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1. (CSDB ID: 12782) | report error |
| 3HOMyr-(1-3)-+ | b-L-Arap4N-(1--P--1)--+ | | | 3HOMyr-(1-3)-+ | | | | | 3HOPam-(1-2)-b-D-GlcpN-(1-6)-a-D-GlcpN | 3HOPam-(1-2)-+ | Show graphically |
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Burkholderia pseudomallei K96243
(NCBI TaxID 272560,
species name lookup)
, ICD11: XN3LD ]; infection due to Burkholderia pseudomallei [ICD11: XN3LD ]
tropmedres.acBurkholderia pseudomallei is an environmental bacterium that causes melioidosis, a major community-acquired infection in tropical regions. Melioidosis presents with a range of clinical symptoms, is often characterized by a robust inflammatory response, may relapse after treatment, and results in high mortality rates. Lipopolysaccharide (LPS) of B. pseudomallei is a potent immunostimulatory molecule comprised of lipid A, core, and O-polysaccharide (OPS) components. Four B. pseudomallei LPS types have been described based on SDS-PAGE patterns that represent the difference of OPS-type A, type B, type B2 and rough LPS. The majority of B. pseudomallei isolates are type A. We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) followed by electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QqTOF MS) and gas chromatography to characterize the lipid A of B. pseudomallei within LPS type A isolates. We determined that B. pseudomallei lipid A is represented by penta- and tetra-acylated species modified with 4-amino-4-deoxy-arabinose (Ara4N). The MALDI-TOF profiles from 171 clinical B. pseudomallei isolates, including 68 paired primary and relapse isolates and 35 within-host isolates were similar. We did not observe lipid A structural changes when the bacteria were cultured in different growth conditions. Dose-dependent NF-kappa B activation in HEK cells expressing TLR4 was observed using multiple heat-killed B. pseudomallei isolates and corresponding purified LPS. We demonstrated that TLR4-dependent NF-kappa B activation induced by heat-killed bacteria or LPS prepared from OPS deficient mutant was significantly greater than those induced by wild type B. pseudomallei. These findings suggest that the structure of B. pseudomallei lipid A is highly conserved in a wide variety of clinical and environmental circumstances but that the presence of OPS may modulate LPS-driven innate immune responses in melioidosis.
structure, lipid A, Burkholderia pseudomallei, immune response, melioidosis, TLR4
Structure type: oligomer ; 1511|
2. (CSDB ID: 12783) | report error |
| 3HOMyr-(1-3)-+ | b-L-Arap4N-(1--P--1)--+ | | | ?%b-L-Arap4N-(1--P--4)--+ | | | | | 2HOMyr-(1-3)-3HOPam-(1-2)-b-D-GlcpN-(1-6)-a-D-GlcpN | | 3HOMyr-(1-3)-+ 3HOPam-(1-2)-+ | Show graphically |
|
Show legend Show as text |
Burkholderia pseudomallei K96243
(NCBI TaxID 272560,
species name lookup)
, ICD11: XN3LD ]; infection due to Burkholderia pseudomallei [ICD11: XN3LD ]tropmedres.acBurkholderia pseudomallei is an environmental bacterium that causes melioidosis, a major community-acquired infection in tropical regions. Melioidosis presents with a range of clinical symptoms, is often characterized by a robust inflammatory response, may relapse after treatment, and results in high mortality rates. Lipopolysaccharide (LPS) of B. pseudomallei is a potent immunostimulatory molecule comprised of lipid A, core, and O-polysaccharide (OPS) components. Four B. pseudomallei LPS types have been described based on SDS-PAGE patterns that represent the difference of OPS-type A, type B, type B2 and rough LPS. The majority of B. pseudomallei isolates are type A. We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) followed by electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QqTOF MS) and gas chromatography to characterize the lipid A of B. pseudomallei within LPS type A isolates. We determined that B. pseudomallei lipid A is represented by penta- and tetra-acylated species modified with 4-amino-4-deoxy-arabinose (Ara4N). The MALDI-TOF profiles from 171 clinical B. pseudomallei isolates, including 68 paired primary and relapse isolates and 35 within-host isolates were similar. We did not observe lipid A structural changes when the bacteria were cultured in different growth conditions. Dose-dependent NF-kappa B activation in HEK cells expressing TLR4 was observed using multiple heat-killed B. pseudomallei isolates and corresponding purified LPS. We demonstrated that TLR4-dependent NF-kappa B activation induced by heat-killed bacteria or LPS prepared from OPS deficient mutant was significantly greater than those induced by wild type B. pseudomallei. These findings suggest that the structure of B. pseudomallei lipid A is highly conserved in a wide variety of clinical and environmental circumstances but that the presence of OPS may modulate LPS-driven innate immune responses in melioidosis.
