Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: septicemia [ICD11:
MA15.Y 
];
infection due to Acinetobacter baumannii [ICD11:
XN8LS ]
The structure was elucidated in this paperNCBI PubMed ID: 28371606Publication DOI: 10.1134/S0006297917040101Journal NLM ID: 0376536Publisher: Nauka/Interperiodica
Correspondence: yknirel
gmail.com
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Moscow Region, Russia, Higher Chemical College of the Russian Academy of Sciences, Dmitry Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russia, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 119997 Moscow, Russia, Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Moscow Region, Russia, Department of Clinical Microbiology, Christian Medical College, Vellore, 632004 Tamil Nadu, India, Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
Capsular polysaccharide (CPS) assigned to the K93 type was isolated from the bacterium Acinetobacter baumannii B11911 and studied by sugar analysis along with one- and two-dimensional 1H and 13C NMR spectroscopy. The CPS was found to contain a derivative of pseudaminic acid, and the structure of the branched tetrasaccharide repeating unit was established. Genes in the KL93 capsule biosynthesis locus were annotated and found to be consistent with the CPS structure established. The K93 CPS has the α-d-Galp-(1→6)-β-d-Galp-(1→3)-d-GalpNAc trisaccharide fragment in common with the K14 CPS of Acinetobacter nosocomialis LUH 5541 and A. baumannii D46. It also shares the β-d-Galp-(1→3)-d-GalpNAc disaccharide fragment and the corresponding predicted Gal transferase Gtr5, as well as the initiating GalNAc-1-P transferase ItrA2, with a number of A. baumannii strains.
Acinetobacter baumannii, pseudaminic acid, capsular polysaccharide structure, glycosyltransferase, capsule biosynthesis locus
Structure type: polymer chemical repeating unit
-
Location inside paper: p.488, fig.4
Trivial name: K93 CPS
Compound class: CPS
Contained glycoepitopes: IEDB_130648,IEDB_134624,IEDB_134627,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_147450,IEDB_151528,IEDB_190606,IEDB_742248,IEDB_838988,SB_163,SB_165,SB_166,SB_187,SB_195,SB_21,SB_23,SB_24,SB_7,SB_8,SB_88
Methods: 13C NMR, 1H NMR, NMR-2D, acid hydrolysis, GLC, composition analysis, GPC, dialysis, bioinformatic analysis (BLASTp)
NCBI Taxonomy refs (TaxIDs): 470
Show glycosyltransferases
NMR conditions: in D2O at 338 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6 C7 C8 C9
3,6,6,7 lR3HOBut 174.6 46.4 66.3 23.6
3,6,6,5 Ac 175.7-176.2 23.6-23.8
3,6,6 bXPsep 174.1 102.6 37.1 68.1 49.9 74.7 54.9 70.9 19.2
3,6 aDGalp 100.1 69.7 70.7 70.7 70.8 65.1
3 bDGalp 106.0 71.4 82.7 69.9 73.7 67.9
2 Ac 175.7-176.2 23.6-23.8
bDGalpN 103.9 53.0 81.1 69.7 76.0 62.5
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6 H7 H8 H9
3,6,6,7 lR3HOBut - 2.35 4.16 1.22
3,6,6,5 Ac - 2.02-2.03
3,6,6 bXPsep - - 1.57-2.50 3.87 4.18 3.96 4.00 4.07 1.22
3,6 aDGalp 4.97 3.83 3.80 3.95 4.05 3.56-3.95
3 bDGalp 4.48 3.63 3.72 4.17 3.87 3.69-3.69
2 Ac - 2.02-2.03
bDGalpN 4.74 4.04 3.88 4.11 3.66 3.75-3.75
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6 C7/H7 C8/H8 C9/H9
3,6,6,7 lR3HOBut 46.4/2.35 66.3/4.16 23.6/1.22
3,6,6,5 Ac 23.6-23.8/2.02-2.03
3,6,6 bXPsep 37.1/1.57-2.50 68.1/3.87 49.9/4.18 74.7/3.96 54.9/4.00 70.9/4.07 19.2/1.22
3,6 aDGalp 100.1/4.97 69.7/3.83 70.7/3.80 70.7/3.95 70.8/4.05 65.1/3.56-3.95
3 bDGalp 106.0/4.48 71.4/3.63 82.7/3.72 69.9/4.17 73.7/3.87 67.9/3.69-3.69
2 Ac 23.6-23.8/2.02-2.03
bDGalpN 103.9/4.74 53.0/4.04 81.1/3.88 69.7/4.11 76.0/3.66 62.5/3.75-3.75
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 | H7 | H8 | H9 |
|---|
| 3,6,6,7 | lR3HOBut |
| 2.35 | 4.16 | 1.22 | |
| 3,6,6,5 | Ac |
| 2.02
2.03 | |
| 3,6,6 | bXPsep |
|
| 1.57
2.50 | 3.87 | 4.18 | 3.96 | 4.00 | 4.07 | 1.22 |
| 3,6 | aDGalp | 4.97 | 3.83 | 3.80 | 3.95 | 4.05 | 3.56
3.95 | |
| 3 | bDGalp | 4.48 | 3.63 | 3.72 | 4.17 | 3.87 | 3.69
3.69 | |
| 2 | Ac |
| 2.02
2.03 | |
| | bDGalpN | 4.74 | 4.04 | 3.88 | 4.11 | 3.66 | 3.75
3.75 | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 | C7 | C8 | C9 |
|---|
| 3,6,6,7 | lR3HOBut | 174.6 | 46.4 | 66.3 | 23.6 | |
| 3,6,6,5 | Ac | 175.7
176.2 | 23.6
23.8 | |
| 3,6,6 | bXPsep | 174.1 | 102.6 | 37.1 | 68.1 | 49.9 | 74.7 | 54.9 | 70.9 | 19.2 |
| 3,6 | aDGalp | 100.1 | 69.7 | 70.7 | 70.7 | 70.8 | 65.1 | |
| 3 | bDGalp | 106.0 | 71.4 | 82.7 | 69.9 | 73.7 | 67.9 | |
| 2 | Ac | 175.7
176.2 | 23.6
23.8 | |
| | bDGalpN | 103.9 | 53.0 | 81.1 | 69.7 | 76.0 | 62.5 | |
|
There is only one chemically distinct structure:
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