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Taxonomic group: plant / Streptophyta (Phylum: Streptophyta)

NCBI PubMed ID: 1422135
Publication DOI: 10.1007/BF00731160
Journal NLM ID: 8603310
Publisher: Kluwer Academic Publishers
Institutions: Institute de Biotechnologie, Université de Limoges, Limoges, France

Peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidase F (PNGase F) and endo-β-N-acetyl glucosaminidase F (Endo F) activities were monitored during cultivation of Flavobacterium meningosepticum using a new fluorescence-HPLC procedure based on a commercially available substrate. The PNGase F activity reached a maximum level at the end of the log phase and remained constant during the stationary phase, while Endo F continuously increased until late stationary phase. PNGase F obtained at the end of the log phase was less contaminated by other proteins compared with late stationary phase.

endoglycosidases assay, PNGase F, fluorescence-HPLC

Structure type: oligomer
Location inside paper: figure 1
Aglycon: resorufin-labeled peptide
Compound class: N-glycan
Contained glycoepitopes: IEDB_130701,IEDB_135813,IEDB_137340,IEDB_137485,IEDB_140116,IEDB_141793,IEDB_141807,IEDB_141828,IEDB_144983,IEDB_149158,IEDB_151079,IEDB_151531,IEDB_152206,IEDB_153220,IEDB_164174,IEDB_429156,IEDB_857734,IEDB_983930,SB_197,SB_198,SB_44,SB_67,SB_72,SB_73,SB_77

Methods: enzymatic digestion, fluorescence-HPLC

NCBI Taxonomy refs (TaxIDs): 3847
Reference(s) to other database(s): CCSD:30787, CBank-STR:18404
Show glycosyltransferases

There is only one chemically distinct structure:

CC(=O)N[C@H]1[C@H](O[C@@H]2[C@@H](CO)O[C@@H](NC(=O)CC(N)C(=O)O)[C@H](NC(C)=O)[C@H]2O)O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO[C@H]3O[C@H](CO[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H](O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H]3O)[C@@H](O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]3O)[C@@H]2O)[C@@H]1O

SVG MW SMILES 3D mol Ionize