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Structure type: suggested polymer biological repeating unit
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115136,IEDB_135813,IEDB_137340,IEDB_140630,IEDB_141807,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_423153,IEDB_983931,SB_192

• Article ID: 4740
  Zdorovenko EL, Varbanets LD, Liu B, Valueva OA, Wang Q, Shashkov AS, Garkavaya EG, Brovarskaya OS, Wang L, Knirel YA "Structure and gene cluster of the O antigen of Escherichia coli L-19, a candidate for a new O-serogroup" - Microbiology 160(9) (2014) 2102-2107
  

  Escherichia coli L-19 isolated from a healthy individual did not agglutinate with any of 21 polyvalent antisera that cover 174 E. coli O-serogroups. The strain was studied in respect to the O-antigen (O-specific polysaccharide, OPS) structure and genetics. The LPS was isolated by phenol-water extraction of bacterial cells and cleaved by mild acid hydrolysis to yield the OPS. The OPS was studied by sugar and methylation analyses, along with 1D and 2D (1)H and (13)C NMR spectroscopy. The established structure of the linear tetrasaccharide repeating unit was found to be unique among known bacterial polysaccharide structures. A peculiar component of the L-19 OPS was an amide of glucuronic acid with 2-amino-1,3-propanediol (2-amino-2-deoxyglycerol) (GroN). The O-antigen gene cluster of L-19 between the conserved genes galF and gnd was sequenced, and gene functions were tentatively assigned by a comparison with sequences in the available databases and found to be in agreement with the OPS structure. Except for putative genes for synthesis and transfer of GroN, the sequences in the L-19 O-antigen gene cluster were little related to those of reference strains of the 174 known E. coli O-serogroups. The data obtained suggest that L-19 can be considered as a candidate for a new E. coli O-serogroup.

  NMR, structure, Escherichia coli, O-specific polysaccharide, O-antigen gene clustere

  NCBI PubMed ID: 25061042
  Publication DOI: 10.1099/mic.0.080804-0
  Journal NLM ID: 0376646
  WWW link: doi:10.1099/mic.0.080804-0
  Publisher: Washington, DC: Kluwer Academic/Plenum Publishers
  Correspondence: Evelina L. Zdorovenko
  Institutions: TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, 300457 Tianjin, PR China, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii Prospekt 47, Moscow, Russia, D. K. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Akademik Zabolotny Street 154, 03680 Kyiv, GSP, Ukraine
  Methods: 13C NMR, 1H NMR, methylation, NMR-2D, DNA sequencing, sugar analysis, mild acid hydrolysis, NMR-1D, function analysis of gene clusters, serotyping analysis
  
   
  
  Escherichia coli L-19
  CSDB ID 30565 (all data & tools)
• Article ID: 4753
  Chen M, Shpirt AM, Guo X, Shashkov AS, Wang L, Knirel YA, Liu B "Identification serologically, chemically and genetically of two Escherichia coli strains, as candidates for new O serogroups" - Microbiology 161(9) (2015) 1790-1796
  

  Escherichia coli strains are normally identified by the combination of their O and H (and sometimes K) antigens, and serotyping based on the antigens is believed to be crucial for clinical detection and epidemiological investigation. Two E. coli strains, G5413 and G5287, were isolated from faecal samples of female patients with diarrhoea and were not agglutinated with any antisera that cover the well-known O serogroups of E. coli. We elucidated the O-polysaccharide (OPS) structures and analysed the O-antigen gene clusters of these bacteria. The OPS structure of G5413 established by monosaccharide analysis and NMR spectroscopy was found to be unique amongst known bacterial polysaccharide structures. The O-antigen gene cluster of this strain was sequenced and did not match sequence data with any of the 184 O serogroups that have been recognized internationally. Gene functions were tentatively assigned and were appropriate to the OPS structure. Based on these data, we suggest G5413 as a candidate for a new E. coli O serogroup. Both the OPS structure and O-antigen gene cluster of G5287 were identical to those of E. coli L-19, a candidate for another new O serogroup characterized by us recently. Recognition of these two provisional O serogroups increases the number of known O-antigen forms of E. coli to 186.

  O-antigen, Escherichia coli, serotyping

  NCBI PubMed ID: 26297000
  Publication DOI: 10.1099/mic.0.000136
  Journal NLM ID: 0376646
  Publisher: Washington, DC: Kluwer Academic/Plenum Publishers
  Correspondence: Bin Liu
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii Prospekt 47, Moscow, Russia, Shanghai Municipal Center for Disease Control and Prevention, No. 1380, Zhong Shan Xi Road, Shanghai 200336, PR China, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin, China
  Methods: 13C NMR, 1H NMR, NMR-2D, DNA sequencing, sugar analysis, mild acid hydrolysis, GPC, serotyping, analysis of genes
  
   
  
  Escherichia coli G5287, Escherichia coli L-19
  CSDB ID 30919 (all data & tools)