Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: nosocomial infections [ICD11:
XB25 
];
pneumonia [ICD11:
CA40 ];
bacteremia [ICD11:
MA15.0 ];
urinary tract infections (UTI) [ICD11:
GC08 ];
infection due to Acinetobacter baumannii [ICD11:
XN8LS ]
The structure was elucidated in this paperNCBI PubMed ID: 28190743Publication DOI: 10.1016/j.vaccine.2017.01.060Journal NLM ID: 8406899Publisher: Elsevier
Correspondence: shwu
gate.sinica.edu.tw
Institutions: Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan, National Health Research Institutes, Miaoli County 35053, Taiwan, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan, Department of Emergency Medicine, Taipei Veterans General Hospital, Taipei 112, Taiwan, Human Health Therapeutics, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A0R, Canada, Institute of Microbiology, Tzu Chi University, Hualien 907, Taiwan
Concerns of Acinetobacter baumannii infection have increased due to the emergence of multi-drug resistance. In the present study, we determined the capsular polysaccharide (CPS) structure of A. baumannii SK44, a clinical isolate from Taiwan, to consist of pentasaccharide repeats. We found that CPS-induced antibody provided 55% protection against challenge in an animal model. The CPS-specific antibody reacted with the surface components of about 62% clinical isolates (342/554 strains) from cross-sectional and longitudinal studies by dot-immunoassay. Pulsed-field gel electrophoresis of positive strains showed the antibody covered different clonalites of A. baumannii clinical isolates. Meanwhile, using the CPS antibody as a probe, we found a number of outer membrane proteins bound to the antibody, including OmpA/motB, TonB-dependent receptor, and Omp38, indicating their association with CPS. These results might lead to the use of the capsular polysaccharide as a vaccine to prevent A. baumannii infection.
capsular polysaccharide, Acinetobacter baumannii, Clinic population distribution, Passive immunity
Structure type: polymer chemical repeating unit
Location inside paper: p.1442, table S1
Trivial name: O-glycan
Compound class: CPS
Contained glycoepitopes: IEDB_130648,IEDB_135813,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_140529,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_167069,IEDB_190606,IEDB_983931,SB_192,SB_21,SB_7
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, sugar analysis, acid hydrolysis, MS/MS, serological methods, enzymatic digestion, statistical analysis, SEC, immunization, conjugation, pulsed-field gel electrophoresis (PFGE)
Comments, role: A. baumannii SK44 clinic strain;
Related record ID(s): 12266
NCBI Taxonomy refs (TaxIDs): 470,
400667Reference(s) to other database(s): GTC:G64997NS
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,6,4,2 Ac
3,6,4,3 Ac
3,6,4,4 Ac
3,6,4 bDGlcpN3NA 102.5 54.5 55.8 72.0 75.2 ?
3,6,6,2 Ac
3,6,6 bDGlcpN 103.0 56.7 75.2 71.0 76.9 61.9
3,6 aDGalp 99.2 68.5 81.7 78.1 70.6 72.3
3 bDGlcp 106.1 74.1 76.8 70.0 75.4 65.8
2 Ac
bDGalpN 104.9 52.6 81.7 69.1 75.9 62.4
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,6,4,2 Ac
3,6,4,3 Ac
3,6,4,4 Ac
3,6,4 bDGlcpN3NA 5.09 3.83 4.03 4.97 4.06 -
3,6,6,2 Ac
3,6,6 bDGlcpN 4.48 3.69 3.54 3.46 3.45 3.78-3.93
3,6 aDGalp 4.93 3.72 3.90 4.35 4.05 3.72-4.07
3 bDGlcp 4.57 3.32 3.49 3.61 3.61 3.65-3.99
2 Ac
bDGalpN 4.62 4.15 3.87 4.16 3.71 3.84
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,6,4,2 Ac
3,6,4,3 Ac
3,6,4,4 Ac
3,6,4 bDGlcpN3NA 102.5/5.09 54.5/3.83 55.8/4.03 72.0/4.97 75.2/4.06
3,6,6,2 Ac
3,6,6 bDGlcpN 103.0/4.48 56.7/3.69 75.2/3.54 71.0/3.46 76.9/3.45 61.9/3.78-3.93
3,6 aDGalp 99.2/4.93 68.5/3.72 81.7/3.90 78.1/4.35 70.6/4.05 72.3/3.72-4.07
3 bDGlcp 106.1/4.57 74.1/3.32 76.8/3.49 70.0/3.61 75.4/3.61 65.8/3.65-3.99
2 Ac
bDGalpN 104.9/4.62 52.6/4.15 81.7/3.87 69.1/4.16 75.9/3.71 62.4/3.84
