The structure was elucidated in this paper NCBI PubMed ID:29929050 Publication DOI:10.1016/j.carres.2018.06.006 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: zdorovenkoemail.ru Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, 220141, Minsk, Belarus, Institute of Microbiology, National Academy of Sciences of Belarus, 220141, Minsk, Belarus
Strain of Pseudomonas psychrotolerans was cultured on the nutrient agar and in a liquid nutrient broth. Bacterial cells were phage-typed with bacteriophages specific to Pseudomonas. O-antigen was isolated from cells using phenol-water method and mild acid degradation. The following structures of the polysaccharides extracted were established by sugar analysis and 1D, 2D NMR spectroscopy: PSI→3)-α-D-Manp-(1→2)-α-D-Manp-(1→; PSII→3)-α-D-Rhap-(1→2)-β-D-Rhap-(1→3)-α-D-Rhap-(1→; α-D-Glcp-(1˩; 2.
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, acid hydrolysis, GLC, GPC, mild acid degradation, phage typing Comments, role: it is not clear what residue of rhamnose is attached to the side residue of aDGlcp(1-2); the ralative ratio of PSI:PSII was ~ 1:1.
Related record ID(s): 13010 NCBI Taxonomy refs (TaxIDs):237610 Reference(s) to other database(s): GTC:G73863YN Show glycosyltransferases
The structure was elucidated in this paper NCBI PubMed ID:29929050 Publication DOI:10.1016/j.carres.2018.06.006 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: zdorovenkoemail.ru Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, 220141, Minsk, Belarus, Institute of Microbiology, National Academy of Sciences of Belarus, 220141, Minsk, Belarus
Strain of Pseudomonas psychrotolerans was cultured on the nutrient agar and in a liquid nutrient broth. Bacterial cells were phage-typed with bacteriophages specific to Pseudomonas. O-antigen was isolated from cells using phenol-water method and mild acid degradation. The following structures of the polysaccharides extracted were established by sugar analysis and 1D, 2D NMR spectroscopy: PSI→3)-α-D-Manp-(1→2)-α-D-Manp-(1→; PSII→3)-α-D-Rhap-(1→2)-β-D-Rhap-(1→3)-α-D-Rhap-(1→; α-D-Glcp-(1˩; 2.
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