Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 30241821Publication DOI: 10.1016/j.carbpol.2018.08.072Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: gerardino.derrico
unina.it; silipo
unina.it
Institutions: Dipartimento di Scienze Chimiche, Complesso Universitario Monte Sant'Angelo, Universita di Napoli Federico II, Via Cintia 4, I-80126 Napoli, Italy, IRD, Laboratoire des Symbioses Tropicales et Mediterraneennes (LSTM), UMR IRD/ SupAgro/INRA/ UM2 /CIRAD, TA-A82/J - Campus de Baillarguet, 34398 Montpellier Cedex 5, France
Bacterial cell surface exopolysaccharides (EPS) provide a protective barrier from the external milieu and participate in host-environment interactions. Zymomonas mobilis, an ethanologenic Gram negative bacterium, is used by the industry in bio-ethanol production process, due to its extraordinary resistance to a highly ethanolic environment. We found that Z. mobilis produces a mixture of two EPSs, an [α-(1→6)-D-Manp] mannose homopolymer and a galactose containing polysaccharide: [→2)-β-D-Galf-(1→3)-β-D-Galp-(1→]n. A physico-chemical study, conducted with diffusion-ordered spectroscopy (DOSY) and Dynamic Light Scattering (DLS), allowed to demonstrate that, differently from the majority of polysaccharides, ethanol is a good solvent for the galactose containing EPS, revealing that its chemical structure is specifically connected with the Zymomonas mobilis high ethanol tolerance.
NMR spectroscopy, exopolysaccharide, DLS, DOSY, Ethanologenic bacteria, Zymomonas mobilis
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.295, fig.1, p.296, table 1a, PS1
Compound class: EPS, O-polysaccharide, O-antigen, CPS
Contained glycoepitopes: IEDB_136044,IEDB_136095,IEDB_137472,IEDB_141794,IEDB_190606,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, SDS-PAGE, composition analysis, dynamic light scattering (DLS), DOSY
Related record ID(s): 12958
NCBI Taxonomy refs (TaxIDs): 542Reference(s) to other database(s): GTC:G44511KG, GlycomeDB:
3496
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3 bDGalf 108.2 88.9 75.9 82.6 70.5 62.5
bDGalp 101.9 69.6 79.8 68.4 74.9 60.5
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3 bDGalf 5.34 4.29 4.14 3.96 3.74 3.55
bDGalp 4.54 3.52 3.66 3.97 3.62 3.64
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3 bDGalf 108.2/5.34 88.9/4.29 75.9/4.14 82.6/3.96 70.5/3.74 62.5/3.55
bDGalp 101.9/4.54 69.6/3.52 79.8/3.66 68.4/3.97 74.9/3.62 60.5/3.64
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3 | bDGalf | 5.34 | 4.29 | 4.14 | 3.96 | 3.74 | 3.55 |
| | bDGalp | 4.54 | 3.52 | 3.66 | 3.97 | 3.62 | 3.64 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3 | bDGalf | 108.2 | 88.9 | 75.9 | 82.6 | 70.5 | 62.5 |
| | bDGalp | 101.9 | 69.6 | 79.8 | 68.4 | 74.9 | 60.5 |
|
There is only one chemically distinct structure:
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 30241821Publication DOI: 10.1016/j.carbpol.2018.08.072Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: gerardino.derrico
unina.it; silipo
unina.it
Institutions: Dipartimento di Scienze Chimiche, Complesso Universitario Monte Sant'Angelo, Universita di Napoli Federico II, Via Cintia 4, I-80126 Napoli, Italy, IRD, Laboratoire des Symbioses Tropicales et Mediterraneennes (LSTM), UMR IRD/ SupAgro/INRA/ UM2 /CIRAD, TA-A82/J - Campus de Baillarguet, 34398 Montpellier Cedex 5, France
Bacterial cell surface exopolysaccharides (EPS) provide a protective barrier from the external milieu and participate in host-environment interactions. Zymomonas mobilis, an ethanologenic Gram negative bacterium, is used by the industry in bio-ethanol production process, due to its extraordinary resistance to a highly ethanolic environment. We found that Z. mobilis produces a mixture of two EPSs, an [α-(1→6)-D-Manp] mannose homopolymer and a galactose containing polysaccharide: [→2)-β-D-Galf-(1→3)-β-D-Galp-(1→]n. A physico-chemical study, conducted with diffusion-ordered spectroscopy (DOSY) and Dynamic Light Scattering (DLS), allowed to demonstrate that, differently from the majority of polysaccharides, ethanol is a good solvent for the galactose containing EPS, revealing that its chemical structure is specifically connected with the Zymomonas mobilis high ethanol tolerance.
NMR spectroscopy, exopolysaccharide, DLS, DOSY, Ethanologenic bacteria, Zymomonas mobilis
Structure type: homopolymer
Location inside paper: abstract, p.295, fig.1, p.297, table 1b, PS2
Trivial name: LAM, mannan, galactomannan, cell wall heteromannan
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, N-glycan, cell surface polysaccharide, mannan, D-mannan, galactomannan
Contained glycoepitopes: IEDB_130701,IEDB_140116,IEDB_141793,IEDB_141828,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_153762,IEDB_153763,IEDB_76933,IEDB_983930,SB_198,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, SDS-PAGE, composition analysis, dynamic light scattering (DLS), DOSY
Related record ID(s): 12753
NCBI Taxonomy refs (TaxIDs): 542Reference(s) to other database(s): GTC:G55317BB, GlycomeDB:
6776, CCSD:
46066, CBank-STR:6345
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
aDManp 99.3 69.9 70.8 66.4 70.6 65.4
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
aDManp 4.80 3.89 3.73 3.61 3.74 3.68-3.92
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
aDManp 99.3/4.80 69.9/3.89 70.8/3.73 66.4/3.61 70.6/3.74 65.4/3.68-3.92
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | aDManp | 4.80 | 3.89 | 3.73 | 3.61 | 3.74 | 3.68
3.92 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | aDManp | 99.3 | 69.9 | 70.8 | 66.4 | 70.6 | 65.4 |
|
There is only one chemically distinct structure:
Found 
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