Novel teichulosonic acid with the repeating unit →6)-β-D-GlcpNAc-(1→8)-α-Kdn-(2→ has been found in the cell walls of two Arthrobacter strains, VKM Ac-2549 and VKM Ac-2550. The teichulosonic acid was revealed in representatives of the genus Arthrobacter for the first time. Two other polymers identified in the above strains were poly(monoglycosyl 1-phosphate) and poly(diglycosyl 1-phosphate) of hitherto unknown structures, i.e., -6)-α-D-GalpNAc-(1-P-, and -6)-β-D-GlcpNAc-(1→3)-α-D-Galp-(1-P-. The structures of all three polymers were established by using chemical, NMR spectroscopic and ESI-MS methods. The strains studied in this work differ in the cell wall composition from the type strain of phylogenetically closely related species A. crystallopoietes which was reported to contain a teichoic acid and supposedly had a glycosyl 1-phosphate polymer.
Kdn, Arthrobacter, teichulosonic acid, glycosyl 1-phosphate polymer, Cell wall glycopolymer
NCBI PubMed ID: 28942028Publication DOI: 10.1016/j.carres.2017.09.004Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: potekhina56@mail.ru
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, School of Biology, M. V. Lomonosov Moscow State University, Moscow, Russian Federation, All-Russian Collection of Microorganisms (VKM), G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russia
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, 31P NMR, ESI-MS, acid hydrolysis, GLC, paper chromatography, electrophoresis, ion-exchange chromatography
Found