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The O-specific polysaccharide was obtained from the lipopolysaccharide of the legume-endosymbiotic bacterium Ochrobactrum cytisi strain ESC1(T) and studied by chemical analyses and 1D and 2D NMR spectroscopy. The polysaccharide was found to have a disaccharide repeating unit containing α-d-fucose and β-N-acetyl-d-galactosamine residues connected via (1→3)-glycosidic bonds, resulting in the following structure: →3)-α-d-Fucp-(1→3)-β-d-GalpNAc-(1→ The d-GalpNAc residue was nonstoichiometrically substituted with a 4-O-methyl group (approximately 10%) or with a 4,6-O-(1-carboxy)-ethylidene residue (pyruvyl group) (approximately 10%).

O-specific polysaccharide, fucose, galactosamine, lipopolysaccharid, Ochrobactrum cytisi, pyruvyl

Publication DOI: 10.1016/j.carres.2015.05.002
Journal NLM ID: 0043535
Publisher: Elsevier
Correspondence: adam.choma@poczta.umcs.lublin.pl
Institutions: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, SDS-PAGE, acid hydrolysis, composition analysis, NMR-1D, GPC

The O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide of Aeromonas veronii strain Bs8, which is pathogenic to common carp (Cyprinus carpio), after mild acid hydrolysis followed by gel-permeation chromatography. The high-molecular-mass OPS fraction was investigated using chemical methods, mass spectrometry, and 1H and 13C NMR spectroscopy techniques, including 2D homonuclear 1H,1H TOCSY, DQF COSY, NOESY, and heteronuclear 1H-detected 1H,13C HSQC, and HMBC experiments. The analysis revealed that the O-specific polysaccharide contains sugars with the galacto configuration of the ring and is composed of a disaccharide repeating unit with the following structure. (formula: see text).

LPS, O-antigen, O-specific polysaccharide, Aeromonas veronii, Fish pathogen, D-fucopyranose, D-Fucp, GalpNAc

NCBI PubMed ID: 33298315
Publication DOI: 10.1016/j.carres.2020.108210
Journal NLM ID: 0043535
Publisher: Elsevier
Correspondence: aturska@hektor.umcs.lublin.pl
Institutions: Department of Fish Diseases, National Veterinary Research Institute, Partyzantów 57, 24-100 Pulawy, Poland, Department of Genetics and Microbiology, Institute of Microbiology and Biotechnology, M. Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, SDS-PAGE, chemical analysis, mild acid hydrolysis, GPC

Presented herein is the synthesis of the Aeromonas veronii disaccharide repeating unit which has been achieved in 11 steps starting from D-fucose and D-galactosamine.

synthesis, Gram-negative, fucose, glycosylation, GalNAc

NCBI PubMed ID: 35263695
Publication DOI: 10.1016/j.carres.2022.108530
Journal NLM ID: 0043535
Publisher: Elsevier
Correspondence: S.D. Townsend
Institutions: Department of Chemistry, Vanderbilt University, Nashville, TN, 37235, United States
Methods: 13C NMR, 1H NMR, NMR-2D, IR, TLC, chemical synthesis, glycosylation, optical rotation measurement, SEC, HR-ESI-MS

Presented herein is the synthesis of the Aeromonas veronii disaccharide repeating unit which has been achieved in 11 steps starting from D-fucose and D-galactosamine.

synthesis, Gram-negative, fucose, glycosylation, GalNAc