A special levan-type exopolysaccharide (EPS) from Bacillus amyloliquefaciens JN4 with antiadhesive activity against enterotoxigenic Escherichia coli (ETEC) was purified and identified. Chemical analysis indicated that EPS-JN4 with a low molecular weight of 8 kDa is composed of fructose and glucose with a molar ratio of 46.1:1. Structural analysis clarified that EPS-JN4 contains a main chain of β-(2,6)-linked Fruf residues and intensive branches of a single 2-linked Fruf at every six residues. Furthermore, the superior antiadhesive activity of EPS-JN4 against ETEC showed its potential usage as an antiadhesive agent for diarrhea prevention. EPS-JN4 is a specific type of levan family, for its small molecular size and intensive branches. The results expand the knowledge on structural types of levan and illustrate its potential as an antiadhesive agent for diarrhea prevention, which will be conducive to elucidate the relation between structure and function.
enterotoxigenic, exopolysaccharide, enterotoxigenic Escherichia coli, levan, antiadhesive, Bacillus amyloliquefaciens
NCBI PubMed ID: 31246026Publication DOI: 10.1021/acs.jafc.9b03234Journal NLM ID: 0374755Publisher: American Chemical Society
Correspondence: xiaomin@jiangnan.edu.cn; jlu@jiangnan.edu.cn
Institutions: The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi 214122, P. R. China, National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122 , P. R. China, Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, P. R. China, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122 , P. R. China
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, IR, GC-MS, sugar analysis, HPAEC-PAD, hemagglutination, HPGFC
Found