Salimath PV, Tharanathan RN, Weckesser J, Mayer H The structure of the polysaccharide moiety of Rhodopseudomonas sphaeroides ATCC 17023 lipopolysaccharide European Journal of Biochemistry144 (1984)
227-232
The structure was elucidated in this paper NCBI PubMed ID:6333338 Publication DOI:10.1111/j.1432-1033.1984.tb08454.x Journal NLM ID:0107600 Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies Institutions: Max-Planck-Institut für Immunbiologie, Institut für Biologie II, Mikrobiologie, Freiburg, Germany
The chemical structure of the polysaccharide moiety of the lipopolysaccharide Rhodopseudomonas sphaeroides ATCC 17023 was established. Mild acetic acid hydrolysis of isolated lipopolysaccharide, followed by preparative high-voltage paper electrophoresis afforded three oligosaccharides. They were characterized by chemical and physicochemical studies to be: GlcA(α1→4)dOclA8P, Thr(6') GlcA(α1→4)GlcA and GlcA(α1→4)dOclA, where GlcA is D-glucuronic acid and dOc1A is 3-deoxy-D-manno-octulosonic acid. Carboxyl-reduction of the lipopolysaccharide followed by acid hydrolysis gave a trisaccharide: GlcA(α1→4)Glc(α1→4)Glc, showing the presence of three residues of glucuronic acids in the O-specific chain and indicating that only two of them are reducible by NaBH4. The linkage between the polysaccharide and lipid A was shown to be through a single 1,4-linked residue of dOc1A attached by a 2,6'-linkage to the lipid A moiety.
Related record ID(s): 122847 NCBI Taxonomy refs (TaxIDs):272943, 1073 Reference(s) to other database(s): GTC:G68305MS, CCSD:10747, CBank-STR:754 Show glycosyltransferases
There are 3 chemically distinct structures. Please, select:
Salimath PV, Tharanathan RN, Weckesser J, Mayer H The structure of the polysaccharide moiety of Rhodopseudomonas sphaeroides ATCC 17023 lipopolysaccharide European Journal of Biochemistry144 (1984)
227-232
The structure was elucidated in this paper NCBI PubMed ID:6333338 Publication DOI:10.1111/j.1432-1033.1984.tb08454.x Journal NLM ID:0107600 Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies Institutions: Max-Planck-Institut für Immunbiologie, Institut für Biologie II, Mikrobiologie, Freiburg, Germany
The chemical structure of the polysaccharide moiety of the lipopolysaccharide Rhodopseudomonas sphaeroides ATCC 17023 was established. Mild acetic acid hydrolysis of isolated lipopolysaccharide, followed by preparative high-voltage paper electrophoresis afforded three oligosaccharides. They were characterized by chemical and physicochemical studies to be: GlcA(α1→4)dOclA8P, Thr(6') GlcA(α1→4)GlcA and GlcA(α1→4)dOclA, where GlcA is D-glucuronic acid and dOc1A is 3-deoxy-D-manno-octulosonic acid. Carboxyl-reduction of the lipopolysaccharide followed by acid hydrolysis gave a trisaccharide: GlcA(α1→4)Glc(α1→4)Glc, showing the presence of three residues of glucuronic acids in the O-specific chain and indicating that only two of them are reducible by NaBH4. The linkage between the polysaccharide and lipid A was shown to be through a single 1,4-linked residue of dOc1A attached by a 2,6'-linkage to the lipid A moiety.
Related record ID(s): 122846 NCBI Taxonomy refs (TaxIDs):272943, 1073 Reference(s) to other database(s): GTC:G03216VO, CCSD:10748, CBank-STR:1595 Show glycosyltransferases
There are 3 chemically distinct structures. Please, select: