Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
NCBI PubMed ID: 8157600Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Institutions: Sección de Microbiología, Facultat de Farmacia, Universitat de Valencia, Spain
A method was devised for the isolation of yeast monomorphic (LEV) mutants of Candida albicans. By this procedure, about 20 stable yeast-like mutants were isolated after mutagenesis with ethyl methane sulfonate. The growth rate of the mutants in different carbon sources, both fermentable and not, was indistinguishable from that of the parental strain, but they were unable to grow as mycelial forms after application of any of the common effective inducers, i.e., heat shock, pH alterations, proline addition, or use of GlcNAc as the carbon source. Studies performed with one selected strain demonstrated that it had severe alterations in the chemical composition of the cell wall, mainly in the levels of chitin and glucans, and in specific mannoproteins, some of them recognizable by specific polyclonal and monoclonal antibodies. It is suggested that these structural alterations hinder the construction of a normal hyphal wall.
Structure type: homopolymer ; n is large
Trivial name: chitin
Compound class: chitin
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_423114,IEDB_423150,SB_74,SB_85
Methods: SDS-PAGE, ELISA, autoradiography, immunodetection
Biosynthesis and genetic data: genetic data
Related record ID(s): 42301, 42350, 49501, 49523, 49564, 49568, 50015, 50302, 50305, 50306, 50309, 50312, 50313, 50316, 50318, 111874, 121703, 139821, 139869, 143656, 147987, 149876
NCBI Taxonomy refs (TaxIDs): 5476Reference(s) to other database(s): GTC:G97099AY, CCSD:
50510, CBank-STR:4329
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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