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Bellich B, Lagatolla C, Tossi A, Benincasa M, Cescutti P, Rizzo R
Influence of Bacterial Biofilm Polysaccharide Structure on Interactions with Antimicrobial Peptides: A Study on Klebsiella pneumoniae
International Journal of Molecular Sciences 19(6) (2018)
Klebsiella pneumoniae KpTs101
(Ancestor NCBI TaxID 573,
species name lookup)
Klebsiella pneumoniae O1
(Ancestor NCBI TaxID 573,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: urinary tract infections (UTI) [ICD11:
GC08 
];
infection due to Klebsiella pneumoniae [ICD11:
XN741 ]
NCBI PubMed ID: 29882774Publication DOI: 10.3390/ijms19061685Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: R. Rizzo <izzor
units.it>
Institutions: Department of Life Sciences, University of Trieste, Via Licio Giorgieri 1, 34127 Trieste, Italy
Biofilms are complex systems produced by bacteria and constituted by macromolecular matrix embedding cells. They provide advantages to bacteria including protection against antimicrobials. The protection given by biofilms produced by Klebsiella pneumoniae strains towards antimicrobial peptides of the innate immune system was investigated. In particular, the role of matrix bacterial exopolysaccharides was explored. Three clinical strains producing exopolysaccharides with different chemistry were selected and the interaction of purified biofilm polysaccharides with two bovine cathelicidins was studied by circular dichroism spectroscopy and microbiological assays to establish their influence on the peptide’s antimicrobial activity. The spectroscopic data indicated a different extent of interaction with the two peptides, in a manner dependent on their sugar composition, and in particular the presence of rhamnose residues correlated with a lower interaction. The extent of interaction was then related to the protection towards antimicrobial peptides, conferred by the addition of the different exopolysaccharides, in minimum inhibitory concentration (MIC) assays against a reference Escherichia coli strain. Microbiological results were in very good agreement with spectroscopic data, confirming the active role of matrix polysaccharides in determining a biofilm’s protective capacity and indicating lower protection levels afforded by rhamnose containing exopolysaccharides.
Escherichia coli, antimicrobial peptides, bacterial biofilm, bacterial protection, exopolysaccharides biological activity, Cathelicidins, matrix exopolysaccharides
Structure type: polymer chemical repeating unit
Location inside paper: table 1, KpTs101
Trivial name: D-galactan I, galactan I, galactan, D-galactan, microbial carbohydrate determinants, D-galactan-I
Compound class: CPS, EPS, O-polysaccharide, O-antigen, LPS, cell wall polysaccharide
Contained glycoepitopes: IEDB_136095,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_190606,SB_7
Methods: CD, antimicrobial peptide sensitivity assays
Biological activity: EPS from Klebsiella isolates were more resistant (twice) than E. coli ML-35 to bovine cathelicidins: BMAP-27 and Bac7
Related record ID(s): 13029, 13030, 13031
NCBI Taxonomy refs (TaxIDs): 573Reference(s) to other database(s): GTC:G85324FU, GlycomeDB:
665
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There is only one chemically distinct structure:
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Bellich B, Lagatolla C, Tossi A, Benincasa M, Cescutti P, Rizzo R
Influence of Bacterial Biofilm Polysaccharide Structure on Interactions with Antimicrobial Peptides: A Study on Klebsiella pneumoniae
International Journal of Molecular Sciences 19(6) (2018)
Klebsiella pneumoniae KpTs101
(Ancestor NCBI TaxID 573,
species name lookup)
Klebsiella pneumoniae O1
(Ancestor NCBI TaxID 573,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: urinary tract infections (UTI) [ICD11:
GC08 ];
infection due to Klebsiella pneumoniae [ICD11:
XN741 ]
NCBI PubMed ID: 29882774Publication DOI: 10.3390/ijms19061685Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: R. Rizzo <izzor
units.it>
Institutions: Department of Life Sciences, University of Trieste, Via Licio Giorgieri 1, 34127 Trieste, Italy
Biofilms are complex systems produced by bacteria and constituted by macromolecular matrix embedding cells. They provide advantages to bacteria including protection against antimicrobials. The protection given by biofilms produced by Klebsiella pneumoniae strains towards antimicrobial peptides of the innate immune system was investigated. In particular, the role of matrix bacterial exopolysaccharides was explored. Three clinical strains producing exopolysaccharides with different chemistry were selected and the interaction of purified biofilm polysaccharides with two bovine cathelicidins was studied by circular dichroism spectroscopy and microbiological assays to establish their influence on the peptide’s antimicrobial activity. The spectroscopic data indicated a different extent of interaction with the two peptides, in a manner dependent on their sugar composition, and in particular the presence of rhamnose residues correlated with a lower interaction. The extent of interaction was then related to the protection towards antimicrobial peptides, conferred by the addition of the different exopolysaccharides, in minimum inhibitory concentration (MIC) assays against a reference Escherichia coli strain. Microbiological results were in very good agreement with spectroscopic data, confirming the active role of matrix polysaccharides in determining a biofilm’s protective capacity and indicating lower protection levels afforded by rhamnose containing exopolysaccharides.
