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Taxonomic group: plant / Streptophyta (Phylum: Streptophyta)
Organ / tissue: suspension culture

Publication DOI: 10.1016/S0176-1617(11)81980-2
Journal NLM ID: 9882059
Publisher: Urban & Fischer
Institutions: Botanisches Institut der Universität Heidelberg, Heidelberg, Germany

Media isolated from growing cell cultures (conditioned media) contain «viability factors» which promote the survival of plant cells plated at low density. We developed a procedure for the purification of the viability factor (VF) of tobacco. Separated by HPLC, a peak of VF-activity correlated with a peak of refractive index. Assuming that the RI-peak is only caused by the VF, its concentration in the conditioned medium is between 10-8-10-9 M. We found that the viability factor is a branched oligosaccharide with a molecular weight of about 1,000 Da. It consists of a «backbone» of three α-1,4-glycosidic-linked galacturonic acids, at least two non-reducing ends (a β-linked glucose and an α-1,2/1,3 or 1,4-linked fucose) and an unidentified monomer, which is positively charged at pH 4. The pectic backbone is responsible for the negative charge at pH 9.5 and probably for its acid lability. The purification procedure which purifies the VF of tobacco almost to homogeneity was also applicable to the VF of carrot. Therefore both factors, from species of different families, have a similar structure. The only difference found was that the VF of carrot is about 200 Da bigger than the VF of tobacco. No structural similarities with other oligosaccharides could be found.

oligosaccharide, cell suspension culture, Nicotiana, Daucus, conditioning factors, low cell density, viability factor

Structure type: oligomer
Location inside paper: Fig. 5
Trivial name: tobacco viability factor
Contained glycoepitopes: IEDB_115015,IEDB_136045,IEDB_142488,IEDB_142489,IEDB_144562,IEDB_146664,IEDB_149135,IEDB_152214,IEDB_174333,IEDB_983931,SB_192,SB_86

Methods: enzymatic degradation, HPLC, bioassay, alkaline electrodialysis
Comments, role: proposed structure of capping moiety; Fuc attachment is 2,3 or 4.

NCBI Taxonomy refs (TaxIDs): 4097
Reference(s) to other database(s): CCSD:49100, CBank-STR:10221
Show glycosyltransferases

There are too many chemically distinct structures (~384), so only one is shown:

bDGlcp(1-2)[aDFucp(1-3)]aDGalpA(1-4)aDGalpA(1-4)aDGalpA(1-?)ANY

[1*]O[C@H]1O[C@H](C(=O)O)[C@H](O[C@H]2O[C@H](C(=O)O)[C@H](O[C@H]3O[C@H](C(=O)O)[C@H](O)[C@H](O[C@H]4O[C@H](C)[C@H](O)[C@H](O)[C@H]4O)[C@H]3O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)[C@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize

R1 = ANY