O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide of nitrogen-fixing bacterium Azospirillum fermentarium CC-LY743(T) (IBPPM 578) and was studied by sugar analysis along with (1)H and (13)C NMR spectroscopy, including (1)H,(1)H COSY, TOCSY, ROESY, and (1)H,(13)C HSQC and HMBC experiments. The polysaccharide was found to be linear and to consist of alterating α-l-fucose and α-d-mannose residues in tetrasaccharide repeating units of the following structure: →2)-α-D-Manp-(1→3)-α-L-Fucp-(1→3)-α-D-Manp-(1→3)-α-L-Fucp -(1→.
Lipopolysaccharide, biochemistry, bacterial polysaccharide structure, Plants, physiology, Azospirillum fermentarium
NCBI PubMed ID: 29929051Publication DOI: 10.1016/j.carres.2018.06.003Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: si_elena@mail.ru
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov, 410049, Russia, N. G. Chernyshevsky Saratov State University, 83 Ulitsa Astrakhanskaya, Saratov, 410012, Russia
Methods: 13C NMR, 1H NMR, NMR-2D, SDS-PAGE, sugar analysis, acid hydrolysis, GLC, GPC
Found