Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: pectin
Journal NLM ID: 0427043Publisher: Amsterdam: Elsevier
Institutions: Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Czechoslovakia, Department of Biochemistry, Charles University, Prague, Czechoslovakia
Oligo-D-galactosiduronic acids of different degrees of polymerization (DP 2, 4 and 5) were O-glycosidically bound to a poly(hydroxyethyl methacrylate) gel, Separon. Their ability to function as biospecific ligands of endopolygalacturonase was studied by affinity chromatography. Endopolygalacturonases ofAspergillus niger and from tomato were selectively adsorbed by Separon derivatives with tetra- and pentagalactosiduronic acids, whereas digalactosiduronic acid—Separon retained only a fraction of the enzyme. Adsorbed endopolygalacturonase was quantitatively displaced from the adsorbents either by changing the pH or by bioelution with oligogalactosiduronides of DP > 2. The dissociation constants of the affinity complexes (KL) as determined by zonal analysis were close to the values for the soluble complexes (KI) with counter-ligands of the same DP.
Structure type: homopolymer ; n=1-3
Compound class: galacturonan
Methods: affinity chromatography
Comments, role: source unverified
NCBI Taxonomy refs (TaxIDs): 2706Reference(s) to other database(s): GTC:G86267DW, CCSD:
16799, CBank-STR:5594
Show glycosyltransferases
There is only one chemically distinct structure:
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