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1. (Article ID: 12621)
 
Romani A, Pinelli P, Mulinacci N, Vincieri FF, Gravano E, Tattini M
HPLC analysis of flavonoids and secoiridoids in leaves of Ligustrum vulgare L. (Oleaceae)
Journal of Agricultural and Food Chemistry 48(9) (2000) 4091-4096
 

Identification and quantification of flavonol glycosides and secoiridoids was carried out on leaves of Ligustrum vulgare L. (Oleaceae) by means of HPLC-DAD and HPLC-MS analysis. In addition to previously reported secoiridoids (oleuropein, ligustaloside A, ligustaloside B, and ligstroside) four kaempferol glycosides (kaempferol 3-O-glucoside 7-O-rhamnoside, kaempferol 3, 7-O-dirhamnoside, kaempferol 3-O-rhamnoside, and kaempferol 3-O-glucoside) and two quercetin glycosides (quercetin 3-O-glucoside 7-O-rhamnoside and quercetin 3,7-O-dirhamnoside) were present in leaves of L. vulgare L. Although secoiridoids accounted for nearly the 76% of the total leaf polyphenols content (with ligustaloside A as the main component), kaempferol glycosides were also accumulated in the leaves of L. vulgare L. to a relatively high extent (23%). Contribution of quercetin derivatives was minor under our experimental conditions. Our findings suggest that flavonol glycosides may have a central role in both the ecology and the biology of L. vulgare L.

HPLC-DAD; HPLC-MS; kaempferol glycosides; privet; quercetin glycosides; oleuropein; flavonoids

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