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1. (Article ID: 11849)
 
Ye W, Pan G, Zhang Q, Che CT, Wu H, Xhao S
Five new triterpene saponins from Pulsatilla patens var. multifida
Journal of Natural Products 62(2) (1999) 233-237
 

Five new oleanane-type glycosides (1−5), along with two known triterpene saponins, were isolated from the roots of Pulsatilla patens var. multifida (Ranunculaceae). The structures of the new triterpene saponins were elucidated as 3-O-β-D-glucopyranosyl(1→2)-β-D-galactopyranosyl hederagenin 28-O-β-D-glucopyranosyl ester (1), hederagenin 3-O-[β-D-glucopyranosyl(1→2)][β-D-glucopyranosyl(1→6)]-β-D-galactopyranoside (2), 3-O-β-D-glucopyranosyl bayogenin 28-O-α-L-rhamnopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranosyl ester (3), 3-O-β-D-glucopyranosyl(1→2)-β-D-galactopyranosyl oleanolic acid 28-O-α-L-rhamnopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranosyl ester (4), and 3-O-[β-D-glucopyranosyl(1→2)][β-D-glucopyranosyl(1→6)]-β-D-galactopyranosyl hederagenin 28-O-α-L-rhamnopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranosyl ester (5). Structure elucidation was accomplished by 1D and 2D NMR (HMQC, HMBC, and ROESY) methods, FABMS, and hydrolysis.

oleanane-type glycoside, triterpene saponin, Pulsatilla patens var. multifida

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