A new tetrasaccharide derivative, actinotetraose L (1), was isolated from the methanol extract of the mycelia of the grasshopper-associated rare actinobacterium Amycolatopsis sp. HCa1. The structure of the new compound was elucidated by a combination of 1D, 2D NMR (HMQC, HMBC, COSY, and NOESY), and HR-ESI-MS analyses as O-{3,4-di-O-[(E)-2-ethyl-2-butenoyl]-β-D-glucopyranosyl-(1→2)}-O-{3,4-di-O-[(E)-2-methyl-2-butenoyl]-β-D-glucopyranosyl-(1→2)}-6,6-di-O-[(E)-2-methyl-2-butenoyl]-α-D-glucopyranosyl-(1→1)-α-D-glucopyranoside. The immunosuppressive activity and cytotoxicity of the compound were evaluated by T-cell viability and MTT assays. Nonetheless, no significant activity was observed
tetrasaccharide, Amycolatopsis, Pseudonocardiaceae, grasshopper
NCBI PubMed ID: 26218137Publication DOI: 10.1080/10286020.2015.1052413Journal NLM ID: 100888334Publisher: Harwood Academic Publishers; London: Informa Healthcare
Correspondence: Ge HM
Institutions: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China, Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China
Methods: 13C NMR, 1H NMR, NMR-2D, IR, TLC, HPLC, UV, extraction, optical rotation measurement, CC, cell growth, HR-ESI-MS, HPLC-DAD
Found