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Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_141807,IEDB_151528,IEDB_151531,IEDB_190606,SB_21,SB_7

• Article ID: 405
  Vinion-Dubiel AD, Goldberg JB "Lipopolysaccharide of Burkholderia cepacia complex" - Journal of Endotoxin Research 9(4) (2003) 201-213
  

  Burkholderia cepacia complex (Bcc) is a group of phenotypically similar, genetically distinct bacteria that are beneficial to the environment but can also cause severe human infections. Bcc are being exploited for use as bioremediation agents and as a way to combat agricultural plant diseases. However, Bcc can cause lung infections in patients with chronic granulomatous disease or cystic fibrosis often resulting in mortality of these patients. Since it is unclear what bacterial components are necessary for causing human infections, studies of Bcc have focused on identifying putative virulence factors. As in other Gram-negative bacteria, the lipopolysaccharide (LPS) of Bcc induces a strong immune response that can contribute to host cell damage. The unusual structure of Bcc LPS lowers the anionic charge of the Bcc cell surface, which inhibits the binding and subsequent effects of cationic antibiotics. These distinguishing features include the substitution of a Ko for a Kdo residue in the inner core oligosaccharide and Ara4N residues bound to phosphates of the lipid A backbone. The structures of O antigen subunits and the consequent serotypes will also be discussed, with particular reference to the O antigen biosynthetic loci of two Bcc strains.

  Lipopolysaccharide, structure, Burkholderia, Burkholderia cepacia, O-antigen, complex, endotoxin, Re

  NCBI PubMed ID: 12935351
  Publication DOI: 10.1177/09680519030090040101
  Journal NLM ID: 9433350
  Publisher: Maney Publishing
  Institutions: Department of Microbiology, University of Virginia Health Sciences Center, Charlottesville, VA 22908-0734, USA
  
   
  
  Burkholderia cepacia PVFi-5A
  CSDB ID 31609 (all data & tools)
• Article ID: 772
  Cimino P, Corsaro MM, De Castro C, Evidente A, Marciano CE, Parrilli M, Sfalanga A, Surico G "Structural determination of the O-deacetylated O-chain of lipopolysaccharide from Burkholderia_Pseudomonas cepacia strain PVFi-5A" - Carbohydrate Research 307 (1998) 333-341
  

  On the basis of chemical degradation methods and one-and two-dimensional 1H and 13C NMR experiments the novel following structure was established for the O-deacetylated repeating unit of the O-chain of the main Burkholderia_Pseudomonas cepacia (strain PVFi-5A) lipopolysaccharide: →4)-β-D-GalpNAc-(1→3)-α-D-Galp-(1→6)-α-D-GlcpNAc-(1→.

  Lipopolysaccharide, LPS, structure, strain, structural, polysaccharide, Burkholderia, Burkholderia cepacia, Pseudomonas, determination, structural determination, O-polysaccharide, O-specific, O-specific polysaccharide, Bacterial polysaccharide, O-chain, Pseudomonas cepacia

  NCBI PubMed ID: 9675371
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: parrilli@unina.it
  Institutions: Dipartimento di Chimica Organica e Biologica, Universita di Napoli Federico II, via Mezzocannone 16,I-80134 Napoli, Italy, Dipartimento di Scienze Chimico-Agrarie, Universita di Napoli Federico II, via Universita 100, I-80055 Portici, Italy, Istituto di Patologia e Zoologia Forestale e Agraria, Universita di Firenze, Piazzale delle Cascine 28, 50144 Firenze, Italy
  Methods: methylation, NMR-2D, NMR, de-O-acylation, sugar analysis, acid hydrolysis, Smith degradation
  
   
  
  Burkholderia cepacia PVFi-5A
  CSDB ID 1232 (all data & tools)
• Article ID: 4329
  Knirel YA "Structure of O-antigens" - Book: Bacterial lipopolysaccharides: Structure, chemical synthesis, biogenesis and interaction with host cells (2011) Chapter 3, 41-115
  

  The lipopolysaccharide (LPS) is the major constituent of the outer leaflet of the outer membrane of Gram-negative bacteria. Its lipid A moiety is embedded in the membrane and serves as an anchor for the rest of the LPS molecule. The outermost repetitive glycan region of the LPS is linked to the lipid A through a core oligosaccharide (OS), and is designated as the O-specific polysaccharide (O-polysaccharide, OPS) or O-antigen. The O-antigen is the most variable portion of the LPS and provides serological specificity, which is used for bacterial serotyping. The OPS also provides protection to the microorganisms from host defenses such as complement mediated killing and phagocytosis, and is involved in interactions of bacteria with plants and bacteriophages. Studies of the OPSs ranging from the elucidation of their chemical structures and conformations to their biological and physico-chemical properties help improving classification schemes of Gram-negative bacteria. Furthermore, these studies contributed to a better understanding of the mechanisms of pathogenesis of infectious diseases, as well as provided information to develop novel vaccines and diagnostic reagents.

