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Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130422,IEDB_135813,IEDB_135849,IEDB_136105,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_225177,IEDB_885823

• Article ID: 5031
  Pakiet K, Turska-Szewczuk A, Karaś MA, Pekala A, Pietras H "Structure of the O-specific polysaccharide from the lipopolysaccharide of Aeromonas hydrophila strain K691 containing 4-acetamido-4,6-dideoxy-D-glucose" - Carbohydrate Research 439 (2017) 23-29
  

  The O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide of Aeromonas hydrophila strain K691 and studied by chemical methods and 1H and 13C NMR spectroscopy, including 2D 1H,1H COSY, TOCSY, NOESY, 1H-detected heteronuclear 1H,13C HSQC, and HMBC experiments. It was found that the O-specific polysaccharide was built up of pentasaccharide repeating units composed of β-GlcpNAc, 2-O-acetylated α-Rhap, and β-Quip4NAc residues. The following structure of the OPS was established: →3)-α-l-Rha2OAc-(1→3)-β-d-GlcNAc-(1→3)-α-l-Rha2OAc-(1→3)-β- d-GlcNAc-(1→2)-β-d-Qui4NAc-(1→.

  LPS, O-antigen, O-specific polysaccharide, O-acetylation, 6-dideoxy-d-glucose, 4-amino-4, Aeromonas hydrophila, Fish pathogen

  NCBI PubMed ID: 28088128
  Publication DOI: 10.1016/j.carres.2016.12.006
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: aturska@hektor.umcs.lublin.pl
  Institutions: Department of Genetics and Microbiology, M. Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland, Department of Fish Diseases, National Veterinary Research Institute, Partyzantów 57, 24-100 Pulawy, Poland
  Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, SDS-PAGE, sugar analysis, acid hydrolysis, GPC, reduction with NaBD4, N-acetylation
  
   
  
  Aeromonas hydrophila K691
  CSDB ID 11947 (all data & tools)

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Structure type: oligomer
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_120354,IEDB_135813,IEDB_135849,IEDB_136105,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_225177,IEDB_885823

• Article ID: 5393
  Adak A, Mukhopadhyay B "Chemical synthesis of the 4-amino-4,6-dideoxy-D-glucose containing pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophila strain K691 in the form of its 2-aminoethyl glycoside" - Carbohydrate Research 476(1) (2019) 1-7
  

  Chemical synthesis of the pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophilastrain K691 is reported. Synthesis of the pentasaccharide is accomplished by using a common disaccharide in sequence and finally attaching the rare sugar unit. The target structure was made in the form of its 2-aminoethyl glycoside which is essential for further glycconjugate formation. Stereoselective glycosylations were achieved by the activation of thioglycosides in the presence of H2SO4-silica in conjunction with N-iodosuccinimide.

  O-specific polysaccharide, 6-dideoxy-d-glucose, 4-amino-4, total synthesis, Fish pathogen, H(2)SO(4)-Silica

  NCBI PubMed ID: 30861377
  Publication DOI: 10.1016/j.carres.2019.02.009
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: B. Mukhopadhyay
  Institutions: Sweet Lab, Department of Chemical Sciences, Indian Institute of Science Education and Research (IISER) Kolkata, Mohanpur, Nadia, 741246, India
  Methods: 13C NMR, 1H NMR, TLC, chemical synthesis, optical rotation measurement, flash chromatography
  
   
  
  Aeromonas hydrophila K691
  CSDB ID 953 (all data & tools)