Mbawala A, Mahmood SA, Loppinet V, Bonaly R Acetolysis and proton NMR studies on mannans isolated from very flocculent and weakly flocculent cells of Pichia pastoris IFP 206 Journal of General Microbiology136 (1990)
1279-1284
The structure was elucidated in this paper NCBI PubMed ID:2230716 Journal NLM ID:0375371 Institutions: Laboratoire de Biochimie Microbienne, Faculté de Pharmacie, Université de Nancy, Nancy, France
Growth of the yeast Pichia pastoris IFP 206 in methanol- and glucose-containing media led respectively to very and weakly flocculent cells. Mannans from both kinds of cells were extracted and compared. Chemical analysis and molecular mass estimation showed some differences between the mannans from very and weakly flocculent cells, especially in quantitative amino acid content. 1H NMR analysis showed that both kinds of mannan contained α-1,2 and β-1,2 linkages. Two acetolysis conditions, combined with 1H NMR analysis, revealed that mannans from both kinds of cells were composed of mannose, mannobiose, mannotriose, mannotetraose and mannopentaose side-chains with the following respective structures: Man; Man α 1---2Man; Man α 1----2Man α 1----2Man; Man β 1----2Man α 1----2Man; Man β 1----2Man β 1----2Man α 1----2Man; Man α 1----2Man β 1----2Man β 1----2Man α 1----2Man. Additionally the β-1,2 linkages of the non-reducing terminal residues of the mannotetraose were shown to be acetolysis-labile. The mannans from very flocculent cells were richer in mannopentaose than the mannans from weakly flocculent cells. According to these results, the extended conformations in the branching moieties of the mannan could be the basis of the higher degree of flocculation of the methanol-grown cells.
Mbawala A, Mahmood SA, Loppinet V, Bonaly R Acetolysis and proton NMR studies on mannans isolated from very flocculent and weakly flocculent cells of Pichia pastoris IFP 206 Journal of General Microbiology136 (1990)
1279-1284
The structure was elucidated in this paper NCBI PubMed ID:2230716 Journal NLM ID:0375371 Institutions: Laboratoire de Biochimie Microbienne, Faculté de Pharmacie, Université de Nancy, Nancy, France
Growth of the yeast Pichia pastoris IFP 206 in methanol- and glucose-containing media led respectively to very and weakly flocculent cells. Mannans from both kinds of cells were extracted and compared. Chemical analysis and molecular mass estimation showed some differences between the mannans from very and weakly flocculent cells, especially in quantitative amino acid content. 1H NMR analysis showed that both kinds of mannan contained α-1,2 and β-1,2 linkages. Two acetolysis conditions, combined with 1H NMR analysis, revealed that mannans from both kinds of cells were composed of mannose, mannobiose, mannotriose, mannotetraose and mannopentaose side-chains with the following respective structures: Man; Man α 1---2Man; Man α 1----2Man α 1----2Man; Man β 1----2Man α 1----2Man; Man β 1----2Man β 1----2Man α 1----2Man; Man α 1----2Man β 1----2Man β 1----2Man α 1----2Man. Additionally the β-1,2 linkages of the non-reducing terminal residues of the mannotetraose were shown to be acetolysis-labile. The mannans from very flocculent cells were richer in mannopentaose than the mannans from weakly flocculent cells. According to these results, the extended conformations in the branching moieties of the mannan could be the basis of the higher degree of flocculation of the methanol-grown cells.
