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Structure type: polymer biological repeating unit
Compound class: CPS
Contained glycoepitopes: IEDB_130648,IEDB_137340,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_141807,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_885822,IEDB_983931,SB_192

• Article ID: 5791
  Knirel YA, Van Calsteren M "Bacterial exopolysaccharides" - Book: Comprehensive Glycoscience: From Chemistry to Systems Biology. Reference Module in Chemistry, Molecular Sciences and Chemical Engineering (2021) 1-75
  

  Bacterial extracellular polysaccharides are known as a cell-bound capsule, a sheath, or a slime, which is excreted into the environment. They play an important role in virulence of medical bacteria and plant-to-symbiont interaction and are used for serotyping of bacteria and production of vaccines. Some exopolysaccharides have commercial applications in industry, and claims of health benefits have been documented for an increasing number of them. Exopolysaccharides have diverse composition and structure, and some contain sugar and non-sugar components that are found in bacterial carbohydrates only. The present article provides an updated collection of the data on exopolysaccharides of various classes of gram-negative and gram-positive bacteria reported until the end of 2019. When known, biosynthesis pathways of exopolysaccharides are treated in a summary manner. References are made to structure and biosynthesis relatedness between exopolysaccharides of different bacterial taxa as well as between bacterial polysaccharides and mammalian glycosaminoglycans.

  polysaccharide structure, Gram-negative bacteria, capsule, Biofilm, polysaccharide biosynthesis, gram-positive bacteria, Monosaccharide composition, Bacterial exopolysaccharide, non-sugar component

  Publication DOI: 10.1016/B978-0-12-819475-1.00005-5
  Publisher: Elsevier
  Correspondence: marie-rose.vancalsteren@canada.ca; yknirel@gmail.com
  Editors: Barchi J, Kamerling H
  Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Saint-Hyacinthe Research and Development Centre, Agriculture and Agri-Food Canada, Saint-Hyacinthe, QC, Canada
  
   
  
  Acinetobacter baumannii K47 NIPH 601
  CSDB ID 6741 (all data & tools)
• Article ID: 6140
  Shchurova AS, Shneider MM, Arbatsky NP, Shashkov AS, Chizhov AO, Skryabin YP, Mikhailova YV, Sokolova OS, Shelenkov AA, Miroshnikov KA, Knirel YA, Popova AV "Novel Acinetobacter baumannii Myovirus TaPaz Encoding Two Tailspike Depolymerases: Characterization and Host-Recognition Strategy" - Viruses 13(6) (2021) 978
  

  Acinetobacter baumannii, one of the most significant nosocomial pathogens, is capable of producing structurally diverse capsular polysaccharides (CPSs) which are the primary receptors for A. baumannii bacteriophages encoding polysaccharide-degrading enzymes. To date, bacterial viruses specifically infecting A. baumannii strains belonging to more than ten various capsular types (K types) were isolated and characterized. In the present study, we investigate the biological properties, genomic organization, and virus-bacterial host interaction strategy of novel myovirus TaPaz isolated on the bacterial lawn of A. baumannii strain with a K47 capsular polysaccharide structure. The phage linear double-stranded DNA genome of 93,703 bp contains 178 open reading frames. Genes encoding two different tailspike depolymerases (TSDs) were identified in the phage genome. Recombinant TSDs were purified and tested against the collection of A. baumannii strains belonging to 56 different K types. One of the TSDs was demonstrated to be a specific glycosidase that cleaves the K47 CPS by the hydrolytic mechanism.

  Acinetobacter baumannii, capsular polysaccharide, bacteriophage, glycosidase, capsular type, tailspike depolymerase

  NCBI PubMed ID: 34070371
  Publication DOI: 10.3390/v13060978
  Journal NLM ID: 101509722
  Publisher: Basel, Switzerland: MDPI
  Correspondence: popova_nastya86@mail.ru
  Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Moscow Institute of Physics and Technology (National Research University), Institutskiy per. 9, Dolgoprudny, 141700 Moscow Region, Russia, State Research Center for Applied Microbiology and Biotechnology, 24 'Quarter A' Territory, Obolensk, City District Serpukhov, 142279 Moscow Region, Russia, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia, Central Scientific Research Institute of Epidemiology, Novogireevskaya 3a, 111123 Moscow, Russia, Biology Department, Lomonosov Moscow State University, Leninskie Gory 1, 119234 Moscow, Russia, Biology Department, Shenzhen MSU-BIT University, Ruyi Rd. 299, Longgang District, Shenzhen 518172, China
  Methods: 13C NMR, 1H NMR, NMR-2D, DNA techniques, GPC, phage depolymerisation, HR-ESI-MS, TEM, phage isolation, phage infection inhibition assay, phage genome analysis
  
   
  
  Acinetobacter baumannii K47 NIPH 601
  CSDB ID 10940 (all data & tools)