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Hino K, Yamamoto S, Sano O, Taniguchi Y, Kohno K, Usui M, Fukuda S, Hanzawa H, Haruyama H, Kurimoto M
Carbohydrate structures of the glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen
Journal of Biochemistry 117 (1995)
289-295
|
b-D-GlcpNAc-(1-2)-a-D-Manp-(1-6)-+ a-L-Fucp-(1-3)-+
| |
b-D-GlcpNAc-(1-2)-a-D-Manp-(1-3)-b-D-Manp-(1-4)-D-GlcpNAc-(1-4)-D-GlcpNAc
|
b-D-Xylp-(1-2)-+ |
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Cryptomeria japonica
(NCBI TaxID 3369,
species name lookup)
Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: pollen
NCBI PubMed ID: 7608114Publication DOI: 10.1093/jb/117.2.289Journal NLM ID: 0376600Publisher: Japanese Biochemical Society
Institutions: Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., Okayama
The glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen was treated with pepsin and glycopeptidase A to release asparagine-linked oligosaccharides. The reducing ends of the oligosaccharides were aminated with the fluorescent reagent 2-aminopyridine. The oligosaccharide derivatives were purified by gel permeation chromatography and reversed-phase HPLC. Their structures were determined by sequential exoglycosidase digestion and 500 MHz 1H-NMR spectroscopy. Four oligosaccharide structures, A, B, C, and D, were identified as the xylose-containing complex-type. They were present at a molar ratio of 8:1:6:1. By amino acid sequence analyses of the tryptic peptides, Asn-170 and Asn-333 of Cry j I were found to carry asparagine-linked oligosaccharides.
glycoprotein, carbohydrate structure, 1H-NMR, allergen, Cry j I
Structure type: oligomer
Location inside paper: A
Compound class: N-glycan
Contained glycoepitopes: IEDB_114701,IEDB_115005,IEDB_116644,IEDB_122244,IEDB_123886,IEDB_123887,IEDB_123888,IEDB_130701,IEDB_135813,IEDB_136045,IEDB_137340,IEDB_137485,IEDB_141793,IEDB_141807,IEDB_142489,IEDB_144562,IEDB_144983,IEDB_145668,IEDB_145669,IEDB_146665,IEDB_148491,IEDB_148492,IEDB_148493,IEDB_150092,IEDB_151531,IEDB_152206,IEDB_152214,IEDB_153212,IEDB_167186,IEDB_167188,IEDB_167189,IEDB_174332,IEDB_174333,IEDB_548907,IEDB_983930,SB_197,SB_198,SB_33,SB_44,SB_67,SB_72,SB_73,SB_74,SB_85,SB_86
Methods: 1H NMR, HPLC, enzymatic digestion, CC, amino acid sequencing
Comments, role: Parent molecule: Cry j I; proposed structure
NCBI Taxonomy refs (TaxIDs): 3369Reference(s) to other database(s): GTC:G25906JM, CCSD:
47725, CBank-STR:18507
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There is only one chemically distinct structure:
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Hino K, Yamamoto S, Sano O, Taniguchi Y, Kohno K, Usui M, Fukuda S, Hanzawa H, Haruyama H, Kurimoto M
Carbohydrate structures of the glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen
Journal of Biochemistry 117 (1995)
289-295
|
b-D-GlcpNAc-(1-2)-a-D-Manp-(1-3)-+
|
a-L-Fucp-(1-6)-+ |
| |
b-D-Galp-(1-4)-b-D-GlcpNAc-(1-2)-a-D-Manp-(1-6)-b-D-Manp-(1-4)-b-D-GlcpNAc-(1-4)-D-GlcpNAc
|
b-D-Xylp-(1-2)-+ |
Show graphically |
Cryptomeria japonica
(NCBI TaxID 3369,
species name lookup)
Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: pollen
NCBI PubMed ID: 7608114Publication DOI: 10.1093/jb/117.2.289Journal NLM ID: 0376600Publisher: Japanese Biochemical Society
Institutions: Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., Okayama
The glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen was treated with pepsin and glycopeptidase A to release asparagine-linked oligosaccharides. The reducing ends of the oligosaccharides were aminated with the fluorescent reagent 2-aminopyridine. The oligosaccharide derivatives were purified by gel permeation chromatography and reversed-phase HPLC. Their structures were determined by sequential exoglycosidase digestion and 500 MHz 1H-NMR spectroscopy. Four oligosaccharide structures, A, B, C, and D, were identified as the xylose-containing complex-type. They were present at a molar ratio of 8:1:6:1. By amino acid sequence analyses of the tryptic peptides, Asn-170 and Asn-333 of Cry j I were found to carry asparagine-linked oligosaccharides.
