Capsular polysaccharides were recovered from four Acinetobacter baumannii isolates, and the following related structures of oligosaccharide repeating units were established by sugar analyses along with 1D and 2D 1H and 13C NMR spectroscopy: NIPH 60 and LUH5544 (K43) NIPH 601 (K47) The K locus for capsule biosynthesis in the genome sequences available for NIPH 60 and LUH5544, designated KL43, was found to be related to gene clusters KL47 in NIPH 601 and KL88 in LUH5548. The three clusters share most gene content differing in only a small portion that includes an additional glycosyltransferase genes in KL47 and KL88, as well as genes encoding distinct Wzy polymerases that were found to form the same α-d-GlcpNAc-(1→6)-α-d-GlcpNAc linkage in K43 and K47.
capsular polysaccharide, Acinetobacter baumannii, glycosyltransferase, Wzy polymerase, K locus
Publication DOI: 10.1016/j.carres.2016.10.007Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: yknirel@gmail.com
Institutions: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia, School of Molecular Bioscience, The University of Sydney, Sydney, Australia, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia, M.M. Shemyakin & Y.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russi, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia, School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, chemical analysis, acid hydrolysis, GLC, NMR-1D, bioinformatic analysis
Found