  Lipopolysaccharide, synthesis, lipopolysaccharides, structure, Bacterial, host, O-antigen, O antigen, cell, O antigens, O-antigens, chemical, interaction, cells, PDF, chemical synthesis, biogenesis

  Publication DOI: 10.1007/978-3-7091-0733-1_3
  Publisher: Springer
  Correspondence: knirel@ioc.ac.ru
  Editors: Knirel YA, Valvano MA
  Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
  
   
  
  Burkholderia cepacia PVFi-5A
  CSDB ID 27879 (all data & tools)
• Article ID: 5143
  Cloutier M, Muru K, Ravicoularamin G, Gauthier C "Polysaccharides from Burkholderia species as targets for vaccine development, immunomodulation and chemical synthesis" - Natural Product Reports 35(12) (2018) 1251-1293
  

  Burkholderia species are a vast group of human pathogenic, phytopathogenic, and plant- or environment-associated bacteria. B. pseudomallei, B. mallei, and B. cepacia complex are the causative agents of melioidosis, glanders, and cystic fibrosis-related infections, respectively, which are fatal diseases in humans and animals. Due to their high resistance to antibiotics, high mortality rates, and increased infectivity via the respiratory tract, B. pseudomallei and B. mallei have been listed as potential bioterrorism agents by the Centers for Disease Control and Prevention. Burkholderia species are able to produce a large network of surface-exposed polysaccharides, i.e., lipopolysaccharides, capsular polysaccharides, and exopolysaccharides, which are virulence factors, immunomodulators, major biofilm components, and protective antigens, and have crucial implications in the pathogenicity of Burkholderia-associated diseases. This review provides a comprehensive and up-to-date account regarding the structural elucidation and biological activities of surface polysaccharides produced by Burkholderia species. The chemical synthesis of oligosaccharides mimicking Burkholderia polysaccharides is described in detail. Emphasis is placed on the recent research efforts toward the development of glycoconjugate vaccines against melioidosis and glanders based on synthetic or native Burkholderia oligo/polysaccharides.

  lipopolysaccharides, Burkholderia, capsular polysaccharides, Oligosaccharides, glycoconjugate vaccines, antigens, exopolysaccharides, surface polysaccharide, virulence factor, Biofilm, chemical synthesis, bioterrorism

  Publication DOI: 10.1039/C8NP00046H
  Journal NLM ID: 8502408
  Publisher: London: Royal Society of Chemistry
  Correspondence: charles.gauthier@iaf.inrs.ca
  Institutions: INRS-Institut Armand-Frappier, Universite du Quebec, 531, boul. des Prairies, Laval, Canada
  
   
  
  Burkholderia cepacia PVFi-5A
  CSDB ID 12504 (all data & tools)

Show legend Show as text

Structure type: oligomer
Contained glycoepitopes: IEDB_136906,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_151528,IEDB_190606,SB_7

• Article ID: 772
  Cimino P, Corsaro MM, De Castro C, Evidente A, Marciano CE, Parrilli M, Sfalanga A, Surico G "Structural determination of the O-deacetylated O-chain of lipopolysaccharide from Burkholderia_Pseudomonas cepacia strain PVFi-5A" - Carbohydrate Research 307 (1998) 333-341
  

  On the basis of chemical degradation methods and one-and two-dimensional 1H and 13C NMR experiments the novel following structure was established for the O-deacetylated repeating unit of the O-chain of the main Burkholderia_Pseudomonas cepacia (strain PVFi-5A) lipopolysaccharide: →4)-β-D-GalpNAc-(1→3)-α-D-Galp-(1→6)-α-D-GlcpNAc-(1→.

  Lipopolysaccharide, LPS, structure, strain, structural, polysaccharide, Burkholderia, Burkholderia cepacia, Pseudomonas, determination, structural determination, O-polysaccharide, O-specific, O-specific polysaccharide, Bacterial polysaccharide, O-chain, Pseudomonas cepacia

  NCBI PubMed ID: 9675371
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: parrilli@unina.it
  Institutions: Dipartimento di Chimica Organica e Biologica, Universita di Napoli Federico II, via Mezzocannone 16,I-80134 Napoli, Italy, Dipartimento di Scienze Chimico-Agrarie, Universita di Napoli Federico II, via Universita 100, I-80055 Portici, Italy, Istituto di Patologia e Zoologia Forestale e Agraria, Universita di Firenze, Piazzale delle Cascine 28, 50144 Firenze, Italy
  Methods: methylation, NMR-2D, NMR, de-O-acylation, sugar analysis, acid hydrolysis, Smith degradation
  
   
  
  Burkholderia cepacia PVFi-5A
  CSDB ID 1321 (all data & tools)