glycoprotein, carbohydrate structure, 1H-NMR, allergen, Cry j I
Structure type: oligomer
Location inside paper: B
Compound class: N-glycan
Contained glycoepitopes: IEDB_114701,IEDB_123886,IEDB_123887,IEDB_130646,IEDB_130701,IEDB_135813,IEDB_136044,IEDB_136045,IEDB_137340,IEDB_137472,IEDB_137485,IEDB_140108,IEDB_140122,IEDB_141793,IEDB_141794,IEDB_141807,IEDB_142489,IEDB_144562,IEDB_144983,IEDB_145668,IEDB_148491,IEDB_148492,IEDB_151531,IEDB_152206,IEDB_152214,IEDB_153212,IEDB_167188,IEDB_167189,IEDB_174332,IEDB_174333,IEDB_190606,IEDB_423128,IEDB_548907,IEDB_983930,SB_165,SB_166,SB_187,SB_195,SB_197,SB_198,SB_30,SB_33,SB_44,SB_67,SB_7,SB_72,SB_73,SB_74,SB_85,SB_86,SB_88
Methods: 1H NMR, HPLC, enzymatic digestion, CC, amino acid sequencing
Comments, role: Parent molecule: Cry j I; proposed structure
NCBI Taxonomy refs (TaxIDs): 3369Reference(s) to other database(s): GTC:G04961MA, CCSD:
47726, CBank-STR:19576
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There is only one chemically distinct structure:
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Hino K, Yamamoto S, Sano O, Taniguchi Y, Kohno K, Usui M, Fukuda S, Hanzawa H, Haruyama H, Kurimoto M
Carbohydrate structures of the glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen
Journal of Biochemistry 117 (1995)
289-295
|
b-D-GlcpNAc-(1-2)-a-D-Manp-(1-3)-+
|
a-L-Fucp-(1-6)-+ | a-L-Fucp-(1-3)-+
| | |
b-D-Galp-(1-4)-b-D-GlcpNAc-(1-2)-a-D-Manp-(1-6)-b-D-Manp-(1-4)-b-D-GlcpNAc-(1-4)-D-GlcpNAc
|
b-D-Xylp-(1-2)-+ |
Show graphically |
Cryptomeria japonica
(NCBI TaxID 3369,
species name lookup)
Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: pollen
NCBI PubMed ID: 7608114Publication DOI: 10.1093/jb/117.2.289Journal NLM ID: 0376600Publisher: Japanese Biochemical Society
Institutions: Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., Okayama
The glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen was treated with pepsin and glycopeptidase A to release asparagine-linked oligosaccharides. The reducing ends of the oligosaccharides were aminated with the fluorescent reagent 2-aminopyridine. The oligosaccharide derivatives were purified by gel permeation chromatography and reversed-phase HPLC. Their structures were determined by sequential exoglycosidase digestion and 500 MHz 1H-NMR spectroscopy. Four oligosaccharide structures, A, B, C, and D, were identified as the xylose-containing complex-type. They were present at a molar ratio of 8:1:6:1. By amino acid sequence analyses of the tryptic peptides, Asn-170 and Asn-333 of Cry j I were found to carry asparagine-linked oligosaccharides.
