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Zabłotni A, Matusiak D, Arbatsky NP, Moryl M, Maciejewska A, Kondakova AN, Shashkov AS, Ługowski C, Knirel YA, Rozalski A
Changes in the lipopolysacharide of Proteus mirabilis 9Bm (O11a)clinical strain in response to planktonic or biofilm type of growth
Medical Microbiology and Immunology 207(2) (2018)
129-139
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a-D-Glcp-(1-6)-+
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b-D-GlcpNAc-(1-2)-+ L-Thr-(2-6)-+ |
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-4)-b-D-GlcpA-(1-3)-b-D-GalpA-(1-3)-b-D-GlcpNAc-(1- |
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Proteus mirabilis O11a,b 24/57
(Ancestor NCBI TaxID 584,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Proteus mirabilis [ICD11:
XN9ZF 
]
NCBI PubMed ID: 29330591Publication DOI: 10.1007/s00430-018-0534-5Journal NLM ID: 0314524Publisher: Springer
Correspondence: agnieszka.zablotni
biol.uni.lodz.pl
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Laboratory of General Microbiology, Department of Biology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland, Department of Biology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland, Department of Immunochemistry, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolf Weigl 12, 53-114, Wroclaw, Poland
The impact of planktonic and biofilm lifestyles of the clinical isolate Proteus mirabilis 9B-m on its lipopolysaccharide (O-polysaccharide, core region, and lipid A) was evaluated. Proteus mirabilis bacteria are able to form biofilm and lipopolysaccharide is one of the factors involved in the biofilm formation. Lipopolysaccharide was isolated from planktonic and biofilm cells of the investigated strain and analyzed by SDS-PAGE with silver staining, Western blotting and ELISA, as well as NMR and matrix-assisted laser desorption ionization time-of-flight mass spectrometry techniques. Chemical and NMR spectroscopic analyses revealed that the structure of the O-polysaccharide of P. mirabilis 9B-m strain did not depend on the form of cell growth, but the full-length chains of the O-antigen were reduced when bacteria grew in biofilm. The study also revealed structural modifications of the core region in the lipopolysaccharide of biofilm-associated cells-peaks assigned to compounds absent in cells from the planktonic culture and not previously detected in any of the known Proteus core oligosaccharides. No differences in the lipid A structure were observed. In summary, our study demonstrated for the first time that changes in the lifestyle of P. mirabilis bacteria leads to the modifications of their important virulence factor-lipopolysaccharide.
Lipopolysaccharide, microbiology, immunology, bacteria, Proteus, Proteus mirabilis, core region, biology, biotechnology, Biofilm, planktonic form, bacterium
Structure type: polymer biological repeating unit
Location inside paper: fig.3, P. mirabilis 24/57 (O11a,b)
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115136,IEDB_135813,IEDB_137340,IEDB_140630,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_423153,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, SDS-PAGE, sugar analysis, ELISA, ESI-MS, GLC, Western blotting, MALDI-TOF MS, de-O-acetylation, GPC, acid degradation, analysis of TNF‑α secretion
Biological activity: serological data
Comments, role: Proteus mirabilis serogroup O11
Related record ID(s): 13006
NCBI Taxonomy refs (TaxIDs): 584
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Zabłotni A, Matusiak D, Arbatsky NP, Moryl M, Maciejewska A, Kondakova AN, Shashkov AS, Ługowski C, Knirel YA, Rozalski A
Changes in the lipopolysacharide of Proteus mirabilis 9Bm (O11a)clinical strain in response to planktonic or biofilm type of growth
Medical Microbiology and Immunology 207(2) (2018)
129-139
|
b-D-GlcpNAc-(1-2)-+ L-Thr-(2-6)-+
| |
-4)-b-D-GlcpA-(1-3)-b-D-GalpA-(1-3)-b-D-GlcpNAc-(1- |
Show graphically |
Proteus mirabilis O11a 9Bm
(Ancestor NCBI TaxID 584,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Proteus mirabilis [ICD11:
XN9ZF ]
The structure was elucidated in this paperNCBI PubMed ID: 29330591Publication DOI: 10.1007/s00430-018-0534-5Journal NLM ID: 0314524Publisher: Springer
Correspondence: agnieszka.zablotni
biol.uni.lodz.pl
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Laboratory of General Microbiology, Department of Biology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland, Department of Biology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland, Department of Immunochemistry, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolf Weigl 12, 53-114, Wroclaw, Poland
The impact of planktonic and biofilm lifestyles of the clinical isolate Proteus mirabilis 9B-m on its lipopolysaccharide (O-polysaccharide, core region, and lipid A) was evaluated. Proteus mirabilis bacteria are able to form biofilm and lipopolysaccharide is one of the factors involved in the biofilm formation. Lipopolysaccharide was isolated from planktonic and biofilm cells of the investigated strain and analyzed by SDS-PAGE with silver staining, Western blotting and ELISA, as well as NMR and matrix-assisted laser desorption ionization time-of-flight mass spectrometry techniques. Chemical and NMR spectroscopic analyses revealed that the structure of the O-polysaccharide of P. mirabilis 9B-m strain did not depend on the form of cell growth, but the full-length chains of the O-antigen were reduced when bacteria grew in biofilm. The study also revealed structural modifications of the core region in the lipopolysaccharide of biofilm-associated cells-peaks assigned to compounds absent in cells from the planktonic culture and not previously detected in any of the known Proteus core oligosaccharides. No differences in the lipid A structure were observed. In summary, our study demonstrated for the first time that changes in the lifestyle of P. mirabilis bacteria leads to the modifications of their important virulence factor-lipopolysaccharide.
Lipopolysaccharide, microbiology, immunology, bacteria, Proteus, Proteus mirabilis, core region, biology, biotechnology, Biofilm, planktonic form, bacterium
Structure type: polymer biological repeating unit
Location inside paper: fig.3, P. mirabilis 9Bm (O11a)
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115136,IEDB_135813,IEDB_137340,IEDB_140630,IEDB_141807,IEDB_151531,IEDB_423153
Methods: 13C NMR, 1H NMR, SDS-PAGE, sugar analysis, ELISA, ESI-MS, GLC, Western blotting, MALDI-TOF MS, de-O-acetylation, GPC, acid degradation, analysis of TNF‑α secretion
Biological activity: serological data
Comments, role: Proteus mirabilis serogroup O11a strain 9Bm
Related record ID(s): 12841
NCBI Taxonomy refs (TaxIDs): 584
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