Publication DOI:10.1016/S0031-9422(00)94629-6 Journal NLM ID:0151434 Publisher: Elsevier Institutions: Department of Botany and Plant Pathology, Iowa State University, Ames, IA, USA
Structural features of noncellulosic β-d-glucans of Zea mays, Hordeum vulgare, Triticum vulgare, Secale cereale, and Sorghum bicolor were compared. Treatment of cell walls derived from these species with specific Bacillus subtilis or Rhizopus glucanases yields virtually identical profiles upon Bio-Gel P-2 fractionation of the liberated oligosaccharides. The two predominant reaction products, a trisaccharide and tetrasaccharide, were identified as 3-O-β-cellobiosyl-d-glucose and 3-O-β-cellotriosyl-d-glucose respectively by virtue of the specificity of these enzymes and by paper chromatography and electrophoresis. The similarity of the reaction product profiles indicates a rather regular repeating sequence in all β-d-glucans examined. The ratios of 3-O-β-cellobiosyl-d-glucose to 3-O-β-cellotriosyl-d-glucose indicates that 30.4–30.9% of the β-glucosyl linkages in the intact molecule are 1→3. The yields of wall glucan as estimated from the quantity of oligosaccharides released, range from 41 μg/mg wall in Hordeum to 97 μg/mg wall from Sorghum.
Publication DOI:10.1016/S0031-9422(00)94629-6 Journal NLM ID:0151434 Publisher: Elsevier Institutions: Department of Botany and Plant Pathology, Iowa State University, Ames, IA, USA
Structural features of noncellulosic β-d-glucans of Zea mays, Hordeum vulgare, Triticum vulgare, Secale cereale, and Sorghum bicolor were compared. Treatment of cell walls derived from these species with specific Bacillus subtilis or Rhizopus glucanases yields virtually identical profiles upon Bio-Gel P-2 fractionation of the liberated oligosaccharides. The two predominant reaction products, a trisaccharide and tetrasaccharide, were identified as 3-O-β-cellobiosyl-d-glucose and 3-O-β-cellotriosyl-d-glucose respectively by virtue of the specificity of these enzymes and by paper chromatography and electrophoresis. The similarity of the reaction product profiles indicates a rather regular repeating sequence in all β-d-glucans examined. The ratios of 3-O-β-cellobiosyl-d-glucose to 3-O-β-cellotriosyl-d-glucose indicates that 30.4–30.9% of the β-glucosyl linkages in the intact molecule are 1→3. The yields of wall glucan as estimated from the quantity of oligosaccharides released, range from 41 μg/mg wall in Hordeum to 97 μg/mg wall from Sorghum.
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