Escherichia coli, antimicrobial peptides, bacterial biofilm, bacterial protection, exopolysaccharides biological activity, Cathelicidins, matrix exopolysaccharides
Structure type: polymer chemical repeating unit
Location inside paper: table 1, KpTs101
Trivial name: D-galactan II, galactan II, D-galactan-II
Compound class: CPS, EPS, O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_149558,IEDB_151528,IEDB_190606,IEDB_918314,SB_165,SB_166,SB_187,SB_195,SB_7,SB_87,SB_88
Methods: CD, antimicrobial peptide sensitivity assays
Biological activity: EPS from Klebsiella isolates were more resistant (twice) than E. coli ML-35 to bovine cathelicidins: BMAP-27 and Bac7
Related record ID(s): 12869, 13030, 13031
NCBI Taxonomy refs (TaxIDs): 573Reference(s) to other database(s): GTC:G16623PZ, GlycomeDB:
664
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There is only one chemically distinct structure:
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Bellich B, Lagatolla C, Tossi A, Benincasa M, Cescutti P, Rizzo R
Influence of Bacterial Biofilm Polysaccharide Structure on Interactions with Antimicrobial Peptides: A Study on Klebsiella pneumoniae
International Journal of Molecular Sciences 19(6) (2018)
|
b-D-Manp-(1-4)-+
|
-2)-a-D-GlcpA-(1-3)-a-D-Manp-(1-2)-a-D-Manp-(1-3)-b-D-Glcp-(1- |
Show graphically |
Klebsiella pneumoniae KpTs113
(Ancestor NCBI TaxID 573,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: urinary tract infections (UTI) [ICD11:
GC08 ];
infection due to Klebsiella pneumoniae [ICD11:
XN741 ]
NCBI PubMed ID: 29882774Publication DOI: 10.3390/ijms19061685Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: R. Rizzo <izzor
units.it>
Institutions: Department of Life Sciences, University of Trieste, Via Licio Giorgieri 1, 34127 Trieste, Italy
Biofilms are complex systems produced by bacteria and constituted by macromolecular matrix embedding cells. They provide advantages to bacteria including protection against antimicrobials. The protection given by biofilms produced by Klebsiella pneumoniae strains towards antimicrobial peptides of the innate immune system was investigated. In particular, the role of matrix bacterial exopolysaccharides was explored. Three clinical strains producing exopolysaccharides with different chemistry were selected and the interaction of purified biofilm polysaccharides with two bovine cathelicidins was studied by circular dichroism spectroscopy and microbiological assays to establish their influence on the peptide’s antimicrobial activity. The spectroscopic data indicated a different extent of interaction with the two peptides, in a manner dependent on their sugar composition, and in particular the presence of rhamnose residues correlated with a lower interaction. The extent of interaction was then related to the protection towards antimicrobial peptides, conferred by the addition of the different exopolysaccharides, in minimum inhibitory concentration (MIC) assays against a reference Escherichia coli strain. Microbiological results were in very good agreement with spectroscopic data, confirming the active role of matrix polysaccharides in determining a biofilm’s protective capacity and indicating lower protection levels afforded by rhamnose containing exopolysaccharides.
