Taxonomic group: bacteria / Actinobacteria
(Phylum: Actinobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 28602989Publication DOI: 10.1016/j.ijbiomac.2017.06.034Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: adjuric
chem.bg.ac.rs
Institutions: University of Belgrade, Faculty of Chemistry, Studentski trg 12-16 P.O. Box 158, 11001, Belgrade, Serbia, University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Department of Chemistry, 11001 Belgrade, Njegoševa 12, Serbia, Aalto University, School of Chemical Technology, Department of Forest Products Technology Bioproduct Chemistry, Aalto, Finland, Clinical Microbiology Laboratory, Turku University Hospital, Finland
Various microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500 g/L, of sucrose. The optimal temperature and pH were 28 degrees C and 7.0, respectively. The nuclear magnetic resonance (NMR) results and GC MS analysis confirmed that the residues were D-fructofuranosyl residues with β-configuration, where fructose units are linked by β-2,6-glycosidic bonds, with β-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan.
exopolysaccharides, Polluted environments, Brachybacterium paraconglomeratum
Structure type: homopolymer
Location inside paper: p.320, fig.6
Trivial name: levan, levan-type polysaccharide
Compound class: EPS
Contained glycoepitopes: IEDB_923066
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, partial acid hydrolysis, GC-MS, DNA techniques, TLC, acid hydrolysis, GC, MALDI-TOF MS, FTIR, composition analysis, genetic methods, GPC, viscosity measurement, optical rotation measurement, reductive cleavage, dialysis, planar chromatography
Comments, role: the main chain of EPS.
Related record ID(s): 12428
NCBI Taxonomy refs (TaxIDs): 1334001Reference(s) to other database(s): GTC:G29465PK, GlycomeDB:
9593
Show glycosyltransferases
There is only one chemically distinct structure:
Taxonomic group: bacteria / Actinobacteria
(Phylum: Actinobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 28602989Publication DOI: 10.1016/j.ijbiomac.2017.06.034Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: adjuric
chem.bg.ac.rs
Institutions: University of Belgrade, Faculty of Chemistry, Studentski trg 12-16 P.O. Box 158, 11001, Belgrade, Serbia, University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Department of Chemistry, 11001 Belgrade, Njegoševa 12, Serbia, Aalto University, School of Chemical Technology, Department of Forest Products Technology Bioproduct Chemistry, Aalto, Finland, Clinical Microbiology Laboratory, Turku University Hospital, Finland
Various microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500 g/L, of sucrose. The optimal temperature and pH were 28 degrees C and 7.0, respectively. The nuclear magnetic resonance (NMR) results and GC MS analysis confirmed that the residues were D-fructofuranosyl residues with β-configuration, where fructose units are linked by β-2,6-glycosidic bonds, with β-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan.
exopolysaccharides, Polluted environments, Brachybacterium paraconglomeratum
Structure type: polymer biological repeating unit
Location inside paper: abstract, p.317, p.320, fig.6
Trivial name: levan-type polysaccharide
Compound class: EPS
Contained glycoepitopes: IEDB_923066
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, partial acid hydrolysis, GC-MS, DNA techniques, TLC, acid hydrolysis, GC, MALDI-TOF MS, FTIR, composition analysis, genetic methods, GPC, viscosity measurement, optical rotation measurement, reductive cleavage, dialysis, planar chromatography
Comments, role: proposed of structure of the EPS. The structure in Fig. 6 is depicted erroneously, see text.
Related record ID(s): 12036
NCBI Taxonomy refs (TaxIDs): 1334001Reference(s) to other database(s): GTC:G66135XB
Show glycosyltransferases
NMR conditions: in D2O at 298 K
1H NMR data: present in publication
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13C NMR data: present in publication
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There is only one chemically distinct structure:
Found 
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