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1. (Article ID: 12088)
 
Choi JS, Kim JE, Kim JI, Cheigh HS, Yokozawa T
A flavonol diglucoside from the leaves of Brassica juncea
Natural Product Sciences 6(4) (2000) 199-200
 

A flavonol diglucoside was isolated from the leaves of Brassica juncea L. The structure of the compound was elucidated as isorhamnetin 3,7-di-O-β-D-glucopyranoside (1) on the basis of chemical and spectral evidence.

Brassica juncea, Brassicaceae, flavonol diglucoside, isorhamnetin 3, 7-di-O-β-D-glucopyranoside

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2. (Article ID: 12356)
 
Leoni O, Iori R, Palmieri S
Hydrolysis of glucosinolates using nylon-immobilized myrosinase to produce pure bioactive molecules
Biotechnology and Bioengineering 68(6) (2000) 660-664
 

Bioactive compounds were produced from natural glucosinolates, secondary plant metabolites, using myrosinase (thioglucoside glucohydrolase EC 3.2.3.1) isolated from ripe seeds of Sinapis alba. The enzyme was immobilized on granular nylon 6.6 with the crosslinking technique. Immobilized myrosinase displayed extraordinary operational and storage stability. Using a small thermostatted continuous packed-bed bioreactor, the enzyme activity was unchanged after 15 days of continuous use at 37°C and after >1 year of storage at room temperature. The bioreactor was particularly efficient in producing pure isothiocyanates, but it was less efficient for pure nitrile production.

immobilization, bioreactor, myrosinase, glucosinolates, isothiocyanates, nitriles

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