Zhang Z, Cai R, Zhang W, Fu Y, Jiao N A Novel Exopolysaccharide with Metal Adsorption Capacity Produced by a Marine Bacterium Alteromonas sp. JL2810 Marine Drugs15(6) (2017)
175
The structure was elucidated in this paper NCBI PubMed ID:28604644 Publication DOI:10.3390/md15060175 Journal NLM ID:101213729 Publisher: Basel, Switzerland: Molecular Diversity Preservation International Correspondence: zhangzilianxmu.edu.cn; jiaoxmu.edu.cn Institutions: State Key Laboratory of Marine Environmental Science, Institute of Marine Microbes and Ecospheres, Xiamen University, Xiamen 361102, China
Most marine bacteria can produce exopolysaccharides (EPS). However, very few structures of EPS produced by marine bacteria have been determined. The characterization of EPS structure is important for the elucidation of their biological functions and ecological roles. In this study, the structure of EPS produced by a marine bacterium, Alteromonas sp. JL2810, was characterized, and the biosorption of the EPS for heavy metals Cu2+, Ni2+, and Cr6+ was also investigated. Nuclear magnetic resonance (NMR) analysis indicated that the JL2810 EPS have a novel structure consisting of the repeating unit of [-3)-α-Rhap-(1→3)-α-Manp-(1→4)-α-3OAc-GalAp-(1→]. The biosorption of the EPS for heavy metals was affected by a medium pH; the maximum biosorption capacities for Cu2+ and Ni2+ were 140.8 ± 8.2 mg/g and 226.3 ± 3.3 mg/g at pH 5.0; however, for Cr6+ it was 215.2 ± 5.1 mg/g at pH 5.5. Infrared spectrometry analysis demonstrated that the groups of O-H, C=O, and C-O-C were the main function groups for the adsorption of JL2810 EPS with the heavy metals. The adsorption equilibrium of JL2810 EPS for Ni2+ was further analyzed, and the equilibrium data could be better represented by the Langmuir isotherm model. The novel EPS could be potentially used in industrial applications as a novel bio-resource for the removal of heavy metals.
exopolysaccharide, Marine bacteria, Alteromonas, metal adsorption
Zhang Z, Cai R, Zhang W, Fu Y, Jiao N A Novel Exopolysaccharide with Metal Adsorption Capacity Produced by a Marine Bacterium Alteromonas sp. JL2810 Marine Drugs15(6) (2017)
175
The structure was elucidated in this paper NCBI PubMed ID:28604644 Publication DOI:10.3390/md15060175 Journal NLM ID:101213729 Publisher: Basel, Switzerland: Molecular Diversity Preservation International Correspondence: zhangzilianxmu.edu.cn; jiaoxmu.edu.cn Institutions: State Key Laboratory of Marine Environmental Science, Institute of Marine Microbes and Ecospheres, Xiamen University, Xiamen 361102, China
Most marine bacteria can produce exopolysaccharides (EPS). However, very few structures of EPS produced by marine bacteria have been determined. The characterization of EPS structure is important for the elucidation of their biological functions and ecological roles. In this study, the structure of EPS produced by a marine bacterium, Alteromonas sp. JL2810, was characterized, and the biosorption of the EPS for heavy metals Cu2+, Ni2+, and Cr6+ was also investigated. Nuclear magnetic resonance (NMR) analysis indicated that the JL2810 EPS have a novel structure consisting of the repeating unit of [-3)-α-Rhap-(1→3)-α-Manp-(1→4)-α-3OAc-GalAp-(1→]. The biosorption of the EPS for heavy metals was affected by a medium pH; the maximum biosorption capacities for Cu2+ and Ni2+ were 140.8 ± 8.2 mg/g and 226.3 ± 3.3 mg/g at pH 5.0; however, for Cr6+ it was 215.2 ± 5.1 mg/g at pH 5.5. Infrared spectrometry analysis demonstrated that the groups of O-H, C=O, and C-O-C were the main function groups for the adsorption of JL2810 EPS with the heavy metals. The adsorption equilibrium of JL2810 EPS for Ni2+ was further analyzed, and the equilibrium data could be better represented by the Langmuir isotherm model. The novel EPS could be potentially used in industrial applications as a novel bio-resource for the removal of heavy metals.
