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Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)

NCBI PubMed ID: 2663842
Journal NLM ID: 2985121R
Publisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Institutions: Department of Biochemistry, University of California, Berkeley 94720

We find that the N-linked Man8GlcNAc2- core oligosaccharide of Saccharomyces cerevisiae mnn mutant mannoproteins is enlarged by the addition of the outer chain to the α 1----3-linked mannose in the side chain that is attached to the β 1----4-linked mannose rather than by addition to the terminal α 1----6-linked mannose. This conclusion is derived from structural studies on a phosphorylated oligosaccharide fraction and from mass spectral fragment analysis of neutral core oligosaccharides.

Structure type: oligomer
Location inside paper: abstract, structure I
Compound class: N-glycan, high-mannose
Contained glycoepitopes: IEDB_130701,IEDB_135813,IEDB_136104,IEDB_137340,IEDB_137485,IEDB_140116,IEDB_141793,IEDB_141807,IEDB_141828,IEDB_141829,IEDB_141830,IEDB_141831,IEDB_143632,IEDB_144983,IEDB_151079,IEDB_151531,IEDB_152206,IEDB_153220,IEDB_153762,IEDB_164174,IEDB_429156,IEDB_857734,IEDB_857735,IEDB_983930,SB_136,SB_191,SB_196,SB_197,SB_198,SB_44,SB_53,SB_67,SB_72,SB_73,SB_77

Methods: 1H NMR, gel filtration, HPLC, acetolysis, methylation analysis, reductive amination, Fab-MS, endo-N-acetylglucosaminidase H digestion, gradient elution, LSIMS
Comments, role: core oligosaccharide of the mnn1 mnn2 mutant mannoprotein

Related record ID(s): 134686, 135561, 135677, 135678, 226529
NCBI Taxonomy refs (TaxIDs): 4932
Reference(s) to other database(s): GTC:G70533NF, CCSD:29433, CBank-STR:21247
Show glycosyltransferases

There are 2 chemically distinct structures. Please, select:

CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO[C@H]3O[C@H](CO[C@H]4O[C@H](CO[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]5O)[C@@H](O)[C@H](O)[C@@H]4O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H](O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H]3O)[C@@H](O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]3O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]3O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]3O)[C@@H]2O)[C@@H]1O

SVG MW SMILES 3D mol Ionize

Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)

The structure was elucidated in this paper
NCBI PubMed ID: 2663842
Journal NLM ID: 2985121R
Publisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Institutions: Department of Biochemistry, University of California, Berkeley 94720

Structure type: oligomer
Location inside paper: abstract, structure II
Compound class: N-glycan
Contained glycoepitopes: IEDB_130701,IEDB_135813,IEDB_136104,IEDB_137340,IEDB_137485,IEDB_140116,IEDB_141793,IEDB_141807,IEDB_141828,IEDB_141829,IEDB_141830,IEDB_141831,IEDB_143632,IEDB_144983,IEDB_151079,IEDB_151531,IEDB_152206,IEDB_153220,IEDB_164174,IEDB_429156,IEDB_857734,IEDB_983930,SB_136,SB_191,SB_196,SB_197,SB_198,SB_44,SB_53,SB_67,SB_72,SB_73,SB_77

Methods: 1H NMR, gel filtration, HPLC, acetolysis, methylation analysis, reductive amination, Fab-MS, endo-N-acetylglucosaminidase H digestion, gradient elution, LSIMS
Comments, role: core oligosaccharide of the mnn1 mnn2 and mnn1 mnn2 mnn10 mutant mannoproteins

Related record ID(s): 115954, 126512, 135676, 135678, 135713, 136216
NCBI Taxonomy refs (TaxIDs): 4932
Reference(s) to other database(s): GTC:G86072AZ, CCSD:29436, CBank-STR:19075
Show glycosyltransferases

There are 2 chemically distinct structures. Please, select:

CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO[C@H]3O[C@H](CO[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H](O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H]3O)[C@@H](O)[C@H](O[C@H]3O[C@H](CO[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H](O)[C@H](O)[C@@H]3O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]3O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]3O)[C@@H]2O)[C@@H]1O

SVG MW SMILES 3D mol Ionize

Structure type: oligomer
Location inside paper: abstract, structure III
Compound class: N-glycan
Contained glycoepitopes: IEDB_130701,IEDB_135813,IEDB_136104,IEDB_137340,IEDB_137485,IEDB_140116,IEDB_141793,IEDB_141807,IEDB_141828,IEDB_141829,IEDB_141830,IEDB_141831,IEDB_143632,IEDB_144983,IEDB_151079,IEDB_151531,IEDB_152206,IEDB_153220,IEDB_164174,IEDB_429156,IEDB_857734,IEDB_983930,SB_136,SB_191,SB_196,SB_197,SB_198,SB_44,SB_53,SB_67,SB_72,SB_73,SB_77

Methods: 1H NMR, gel filtration, HPLC, acetolysis, methylation analysis, reductive amination, Fab-MS, endo-N-acetylglucosaminidase H digestion, gradient elution, LSIMS
Comments, role: core oligosaccharide of the mnn1 mnn9 mutant mannoprotein

Related record ID(s): 107561, 115956, 126518, 135676, 135677, 136221, 147596
NCBI Taxonomy refs (TaxIDs): 4932
Reference(s) to other database(s): GTC:G21666ZL, CCSD:29440, CBank-STR:19753
Show glycosyltransferases

There are 2 chemically distinct structures. Please, select:

SVG MW SMILES 3D mol Ionize