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1. (Article ID: 12356)
Leoni O, Iori R, Palmieri S
Hydrolysis of glucosinolates using nylon-immobilized myrosinase to produce pure bioactive molecules
Biotechnology and Bioengineering 68(6) (2000)
660-664
Bioactive compounds were produced from natural glucosinolates, secondary plant metabolites, using myrosinase (thioglucoside glucohydrolase EC 3.2.3.1) isolated from ripe seeds of Sinapis alba. The enzyme was immobilized on granular nylon 6.6 with the crosslinking technique. Immobilized myrosinase displayed extraordinary operational and storage stability. Using a small thermostatted continuous packed-bed bioreactor, the enzyme activity was unchanged after 15 days of continuous use at 37°C and after >1 year of storage at room temperature. The bioreactor was particularly efficient in producing pure isothiocyanates, but it was less efficient for pure nitrile production.
immobilization, bioreactor, myrosinase, glucosinolates, isothiocyanates, nitriles
The publication contains the following compound(s):
- Compound ID: 32061
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b-D-Glcp1S-(1-2)-Subst
Subst = (1E,4E)-5-(methylsulfinyl)-N-(sulfooxy)pent-4-enimidothioic acid = SMILES S/{2}C(CC/C=C/S(C)=O)=N/OS(=O)(O)=O |
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Structure type: monomer
Trivial name: glucoraphenin, glucoraphanin
Compound class: glycoside, glucosinolate
- Compound ID: 32059
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b-D-Glcp1S-(1-2)-Subst
Subst = (E)-2-(4-hydroxyphenyl)-N-(sulfooxy)ethanimidothioic acid = SMILES S/{2}C(CC1=CC(O)=CC=C1)=N/OS(=O)(O)=O |
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Structure type: monomer
Trivial name: sinalbin
Compound class: glycoside, glucosinolate
- Compound ID: 31348
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b-D-Glcp1S-(1-2)-Subst
Subst = (E)-N-(sulfooxy)but-3-enimidothioic acid = SMILES C=CC/{2}C(S)=N\OS(=O)(O)=O |
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Structure type: monomer
Trivial name: sinigrin
Compound class: glycoside, glucosinolate
- Compound ID: 32056
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b-D-Glcp1S-(1-2)-Subst
Subst = (E)-N-(sulfooxy)pent-4-enimidothioic acid = SMILES C=CCC/{2}C(S)=N\OS(=O)(O)=O |
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Structure type: monomer
Trivial name: gluconapin
Compound class: glycoside, glucosinolate
- Compound ID: 32057
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b-D-Glcp1S-(1-2)-Subst
Subst = (E)-3-hydroxy-N-(sulfooxy)pent-4-enimidothioic acid = SMILES C=CC(O)C/{2}C(S)=N\OS(=O)(O)=O |
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Structure type: monomer
Trivial name: epi-progoitrin, epiprogoitrin, progoitrin
Compound class: glycoside, glucosinolate
- Compound ID: 32058
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b-D-Glcp1S-(1-2)-Subst
Subst = (E)-2-phenyl-N-(sulfooxy)ethanimidothioic acid = SMILES S/{2}C(CC1=CC=CC=C1)=N/OS(=O)(O)=O |
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Structure type: monomer
Trivial name: glucotropaeolin
Compound class: glycoside, glucosinolate
- Compound ID: 32060
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b-D-Glcp1S-(1-2)-Subst
Subst = (E)-4-(methylthio)-N-(sulfooxy)butanimidothioic acid = SMILES S/{2}C(CCCSC)=N/OS(=O)(O)=O |
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Structure type: monomer
Trivial name: glucoerucin
Compound class: glycoside, glucosinolate
- Compound ID: 32062
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b-D-Glcp1S-(1-2)-Subst
Subst = (E)-4-(methylsulfonyl)-N-(sulfooxy)butanimidothioic acid = SMILES S/{2}C(CCCS(C)(=O)=O)=N/OS(=O)(O)=O |
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Structure type: monomer
Trivial name: glucocheirolin
Compound class: glycoside, glucosinolate
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