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,6,4,2 | Ac | |
| 3,6,4,3 | Ac | |
| 3,6,4,4 | Ac | |
| 3,6,4 | bDGlcpN3NA | 5.09 | 3.83 | 4.03 | 4.97 | 4.06 |
|
| 3,6,6,2 | Ac | |
| 3,6,6 | bDGlcpN | 4.48 | 3.69 | 3.54 | 3.46 | 3.45 | 3.78
3.93 |
| 3,6 | aDGalp | 4.93 | 3.72 | 3.90 | 4.35 | 4.05 | 3.72
4.07 |
| 3 | bDGlcp | 4.57 | 3.32 | 3.49 | 3.61 | 3.61 | 3.65
3.99 |
| 2 | Ac | |
| | bDGalpN | 4.62 | 4.15 | 3.87 | 4.16 | 3.71 | 3.84 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,6,4,2 | Ac | |
| 3,6,4,3 | Ac | |
| 3,6,4,4 | Ac | |
| 3,6,4 | bDGlcpN3NA | 102.5 | 54.5 | 55.8 | 72.0 | 75.2 | ? |
| 3,6,6,2 | Ac | |
| 3,6,6 | bDGlcpN | 103.0 | 56.7 | 75.2 | 71.0 | 76.9 | 61.9 |
| 3,6 | aDGalp | 99.2 | 68.5 | 81.7 | 78.1 | 70.6 | 72.3 |
| 3 | bDGlcp | 106.1 | 74.1 | 76.8 | 70.0 | 75.4 | 65.8 |
| 2 | Ac | |
| | bDGalpN | 104.9 | 52.6 | 81.7 | 69.1 | 75.9 | 62.4 |
|
The spectrum also has 1 signal at unknown position (not plotted). |
There is only one chemically distinct structure:
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: nosocomial infections [ICD11:
XB25 ];
pneumonia [ICD11:
CA40 ];
bacteremia [ICD11:
MA15.0 ];
urinary tract infections (UTI) [ICD11:
GC08 ];
infection due to Acinetobacter baumannii [ICD11:
XN8LS ]
The structure was elucidated in this paperNCBI PubMed ID: 28190743Publication DOI: 10.1016/j.vaccine.2017.01.060Journal NLM ID: 8406899Publisher: Elsevier
Correspondence: shwu
gate.sinica.edu.tw
Institutions: Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan, National Health Research Institutes, Miaoli County 35053, Taiwan, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan, Department of Emergency Medicine, Taipei Veterans General Hospital, Taipei 112, Taiwan, Human Health Therapeutics, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A0R, Canada, Institute of Microbiology, Tzu Chi University, Hualien 907, Taiwan
Concerns of Acinetobacter baumannii infection have increased due to the emergence of multi-drug resistance. In the present study, we determined the capsular polysaccharide (CPS) structure of A. baumannii SK44, a clinical isolate from Taiwan, to consist of pentasaccharide repeats. We found that CPS-induced antibody provided 55% protection against challenge in an animal model. The CPS-specific antibody reacted with the surface components of about 62% clinical isolates (342/554 strains) from cross-sectional and longitudinal studies by dot-immunoassay. Pulsed-field gel electrophoresis of positive strains showed the antibody covered different clonalites of A. baumannii clinical isolates. Meanwhile, using the CPS antibody as a probe, we found a number of outer membrane proteins bound to the antibody, including OmpA/motB, TonB-dependent receptor, and Omp38, indicating their association with CPS. These results might lead to the use of the capsular polysaccharide as a vaccine to prevent A. baumannii infection.
capsular polysaccharide, Acinetobacter baumannii, Clinic population distribution, Passive immunity
Structure type: oligomer ; 1071
Location inside paper: p.1442, fig.1
Compound class: CPS
Contained glycoepitopes: IEDB_135813,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_140529,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_167069,IEDB_190606,IEDB_983931,SB_192,SB_7
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, sugar analysis, acid hydrolysis, MS/MS, serological methods, enzymatic digestion, statistical analysis, SEC, immunization, conjugation, pulsed-field gel electrophoresis (PFGE)
Comments, role: A. baumannii SK44 clinic strain; major oligosaccharide
Related record ID(s): 11986
NCBI Taxonomy refs (TaxIDs): 470Reference(s) to other database(s): GTC:G57791MT
Show glycosyltransferases
There is only one chemically distinct structure:
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