exopolysaccharide, Marine bacteria, Alteromonas, metal adsorption
The structure was elucidated in this paper NCBI PubMed ID:31952589 Publication DOI:10.1016/j.carbpol.2019.115780 Journal NLM ID:8307156 Publisher: Elsevier Correspondence: V.S. Grinev <grinevibppm.ru> Institutions: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia, Chernyshevsky Saratov State University, 83 Ulitsa Astrakhanskaya, Saratov 410012, Russia, VIC Animal Health, VIC GROUP, 46G Berezovaya Ulitsa, Severny 1, Belgorodskaya Oblast, 8570, Russia
Paenibacillus polymyxa 92, isolated from wheat roots, produced large amounts (38.4 g L-1) of exopolysaccharide (EPS) in a liquid nutrient medium containing 10 % (w/v) sucrose. The EPS was precipitated from the culture broth with cold acetone and was purified by gel filtration and anion-exchange chromatography. The molecular mass of the EPS was 2.29-1.10 × 105 Da. Diffuse reflectance infrared Fourier transform and nuclear magnetic resonance spectra showed that the EPS was a linear β-(2→6)-linked fructan (levan). Aqueous EPS solutions showed pseudoplastic behavior when shear stress was applied at different temperatures. By using the Ostwald-de Waele model, the rheological characteristics of the EPS solution were ascertained. The sorption capacity of the EPS for Zn(II), Cd(II), Pb(II), and Cu(II) was investigated. Sorption was maximal (q = 481 mg g-1) for Cu(II) ions. In model experiments, treatment of wheat seeds with EPS solution significantly increased the length of seedling roots and shoots.
Cabrera-Barjas G, Gallardo F, Nesic A, Taboada E, Marican A, Mirabal-Gallardo Y, Avila-Salas F, Delgado N, de Armas-Ricard M, Valdes O Utilization of industrial by-product fungal biomass from Aspergillus niger and Fusarium culmorum to obtain biosorbents for removal of pesticide and metal ions from aqueous solutions Journal of Environmental Chemical Engineering8(5) (2020)
ID 104355
Publication DOI:10.1016/j.jece.2020.104355 Journal NLM ID:101673966 Publisher: Amsterdam: Elsevier Correspondence: ovaldesucm.cl Institutions: Unidad de Desarrollo Tecnológico (UDT), Universidad de Concepción, Coronel, Chile, Natural Resources Faculty, Universidad Católica de Temuco, Temuco, Chile, Miami Dade College, Miami, USA, Instituto de Química de Recursos Naturales, Universidad de Talca, Talca, Chile, Faculty of Engineering, Institute of Applied Chemistry, Universidad Autónoma de Chile, Talca, Chile, Centro de Nanotecnología Aplicada, Facultad de Ciencias, Universidad Mayor, Huechuraba, Chile, Núcleo de Química y Bioquímica, Facultad de Estudios Interdisciplinarios, Universidad Mayor, Huechuraba, Chile, Facultad de Ingeniería, Universidad Andres Bello, Talcahuano, Chile, Laboratory of Chemistry and Biochemistry, Campus Lillo, University of Aysén, Coyhaique, Chile, Vicerrectoría de Investigación y Postgrado, Universidad Católica del Maule, Talca, Chile
In this work, Aspergillus niger and Fusarium culmorum cell wall by-products were chosen as microbial sources for chitin and chitosan production. Both polysaccharides were characterized by FTIR and 13C-CPMAS NMR, but GPC analysis was only performed for chitosan. SEM-EDX analysis was performed to fungal chitosan loaded with metal ions. Chitosan extracted from both fungus had low to medium molecular weight (Mw) and degree of deacetylations (DD) ranging from 65.7-83.3%. Fungal chitosan samples were intended to be used for bioremediation applications. For this purpose, two independent absorption experiments regarding pesticide Dimethoate (DM) and heavy metal ions (Al(III), As(III), Cd(II), Cu(II), Mg(II), Mn(II), Pb(II), Zn(II), Fe(II)) in a complex mixture were carried out. An experimental design considering the solution pH, contact time and chitosan physicochemical properties (DD) were performed. The highest percentage of dimethoate pesticide and selected metal ions absorption was obtained with highest DD chitosan, the contact time of 24 h, pH 6 for metals and pH 4 for pesticide, repectively. Molecular dynamics simulation studies allowed to analyze at the molecular level the chitosan-DM interaction. A higher number of h-bonds were identified as the main interactions that stabilize the affinity of the chitosan-DM complexes. Based on our results, we suggest the use of a multipurpose fungal chitosan system for water bioremediation.
Bioremediation, Aspergillus, chitosan, Fusarium, pesticide, heavy metal
Methods: IR, biological assays, GPC, molecular modeling, extraction, cell growth, metal adsorption assays, precipitation, SEM, centrifugation, filtration, 13C CPMAS NMR Biological activity: fungal chitosan could be used as natural and versatile biosorbent to remove different water pollutants (pesticides and heavy metals)
Related record ID(s): 41553, 44877, 44886, 46311, 46570, 46683, 48760, 48774, 49133, 49502, 49512, 49524, 49653, 50016, 50301, 50303, 50304, 50307, 50310, 50311, 50314, 50315, 50317, 50319, 50320 NCBI Taxonomy refs (TaxIDs):5516, 5061 Reference(s) to other database(s): GTC:G97099AY Show glycosyltransferases
report error
Found 4 records.
Displayed records from 1 to 4
xmu.edu.cn; jiao