Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Pseudomonas [ICD11:
XN022 
]
The structure was elucidated in this paperNCBI PubMed ID: 28526845Publication DOI: 10.1038/s41598-017-02145-wJournal NLM ID: 101563288Publisher: London: Nature Publishing Group
Correspondence: yklee
kopri.re.kr; seokor
konkuk.ac.kr; ygkim
ssu.ac.kr
Institutions: Department of Chemical Engineering, Soongsil University, Seoul, 06978, Korea, Department of Biological Engineering, Konkuk University, Seoul, 05029, Korea, Division of Bioconvergence Analysis, Korea Basic Science Institute, Chungbuk, 34133, Korea, Division of Life Sciences, Korea Polar Research Institute, Incheon, 21990, Korea
Climate change causes permafrost thawing, and we are confronted with the unpredictable risk of newly discovered permafrost microbes that have disease-causing capabilities. Here, we first characterized the detailed chemical structure of the lipid A moiety from a Pseudomonas species that was isolated from thawing arctic permafrost using MALDI-based mass spectrometric approaches (i.e., MALDI-TOF MS and MALDI-QIT-TOF MSn). The MALDI multi-stage mass spectrometry (MS) analysis of lipid A extracted from the Pseudomonas sp. strain PAMC 28618 demonstrated that the hexaacyl lipid A ([M-H]- at m/z 1616.5) contains a glucosamine (GlcN) disaccharide backbone, two phosphates, four main acyl chains and two branched acyl chains. Moreover, the lipid A molecule-based structural activity relationship with other terrestrial Gram-negative bacteria indicated that strain PAMC 28618 has an identical lipid A structure with the mesophilic Pseudomonas cichorii which can cause rot disease in endive (Cichorium endivia) and that their bacterial toxicities were equivalent. Therefore, the overall lipid A validation process provides a general strategy for characterizing bacteria that have been isolated from arctic permafrost and analyzing their respective pathogenicities.
Lipopolysaccharide, lipid A, MALDI-TOF, cytokine induction, Pseudomonas cichorii, Pseudomonas sp. strain PAMC 28618
Structure type: oligomer ; 1616.5 [M-H]-
Location inside paper: fig.3(C)
Compound class: lipid A
Contained glycoepitopes: IEDB_135394,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_534864
Methods: PCR, de-O-acylation, DNA techniques, acid hydrolysis, MALDI-TOF MS, composition analysis, extraction, phylogenetic analysis, MALDI-QIT-TOF MS
NCBI Taxonomy refs (TaxIDs): 306,
36746
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error