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Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)

The structure was elucidated in this paper
NCBI PubMed ID: 7937765
Publication DOI: 10.1073/pnas.91.20.9327
Journal NLM ID: 7505876
Publisher: National Academy of Sciences
Institutions: Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA, Department of Molecular and Cell Biology, University of California, Berkeley 94720

Mutagenesis of Schizosaccharomyces pombe cells yielded a strain that made reduced amounts of invertase. A comparison of the O- and N-linked carbohydrate chains of the wild-type and mutant glycoproteins revealed that a single type of α 1→2-linked mannose was missing in the mutant. Analysis of the wild-type galactomannoprotein showed that it contained a heterogeneous small "core" oligosaccharide fraction linked to asparagine with sugar compositions that ranged from Man9(GlcNAc)2- to Gal4Man10(GlcNAc)2-. The galactose units are in terminal positions of a Man10(GlcNAc)2- unit that is similar to the mannoprotein core of Saccharomyces cerevisiae. Attached to this core in a larger oligosaccharide fraction is an α 1→6-linked polymannose chain that is substituted at position 2 with α-linked mannose and galactose. The O-linked sugars consist of mannose, α 1→2-linked mannosylmannose and α 1→2-linked galactosylmannose, along with small amounts of tri- and tetrasaccharides. The glycosylation mutant lacks α 1→2-linked mannose on both the O-linked chains and the outer chain of the large N-linked chains, suggesting that it may be defective in regulation of an α-1,2-mannosyltransferase that adds mannose to glycoproteins in the Golgi.

Structure type: oligomer
Location inside paper: Table 1, A
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130701,IEDB_135813,IEDB_136104,IEDB_137340,IEDB_137485,IEDB_140116,IEDB_141793,IEDB_141807,IEDB_141828,IEDB_141829,IEDB_141830,IEDB_141831,IEDB_143632,IEDB_144983,IEDB_151079,IEDB_151531,IEDB_152206,IEDB_153220,IEDB_164174,IEDB_429156,IEDB_857734,IEDB_983930,SB_136,SB_191,SB_196,SB_197,SB_198,SB_44,SB_53,SB_67,SB_72,SB_73,SB_77

Methods: 1H NMR, periodate oxidation, gel filtration, HPLC, anion exchange chromatography, Cetavlon treatment, acetic hydrolysis
Biosynthesis and genetic data: genetic data
Comments, role: Parent molecule: N-glycan, mannoprotein; core structure for ID147678

Related record ID(s): 147677, 147678, 197678
NCBI Taxonomy refs (TaxIDs): 4896
Reference(s) to other database(s): GTC:G65311MC, CCSD:35689, CBank-STR:19728
Show glycosyltransferases

There is only one chemically distinct structure:

CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO[C@H]3O[C@H](CO[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H](O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H]3O)[C@@H](O)[C@H](O[C@H]3O[C@H](CO[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H](O)[C@H](O)[C@@H]3O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]3O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]3O)[C@@H]2O)[C@@H]1O

SVG MW SMILES 3D mol Ionize

Structure type: oligomer
Location inside paper: Table 1, B
Compound class: N-glycan, mannoprotein
Contained glycoepitopes: IEDB_130701,IEDB_131186,IEDB_135813,IEDB_135818,IEDB_136104,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137485,IEDB_140116,IEDB_141793,IEDB_141794,IEDB_141807,IEDB_141828,IEDB_141829,IEDB_141830,IEDB_141831,IEDB_143632,IEDB_144983,IEDB_151079,IEDB_151528,IEDB_151531,IEDB_152206,IEDB_153220,IEDB_164174,IEDB_190606,IEDB_429156,IEDB_857734,IEDB_983930,SB_136,SB_191,SB_196,SB_197,SB_198,SB_44,SB_53,SB_67,SB_7,SB_72,SB_73,SB_77

Methods: 1H NMR, periodate oxidation, gel filtration, HPLC, anion exchange chromatography, Cetavlon treatment, acetic hydrolysis
Biosynthesis and genetic data: genetic data
Comments, role: Parent molecule: N-glycan, mannoprotein; core structure for ID147678.

Related record ID(s): 147676, 147678, 197678
NCBI Taxonomy refs (TaxIDs): 4896
Reference(s) to other database(s): GTC:G78358RF, CCSD:35690, CBank-STR:21593
Show glycosyltransferases

There is only one chemically distinct structure:

CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO[C@H]3O[C@H](CO[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O[C@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4O[C@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4O)[C@@H](O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O[C@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4O[C@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4O)[C@@H]3O)[C@@H](O)[C@H](O[C@H]3O[C@H](CO[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H](O)[C@H](O)[C@@H]3O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]3O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]3O[C@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O[C@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O)[C@@H]2O)[C@@H]1O

SVG MW SMILES 3D mol Ionize

Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)

NCBI PubMed ID: 7937765
Publication DOI: 10.1073/pnas.91.20.9327
Journal NLM ID: 7505876
Publisher: National Academy of Sciences
Institutions: Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA, Department of Molecular and Cell Biology, University of California, Berkeley 94720

Structure type: structural motif or average structure
Location inside paper: Fig. 6
Aglycon: protein
Compound class: N-glycan, mannoprotein
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_136906,IEDB_137472,IEDB_140116,IEDB_141793,IEDB_141794,IEDB_141828,IEDB_141829,IEDB_141831,IEDB_143632,IEDB_144983,IEDB_151528,IEDB_152206,IEDB_153220,IEDB_190606,IEDB_76933,IEDB_983930,SB_136,SB_191,SB_196,SB_198,SB_44,SB_67,SB_7,SB_72

Methods: 1H NMR, periodate oxidation, gel filtration, HPLC, anion exchange chromatography, Cetavlon treatment, acetic hydrolysis
Comments, role: The length of the outer chain of N-linked oligosaccharides is unknown, but the mannose, mannobiose, and galactosylmannose are present in the ratio 1:1:1.65

Related record ID(s): 147676, 147677, 197678
NCBI Taxonomy refs (TaxIDs): 4896
Reference(s) to other database(s): CCSD:35691, CBank-STR:13041
Show glycosyltransferases

There is only one chemically distinct structure:

CC(=O)N[C@H]1C(NC(=O)CC(N)C(=O)O)O[C@H](CO)[C@@H](O[1*]O[C@H]2O[C@H](CO[C@H]3O[C@H](CO[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H](O)[C@H](O)[C@@H]3O[C@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O)[C@@H](O)[C@H](O)[C@@H]2O)[C@@H]1O

SVG MW SMILES 3D mol Ionize

R1 = core, ID 147676 or ID 147677

Structure type: oligomer
Location inside paper: Fig. 6
Aglycon: ->3)Ser/Thr
Compound class: O-glycan
Contained glycoepitopes: IEDB_130701,IEDB_131186,IEDB_135818,IEDB_136104,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_143632,IEDB_144983,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_7,SB_72

Methods: 1H NMR, periodate oxidation, gel filtration, HPLC, anion exchange chromatography, Cetavlon treatment, acetic hydrolysis

Related record ID(s): 147676, 147677, 147678
NCBI Taxonomy refs (TaxIDs): 4896
Show glycosyltransferases

There is only one chemically distinct structure:

OC[C@H]1O[C@H](O)[C@@H](O)[C@@H](O)[C@@H]1O

SVG MW SMILES 3D mol Ionize