glycoprotein, carbohydrate structure, 1H-NMR, allergen, Cry j I
Structure type: oligomer
Location inside paper: C
Compound class: N-glycan
Contained glycoepitopes: IEDB_114701,IEDB_115005,IEDB_116644,IEDB_122244,IEDB_123886,IEDB_123887,IEDB_123888,IEDB_130646,IEDB_130701,IEDB_135813,IEDB_136044,IEDB_136045,IEDB_137340,IEDB_137472,IEDB_137485,IEDB_140108,IEDB_140122,IEDB_141793,IEDB_141794,IEDB_141807,IEDB_142489,IEDB_144562,IEDB_144983,IEDB_145668,IEDB_145669,IEDB_146665,IEDB_148491,IEDB_148492,IEDB_148493,IEDB_150092,IEDB_151531,IEDB_152206,IEDB_152214,IEDB_153212,IEDB_167186,IEDB_167188,IEDB_167189,IEDB_174332,IEDB_174333,IEDB_190606,IEDB_423128,IEDB_548907,IEDB_983930,SB_165,SB_166,SB_187,SB_195,SB_197,SB_198,SB_30,SB_33,SB_44,SB_67,SB_7,SB_72,SB_73,SB_74,SB_85,SB_86,SB_88
Methods: 1H NMR, HPLC, enzymatic digestion, CC, amino acid sequencing
Comments, role: Parent molecule: Cry j I; proposed structure
NCBI Taxonomy refs (TaxIDs): 3369Reference(s) to other database(s): GTC:G68766UZ, CCSD:
47727, CBank-STR:19763
Show glycosyltransferases
There is only one chemically distinct structure:
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Hino K, Yamamoto S, Sano O, Taniguchi Y, Kohno K, Usui M, Fukuda S, Hanzawa H, Haruyama H, Kurimoto M
Carbohydrate structures of the glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen
Journal of Biochemistry 117 (1995)
289-295
|
a-L-Fucp-(1-6)-+
|
b-D-Galp-(1-4)-b-D-GlcpNAc-(1-2)-a-D-Manp-(1-6)-+
|
a-L-Fucp-(1-6)-+ | a-L-Fucp-(1-3)-+
| | |
b-D-Galp-(1-4)-b-D-GlcpNAc-(1-2)-a-D-Manp-(1-3)-b-D-Manp-(1-4)-b-D-GlcpNAc-(1-4)-D-GlcpNAc
|
b-D-Xylp-(1-2)-+ |
Show graphically |
Cryptomeria japonica
(NCBI TaxID 3369,
species name lookup)
Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: pollen
NCBI PubMed ID: 7608114Publication DOI: 10.1093/jb/117.2.289Journal NLM ID: 0376600Publisher: Japanese Biochemical Society
Institutions: Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., Okayama
The glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen was treated with pepsin and glycopeptidase A to release asparagine-linked oligosaccharides. The reducing ends of the oligosaccharides were aminated with the fluorescent reagent 2-aminopyridine. The oligosaccharide derivatives were purified by gel permeation chromatography and reversed-phase HPLC. Their structures were determined by sequential exoglycosidase digestion and 500 MHz 1H-NMR spectroscopy. Four oligosaccharide structures, A, B, C, and D, were identified as the xylose-containing complex-type. They were present at a molar ratio of 8:1:6:1. By amino acid sequence analyses of the tryptic peptides, Asn-170 and Asn-333 of Cry j I were found to carry asparagine-linked oligosaccharides.
glycoprotein, carbohydrate structure, 1H-NMR, allergen, Cry j I
Structure type: oligomer
Location inside paper: D
Compound class: N-glycan
Contained glycoepitopes: IEDB_114701,IEDB_115005,IEDB_116644,IEDB_122244,IEDB_123886,IEDB_123887,IEDB_123888,IEDB_130646,IEDB_130701,IEDB_135813,IEDB_136044,IEDB_136045,IEDB_137340,IEDB_137472,IEDB_137485,IEDB_140108,IEDB_140122,IEDB_141793,IEDB_141794,IEDB_141807,IEDB_142489,IEDB_144562,IEDB_144983,IEDB_145668,IEDB_145669,IEDB_146665,IEDB_148491,IEDB_148492,IEDB_148493,IEDB_150092,IEDB_151531,IEDB_152206,IEDB_152214,IEDB_153212,IEDB_167186,IEDB_167188,IEDB_167189,IEDB_174332,IEDB_174333,IEDB_190606,IEDB_423128,IEDB_540672,IEDB_548907,IEDB_983930,SB_165,SB_166,SB_187,SB_195,SB_197,SB_198,SB_30,SB_33,SB_44,SB_67,SB_7,SB_72,SB_73,SB_74,SB_85,SB_86,SB_88
Methods: 1H NMR, HPLC, enzymatic digestion, CC, amino acid sequencing
Comments, role: Parent molecule: Cry j I; proposed structure
NCBI Taxonomy refs (TaxIDs): 3369Reference(s) to other database(s): GTC:G62500OS, CCSD:
47728, CBank-STR:20831
Show glycosyltransferases
There is only one chemically distinct structure:
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