN 98.4/4.90 54.9/3.95 72.1/3.79 70.7/3.79 72.1/3.79 69.6/3.94-4.12
3,4,2 Ac 23.1-23.7/2.03-2.08
3,4 aDGlcpN 99.6/4.94 55.3/3.94 71.9/3.83 71.1/3.63 72.3/4.28 66.5/3.58-4.00
3,2 Ac 23.1-23.7/2.03-2.08
3 aDGalpN 98.6/5.42 51.1/4.25 68.2/3.91 78.0/4.02 72.8/4.02 60.9/3.67-3.72
2 Ac 23.1-23.7/2.03-2.08
bDGlcpN 102.3/4.89 55.9/3.84 79.9/3.74 72.3/3.67 77.1/3.43 62.1/3.77-3.92
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4,6,6 | bDGlcp | 4.53 | 3.56 | 3.53 | 3.39 | 3.40 | 3.71
3.88 |
| 3,4,6,2 | Ac |
| 2.03
2.08 | |
| 3,4,6 | aDGlcpN | 4.90 | 3.95 | 3.79 | 3.79 | 3.79 | 3.94
4.12 |
| 3,4,2 | Ac |
| 2.03
2.08 | |
| 3,4 | aDGlcpN | 4.94 | 3.94 | 3.83 | 3.63 | 4.28 | 3.58
4.00 |
| 3,2 | Ac |
| 2.03
2.08 | |
| 3 | aDGalpN | 5.42 | 4.25 | 3.91 | 4.02 | 4.02 | 3.67
3.72 |
| 2 | Ac |
| 2.03
2.08 | |
| | bDGlcpN | 4.89 | 3.84 | 3.74 | 3.67 | 3.43 | 3.77
3.92 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,4,6,6 | bDGlcp | 102.9 | 80.8 | 77.3 | 71.0 | 76.9 | 61.9 |
| 3,4,6,2 | Ac | 175.5
175.8 | 23.1
23.7 | |
| 3,4,6 | aDGlcpN | 98.4 | 54.9 | 72.1 | 70.7 | 72.1 | 69.6 |
| 3,4,2 | Ac | 175.5
175.8 | 23.1
23.7 | |
| 3,4 | aDGlcpN | 99.6 | 55.3 | 71.9 | 71.1 | 72.3 | 66.5 |
| 3,2 | Ac | 175.5
175.8 | 23.1
23.7 | |
| 3 | aDGalpN | 98.6 | 51.1 | 68.2 | 78.0 | 72.8 | 60.9 |
| 2 | Ac | 175.5
175.8 | 23.1
23.7 | |
| | bDGlcpN | 102.3 | 55.9 | 79.9 | 72.3 | 77.1 | 62.1 |
|
There is only one chemically distinct structure:
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