Initial hydridization tests using Aeromonas salmonicida typical and atypical strains showed the possibility of different lipopolysaccharide (LPS) outer cores among these strains. By chemical structural analysis, LPS-core SDS-PAGE gel migration, and functional and comparative genomics we demonstrated that typical A. salmonicida (subsp. salmonicida) strains and atypical subsp. masoucida and probably smithia strains showed the same LPS outer core. A. salmonicida subsp. achromogenes strains show a similar LPS outer core but lack one of the most external residues (a galactose linked α1-6 to heptose), not affecting the O-antigen LPS linkage. A. salmonicida subsp. pectinolytica strains show a rather changed LPS outer core, which is identical to the LPS outer core from the majority of the A. hydrophila strains studied by genomic analyses. The LPS inner core in all tested A. salmonicida strains, typical and atypical, is well-conserved. Furthermore, the LPS inner core seems to be conserved in all the Aeromonas (psychrophilic or mesophilic) strains studied by genomic analyses.
lipopolysaccharide core, genomics, Aeromonas salmonicida, inner and outer core chemical structure, typical and atypical
NCBI PubMed ID: 26904002Publication DOI: 10.3389/fmicb.2016.00125Journal NLM ID: 101548977Publisher: Lausanne: Frontiers Research Foundation
Correspondence: jtomas@ub.edu (Juan M. Tomas)
Institutions: Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona Barcelona, Spain
Methods: methylation, PCR, SDS-PAGE, sugar analysis, DNA techniques, GLC, MALDI-TOF MS, genetic methods, bioinformatic analysis, SDS-Tricine-PAGE
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