Escherichia coli, antimicrobial peptides, bacterial biofilm, bacterial protection, exopolysaccharides biological activity, Cathelicidins, matrix exopolysaccharides
Structure type: polymer chemical repeating unit
Location inside paper: table 1, KpTs113
Compound class: CPS, EPS
Contained glycoepitopes: IEDB_115136,IEDB_130701,IEDB_136104,IEDB_137485,IEDB_140630,IEDB_142488,IEDB_143632,IEDB_144983,IEDB_146664,IEDB_152206,IEDB_983930,IEDB_983931,SB_136,SB_192,SB_196,SB_44,SB_67,SB_72
Methods: CD, antimicrobial peptide sensitivity assays
Biological activity: EPS from Klebsiella isolates were more resistant (twice) than E. coli ML-35 to bovine cathelicidins: BMAP-27 and Bac7
Related record ID(s): 12869, 13029, 13031
NCBI Taxonomy refs (TaxIDs): 573Reference(s) to other database(s): GTC:G51668JD, GlycomeDB:
37063
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There is only one chemically distinct structure:
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Bellich B, Lagatolla C, Tossi A, Benincasa M, Cescutti P, Rizzo R
Influence of Bacterial Biofilm Polysaccharide Structure on Interactions with Antimicrobial Peptides: A Study on Klebsiella pneumoniae
International Journal of Molecular Sciences 19(6) (2018)
|
a-L-Rhap-(1-4)-+
|
-2)-a-L-Rhap-(1-2)-a-L-Rhap-(1-2)-a-D-Glcp-(1-3)-b-D-Galp-(1-3)-a-D-GalpA-(1- |
Show graphically |
Klebsiella pneumoniae KpMn7
(Ancestor NCBI TaxID 573,
species name lookup)
Klebsiella pneumoniae 2796
(Ancestor NCBI TaxID 573,
species name lookup)
Klebsiella pneumoniae 3264
(Ancestor NCBI TaxID 573,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: urinary tract infections (UTI) [ICD11:
GC08 ];
infection due to Klebsiella pneumoniae [ICD11:
XN741 ]
NCBI PubMed ID: 29882774Publication DOI: 10.3390/ijms19061685Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: R. Rizzo <izzor
units.it>
Institutions: Department of Life Sciences, University of Trieste, Via Licio Giorgieri 1, 34127 Trieste, Italy
Biofilms are complex systems produced by bacteria and constituted by macromolecular matrix embedding cells. They provide advantages to bacteria including protection against antimicrobials. The protection given by biofilms produced by Klebsiella pneumoniae strains towards antimicrobial peptides of the innate immune system was investigated. In particular, the role of matrix bacterial exopolysaccharides was explored. Three clinical strains producing exopolysaccharides with different chemistry were selected and the interaction of purified biofilm polysaccharides with two bovine cathelicidins was studied by circular dichroism spectroscopy and microbiological assays to establish their influence on the peptide’s antimicrobial activity. The spectroscopic data indicated a different extent of interaction with the two peptides, in a manner dependent on their sugar composition, and in particular the presence of rhamnose residues correlated with a lower interaction. The extent of interaction was then related to the protection towards antimicrobial peptides, conferred by the addition of the different exopolysaccharides, in minimum inhibitory concentration (MIC) assays against a reference Escherichia coli strain. Microbiological results were in very good agreement with spectroscopic data, confirming the active role of matrix polysaccharides in determining a biofilm’s protective capacity and indicating lower protection levels afforded by rhamnose containing exopolysaccharides.
Escherichia coli, antimicrobial peptides, bacterial biofilm, bacterial protection, exopolysaccharides biological activity, Cathelicidins, matrix exopolysaccharides
Structure type: polymer chemical repeating unit
Location inside paper: table 1, KpMn7
Compound class: EPS
Contained glycoepitopes: IEDB_133754,IEDB_136044,IEDB_136105,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_190606,IEDB_225177,IEDB_885823,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
Methods: CD, antimicrobial peptide sensitivity assays
Biological activity: EPS from Klebsiella isolates were more resistant (twice) than E. coli ML-35 to bovine cathelicidins: BMAP-27 and Bac7
Related record ID(s): 12869, 13029, 13030
NCBI Taxonomy refs (TaxIDs): 573Reference(s) to other database(s): GTC:G14240MQ
Show glycosyltransferases
There is only one chemically distinct structure:
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