Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Haemophilus influenzae [ICD11:
XN1P6 
]
NCBI PubMed ID: 9245443Journal NLM ID: 0370535Publisher: Academic Press
Institutions: Research Department, North American Vaccine, Inc., Beltsville, Maryland 20705
Two methods are described for direct molar-mass measurement of low-molar-mass fragments obtained by oxidative cleavage of the capsular polysaccharide of Haemophilus influenzae type b. Absolute molar masses were determined by size-exclusion chromatography (SEC) with detection by multiangle laser-light-scattering photometry (MALLS) and differential refractometry (RI). The end-group structure of the polysaccharide fragments allowed the direct measurement of average chain length by quantitative 1H NMR, from which molar masses were derived. Variation between the molar masses obtained by the two methods ranged from 5 to 7%. When molar masses estimated by indirect methods were compared to SEC-MALLS/RI data, significant deviations were observed. Analysis by SEC with secondary calibration with dextran standards gave molar masses that exceeded the SEC-MALLS/RI data by as much as 2.5-fold. Molar masses estimated by a combination of colorimetric assays varied from the SEC-MALLS/RI data by as much as 50%. These results demonstrated the applicability and superior accuracy of the direct methods of molar-mass determination of the polysaccharide fragments.
NMR, Haemophilus, Haemophilus influenzae, structure, chemistry, variation, capsular, polysaccharide, analysis, capsular polysaccharide, chain, type, Magnetic Resonance Spectroscopy, method, fragment, methods, chromatography, measurement, quantitative, Bacterial Capsules, Haemophilus influenzae type b, detection, 1H NMR, chain length, cleavage, colorimetry, oxidative
Structure type: polymer chemical repeating unit ; 10000
Location inside paper: page 228
Compound class: CPS
Contained glycoepitopes: IEDB_114703,IEDB_146102,IEDB_146103,IEDB_149136,IEDB_591403,IEDB_910421
Methods: NMR, MS, colorimetry, oxidative partial depolymerization
NCBI Taxonomy refs (TaxIDs): 727Reference(s) to other database(s): GTC:G65739XY, GlycomeDB:
16923
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There is only one chemically distinct structure:
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