structure, lipid A, Burkholderia pseudomallei, immune response, melioidosis, TLR4
Structure type: oligomer ; 1948|
3. (CSDB ID: 12784) | report error |
| 3HOMyr-(1-3)-+ | ?%b-L-Arap4N-(1--P--1)--+ | | | Myr-(1-3)-3HOPam-(1-2)-+ | | | | | ?%b-L-Arap4N-(1--P--4)--b-D-GlcpN-(1-6)-a-D-GlcpN | | 3HOMyr-(1-3)-+ 3HOPam-(1-2)-+ | Show graphically |
|
Show legend Show as text |
Burkholderia pseudomallei K96243
(NCBI TaxID 272560,
species name lookup)
, ICD11: XN3LD ]; infection due to Burkholderia pseudomallei [ICD11: XN3LD ]tropmedres.acBurkholderia pseudomallei is an environmental bacterium that causes melioidosis, a major community-acquired infection in tropical regions. Melioidosis presents with a range of clinical symptoms, is often characterized by a robust inflammatory response, may relapse after treatment, and results in high mortality rates. Lipopolysaccharide (LPS) of B. pseudomallei is a potent immunostimulatory molecule comprised of lipid A, core, and O-polysaccharide (OPS) components. Four B. pseudomallei LPS types have been described based on SDS-PAGE patterns that represent the difference of OPS-type A, type B, type B2 and rough LPS. The majority of B. pseudomallei isolates are type A. We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) followed by electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QqTOF MS) and gas chromatography to characterize the lipid A of B. pseudomallei within LPS type A isolates. We determined that B. pseudomallei lipid A is represented by penta- and tetra-acylated species modified with 4-amino-4-deoxy-arabinose (Ara4N). The MALDI-TOF profiles from 171 clinical B. pseudomallei isolates, including 68 paired primary and relapse isolates and 35 within-host isolates were similar. We did not observe lipid A structural changes when the bacteria were cultured in different growth conditions. Dose-dependent NF-kappa B activation in HEK cells expressing TLR4 was observed using multiple heat-killed B. pseudomallei isolates and corresponding purified LPS. We demonstrated that TLR4-dependent NF-kappa B activation induced by heat-killed bacteria or LPS prepared from OPS deficient mutant was significantly greater than those induced by wild type B. pseudomallei. These findings suggest that the structure of B. pseudomallei lipid A is highly conserved in a wide variety of clinical and environmental circumstances but that the presence of OPS may modulate LPS-driven innate immune responses in melioidosis.
structure, lipid A, Burkholderia pseudomallei, immune response, melioidosis, TLR4
Structure type: oligomer ; 1932|
4. (CSDB ID: 12976) | report error |
| 3HOMyr-(1-3)-+ | b-L-Arap4N-(1--P--1)--+ | | | Myr-(1-3)-+ | | | | | 3HOPam-(1-2)-b-D-GlcpN-(1-6)-a-D-GlcpN | | P-4)-+ 3HOPam-(1-2)-+ | Show graphically |
|
Show legend Show as text |
Burkholderia pseudomallei K96243
(NCBI TaxID 272560,
species name lookup)
, ICD11: XN3LD ]; infection due to Burkholderia pseudomallei [ICD11: XN3LD ]tropmedres.acBurkholderia pseudomallei is an environmental bacterium that causes melioidosis, a major community-acquired infection in tropical regions. Melioidosis presents with a range of clinical symptoms, is often characterized by a robust inflammatory response, may relapse after treatment, and results in high mortality rates. Lipopolysaccharide (LPS) of B. pseudomallei is a potent immunostimulatory molecule comprised of lipid A, core, and O-polysaccharide (OPS) components. Four B. pseudomallei LPS types have been described based on SDS-PAGE patterns that represent the difference of OPS-type A, type B, type B2 and rough LPS. The majority of B. pseudomallei isolates are type A. We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) followed by electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QqTOF MS) and gas chromatography to characterize the lipid A of B. pseudomallei within LPS type A isolates. We determined that B. pseudomallei lipid A is represented by penta- and tetra-acylated species modified with 4-amino-4-deoxy-arabinose (Ara4N). The MALDI-TOF profiles from 171 clinical B. pseudomallei isolates, including 68 paired primary and relapse isolates and 35 within-host isolates were similar. We did not observe lipid A structural changes when the bacteria were cultured in different growth conditions. Dose-dependent NF-kappa B activation in HEK cells expressing TLR4 was observed using multiple heat-killed B. pseudomallei isolates and corresponding purified LPS. We demonstrated that TLR4-dependent NF-kappa B activation induced by heat-killed bacteria or LPS prepared from OPS deficient mutant was significantly greater than those induced by wild type B. pseudomallei. These findings suggest that the structure of B. pseudomallei lipid A is highly conserved in a wide variety of clinical and environmental circumstances but that the presence of OPS may modulate LPS-driven innate immune responses in melioidosis.
structure, lipid A, Burkholderia pseudomallei, immune response, melioidosis, TLR4
Structure type: oligomer ; 1575| New query | Export IDs | Home | Help |
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