Haard K, Bitter W, Kamerling JP, Vliegenthart JFG O-Mannosylation of recombinant human insulin-like growth factor I (IGF-I) produced in Saccharomyces cerevisiae FEBS Letters248 (1989)
111-114
The structure was elucidated in this paper NCBI PubMed ID:2656293 Journal NLM ID:0155157 Publisher: Elsevier Institutions: Department of Bio-Organic Chemistry, Utrecht University, Utrecht, The Netherlands
A glycosylated form of recombinant human insulin-like growth factor I (IGF-I) expressed in Saccharomyces cerevisiae was shown to contain mannose as the only carbohydrate constituent. All oligosaccharide chains of the glycoprotein could be released by mild alkaline treatment, and separated from the protein by gel-permeation chromatography on Bio-Gel P-2. The structures of these O-linked carbohydrate chains were determined by 500-MHz 1H-NMR spectroscopy, affording the disaccharide Man α 1-2Man as the major component and the tetrasaccharide Man α 1-3Man α 1-2Man α 1-2Man as a minor component. Reference oligosaccharides were prepared from mannoproteins released from the cell wall of S. cerevisiae X2180 (alpha-wild type). In addition to previously reported structures, ranging from mannose to mannotetraose, the pentasaccharide Man α 1-3Man α 1-3Man α 1-2Man α 1-2Man was identified in the cell wall mannoprotein.
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