Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 30445812Publication DOI: 10.1021/acsinfecdis.8b00187Journal NLM ID: 101654580Publisher: Washington, DC: American Chemical Society
Correspondence: pwang11
gsu.edu
Institutions: Department of Chemistry, Georgia State University, 100 Piedmont Avenue, Atlanta, Georgia 30303, United States, National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, 27 ShanDa Nan Road, Jinan, Shandong 250100, China
Bacterial pathogen infections are fast-growing public health threats and worldwide problems. Glycoconjugate vaccines are among the most effective means in combating such infections. Recent advances in bacterial protein glycan coupling technology (PGCT) have revolutionized the production of glycoconjugate vaccines and drawn enormous attention from both researchers and pharmaceutical companies. Cloning of bacterial surface polysaccharide gene cluster is a prerequisite for the application of PGCT. In this study, we applied the RecET direct cloning strategy for rapid and efficient cloning of O-antigen polysaccharide gene clusters from Escherichia coli serotypes O25b, O26, and O55 in a high-fidelity manner. Then, these gene clusters were applied in PGCT to produce corresponding glycoconjugates. Subsequent immunological studies verified the abilities of glycoconjugate vaccine candidates O25-maltose-binding protein (MBP), O26-MBP, and O55-MBP to generate serotype-specific antibodies and confer protection against E. coli infections. The combination of RecET direct cloning and PGCT makes the rapid production of glycoconjugate vaccines against fast-expanding bacterial pathogens possible.
glycoengineering, glycoconjugate vaccine, bacterial surface polysaccharide gene cluster, PGCT, RecET direct cloning
Structure type: polymer chemical repeating unit
Location inside paper: fig.1A, E.coli O25b
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135813,IEDB_136105,IEDB_137340,IEDB_141806,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_225177,IEDB_885823,IEDB_983931,SB_192
Methods: PCR, SDS-PAGE, DNA techniques, Western blotting, serum bactericidal assays, immunological assays, conjugation, RecET direct cloning
Related record ID(s): 12675, 12676
NCBI Taxonomy refs (TaxIDs): 1095709Reference(s) to other database(s): GTC:G18223OD, GlycomeDB:
28106
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There is only one chemically distinct structure:
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