Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 29357266Publication DOI: 10.1139/cjm-2017-0620Journal NLM ID: 0372707Publisher: National Research Council of Canada
Correspondence: Boyang Cao <boyangcao
nankai.edu.cn>; Lei Wang <wanglei
nankai.edu.cn>
Institutions: Nankai University, 12538, TEDA Institute of Biological Sciences and Biotechnology, Tianjin, China
Capsular polysaccharides (CPSs), or K-antigens, are the major surface antigens of Escherichia coli. More than 80 serologically unique K-antigens are classified into four groups (Groups 1-4) of capsules. Group 1 and 4 contain the Wzy-dependent polymerization pathway and the gene clusters are in the order galF to gnd; Group 2 and 3 contain the ABC transporter-dependent pathway and the gene clusters consist of three regions, region 1, 2 and 3. Little is known about the variations among the gene clusters. In this study, nine serotypes of K-antigen gene clusters (K2ab, K11, K20, K24, K38, K84, K92, K96, and K102), were sequenced and correlated with their CPS chemical structures. Based on the sequence data, a K-antigen specific suspension array that detects 10 distinct CPSs, including the above nine CPSs plus K30, was developed. This is the first report to catalog the genetic features of E. coli K-antigen variations, and to develop a suspension array for their molecular typing. The method has a number of advantages over traditional bacteriophage and serum agglutination methods, and lays the foundation for straightforward identification and detection of additional K-antigens in the future.
antigen, K-antigen, identification, gene cluster, biotechnology, E.coli, suspension array, biological sciences
Structure type: polymer chemical repeating unit
Location inside paper: fig.2a,K38
Compound class: O-polysaccharide, K-antigen, O-antigen, CPS
Contained glycoepitopes: IEDB_130648,IEDB_134627,IEDB_136044,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_141807,IEDB_147450,IEDB_149136,IEDB_151531,IEDB_190606,SB_165,SB_166,SB_187,SB_195,SB_23,SB_24,SB_7,SB_8,SB_88
Methods: PCR, DNA sequencing, genetic methods, function analysis of gene clusters
Biosynthesis and genetic data: K-antigen gene clusters in E. coli
Related record ID(s): 12833, 12834, 12835, 12837, 12838, 12839, 12840, 13004
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G31519FO, GlycomeDB:
27606
Show glycosyltransferases
There is only one chemically distinct structure:
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