Methods: 1H NMR, gel filtration, GLC, paper chromatography, acetolysis, ethanol precipitation, HCl hydrolysis, thin-layer chromatography, autoclaving in sodium citrate buffer, phenol/sulphuric acid method of Dubois, micromethod of Ghuysen, method of McClare Comments, role: degradation product
Related record ID(s): 104303, 104351, 139836, 139838, 139839 NCBI Taxonomy refs (TaxIDs):4922 Reference(s) to other database(s): GTC:G12486QZ, CCSD:29812, CBank-STR:5217 Show glycosyltransferases
There are 3 chemically distinct structures. Please, select:
Mbawala A, Mahmood SA, Loppinet V, Bonaly R Acetolysis and proton NMR studies on mannans isolated from very flocculent and weakly flocculent cells of Pichia pastoris IFP 206 Journal of General Microbiology136 (1990)
1279-1284
The structure was elucidated in this paper NCBI PubMed ID:2230716 Journal NLM ID:0375371 Institutions: Laboratoire de Biochimie Microbienne, Faculté de Pharmacie, Université de Nancy, Nancy, France
Growth of the yeast Pichia pastoris IFP 206 in methanol- and glucose-containing media led respectively to very and weakly flocculent cells. Mannans from both kinds of cells were extracted and compared. Chemical analysis and molecular mass estimation showed some differences between the mannans from very and weakly flocculent cells, especially in quantitative amino acid content. 1H NMR analysis showed that both kinds of mannan contained α-1,2 and β-1,2 linkages. Two acetolysis conditions, combined with 1H NMR analysis, revealed that mannans from both kinds of cells were composed of mannose, mannobiose, mannotriose, mannotetraose and mannopentaose side-chains with the following respective structures: Man; Man α 1---2Man; Man α 1----2Man α 1----2Man; Man β 1----2Man α 1----2Man; Man β 1----2Man β 1----2Man α 1----2Man; Man α 1----2Man β 1----2Man β 1----2Man α 1----2Man. Additionally the β-1,2 linkages of the non-reducing terminal residues of the mannotetraose were shown to be acetolysis-labile. The mannans from very flocculent cells were richer in mannopentaose than the mannans from weakly flocculent cells. According to these results, the extended conformations in the branching moieties of the mannan could be the basis of the higher degree of flocculation of the methanol-grown cells.
Methods: 1H NMR, gel filtration, GLC, paper chromatography, acetolysis, ethanol precipitation, HCl hydrolysis, thin-layer chromatography, autoclaving in sodium citrate buffer, phenol/sulphuric acid method of Dubois, micromethod of Ghuysen, method of McClare Comments, role: degradation product
Related record ID(s): 104304, 104353, 139836, 139837, 139839 NCBI Taxonomy refs (TaxIDs):4922 Reference(s) to other database(s): GTC:G23356VB, CCSD:29814, CBank-STR:10339 Show glycosyltransferases
There are 3 chemically distinct structures. Please, select:
Mbawala A, Mahmood SA, Loppinet V, Bonaly R Acetolysis and proton NMR studies on mannans isolated from very flocculent and weakly flocculent cells of Pichia pastoris IFP 206 Journal of General Microbiology136 (1990)
1279-1284
The structure was elucidated in this paper NCBI PubMed ID:2230716 Journal NLM ID:0375371 Institutions: Laboratoire de Biochimie Microbienne, Faculté de Pharmacie, Université de Nancy, Nancy, France
Growth of the yeast Pichia pastoris IFP 206 in methanol- and glucose-containing media led respectively to very and weakly flocculent cells. Mannans from both kinds of cells were extracted and compared. Chemical analysis and molecular mass estimation showed some differences between the mannans from very and weakly flocculent cells, especially in quantitative amino acid content. 1H NMR analysis showed that both kinds of mannan contained α-1,2 and β-1,2 linkages. Two acetolysis conditions, combined with 1H NMR analysis, revealed that mannans from both kinds of cells were composed of mannose, mannobiose, mannotriose, mannotetraose and mannopentaose side-chains with the following respective structures: Man; Man α 1---2Man; Man α 1----2Man α 1----2Man; Man β 1----2Man α 1----2Man; Man β 1----2Man β 1----2Man α 1----2Man; Man α 1----2Man β 1----2Man β 1----2Man α 1----2Man. Additionally the β-1,2 linkages of the non-reducing terminal residues of the mannotetraose were shown to be acetolysis-labile. The mannans from very flocculent cells were richer in mannopentaose than the mannans from weakly flocculent cells. According to these results, the extended conformations in the branching moieties of the mannan could be the basis of the higher degree of flocculation of the methanol-grown cells.
Methods: 1H NMR, gel filtration, GLC, paper chromatography, acetolysis, ethanol precipitation, HCl hydrolysis, thin-layer chromatography, autoclaving in sodium citrate buffer, phenol/sulphuric acid method of Dubois, micromethod of Ghuysen, method of McClare Comments, role: degradation product
Related record ID(s): 104305, 139836, 139837, 139838 NCBI Taxonomy refs (TaxIDs):4922 Reference(s) to other database(s): GTC:G60768JL, CCSD:22042, CBank-STR:4648 Show glycosyltransferases
There are 3 chemically distinct structures. Please, select:
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(later renamed to: Komagataella pastoris IFP 206)