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1. (Article ID: 12795)
Vilegas W, Vilegas JHY, Dachtler M, Glaser T, Albert K
Application of on-line C30 RP-HPLC-NMR for the analysis of flavonoids from leaf extract of Maytenus aquifolium
Phytochemical Analysis 11(5) (2000)
317-321
The application of on-line C30-reversed-phase high-pressure liquid chromatography–nuclear magnetic resonance spectroscopy is described for the analysis of tetraglycosylated flavonoids in aqueous and hydroalcoholic extracts of the leaves of Maytenus aquifolium (Celastraceae). Triacontyl stationary phases showed adequate separation for on-line 1H-NMR measurements at 600 MHz and allowed the characterisation of these flavonoids by detection of both aromatic and anomeric proton signals.
on-line HPLC-NMR; C30-stationary phase; flavonoids; Maytenus (Celastraceae)
Publication DOI: 10.1002/1099-1565(200009/10)11:5<317::AID-PCA529>3.0.CO;2-KJournal NLM ID: 9200492Publisher: Chichester, Sussex, UK: Wiley
Correspondence: vilegasw
iq.unesp.br
Institutions: Universidade Estadual Paulista, Instituto de Química de Araraquara, Araraquara, Brazil, Universidade de São Paulo, Instituto de Química de São Carlos, São Carlos, Brazil, Universität Tübingen, Institut für Organische Chemie, Tübingen, Germany
Methods: 1H NMR, HPLC, extraction
The publication contains the following compound(s):
- Compound ID: 33147
|
a-L-Rhap-(1-6)-+
|
b-D-Glcp-(1-3)-a-L-Rhap-(1-2)-b-D-Galp-(1-3)-Quercetin |
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Structure type: oligomer
Compound class: glycoside, flavonoid glycoside
- Compound ID: 33148
|
a-L-Rhap-(1-6)-+
|
b-D-Glcp-(1-3)-a-L-Rhap-(1-2)-b-D-Galp-(1-3)-Kaempferol |
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Structure type: oligomer
Compound class: glycoside, flavonoid glycoside
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2. (Article ID: 12796)
Vincken J-P, Borkhardt B, Bush M, Doeswijk-Voragen C, Dopico B, Labrador E, Lange L, McCann M, Morvan C, Muñoz F, Oomen R, Peugnet I, Rudolph B, Schols H, Sørensen S, Ulvskov P, Voragen A, Visser R
Remodelling pectin structure in potato
Developments in Plant Genetics and Breeding 6 (2000)
245-256
Pectin is a collection of polysaccharides, which play an important role in controlling the pore size of the plant cell wall, regulating cell-cell adhesion, and providing a source of sig-nalling molecules that elicit a range of cellular responses. Apart from this, pectins are of interest because they are an attractive hydrocolloid for various food applications. The kind and distribution of decorative groups in the pectic molecules largely determines for which application a particular pectin is most suitable. After the extraction of starch from potato tubers, a by-product is obtained, which is relatively rich in pectin. However, the quality of these pectins is poor compared to that from other sources such as citrus and apple. Rather than trying to change the structural characteristics of potato pectin post-harvest, we have embarked on achieving this in the potato plant itself. This paper summarises the structural features of pectin, the distribution of various pectic epitopes in tuber cell walls, the enzymes involved in its biosynthesis and degradation, and strategies employed to alter its fine structure in planta.
structure, potato, pectin
Publication DOI: 10.1016/S0168-7972(00)80129-2Publisher: Netherlands: Elsevier
Institutions: Wageningen University, Laboratory of Plant Breeding, Wageningen, The Netherlands, Biotechnology Group, DIAS, Frederiksberg, Denmark, Wageningen University, Department of Food Science, Wageningen, The Netherlands, University of Rouen, SCUEOR ESA 6037 CNRS 76821, Mont Saint Aignan, France, Parois et Polymeres Pariétaux, SCUEOR ESA 6037 CNRS 76821, Mont Saint Aignan, France, Universidad de Salamanca, Dept, de Biologia Vegetal (Fisiologia Vegetal), Salamanca, Spain, John Innes Centre, Department of Cell Biology, Colney, UK, Novo Nordisk A/S, Application Technology, Enzyme Development and Applications, Bagsvaerd, Denmark, Hercules, Copenhagen Pectins, Lille Skensved, Denmark
The publication contains the following compound(s):
- Compound ID: 33149
|
/Variants 0/-+
|
-4)-a-D-Galp-(1-
/Variants 0/ is:
?%Me-6)-
OR (inclusively)
?%Ac-3)-
OR (inclusively)
?%Ac-2)- |
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Structure type: structural motif or average structure
Compound class: polysaccharide
- Compound ID: 33150
Structure type: structural motif or average structure
Compound class: polysaccharide
- Compound ID: 33151
|
/Variants 0/-+
|
?%b-D-Galp-(1-4)-+ |
| |
-2)-a-L-Rhap-(1-4)-a-D-Galp-(1-
/Variants 0/ is:
?%Ac-3)-
OR (inclusively)
?%Ac-2)- |
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Structure type: structural motif or average structure
Compound class: polysaccharide
- Compound ID: 33152
|
/Variants 0/-+
|
-5)-a-L-Araf-(1-
/Variants 0/ is:
?%a-L-Araf-(1-3)-?%a-L-Araf-(1-3)-
OR (inclusively)
?%a-L-Araf-(1-2)- |
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Structure type: structural motif or average structure
Compound class: polysaccharide
- Compound ID: 33153
Structure type: structural motif or average structure
Compound class: polysaccharide
- Compound ID: 33154
|
a-L-Araf-(1-3)-+
|
a-L-Araf-(1-3)-+ |
| |
a-L-Araf-(1-6)-?%b-D-Galp-(1-6)-?%b-D-Galp-(1-6)-?%b-D-Galp-(1-6)-+
|
-3)-b-D-Galp-(1- |
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Structure type: structural motif or average structure
Compound class: polysaccharide
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